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pCfB3049(gRNA XII-4)

Plasmid

#73291

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XII-4

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

guiding RNA

Use

CRISPR; Grna

Expression

Yeast

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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MTK3b_020

Plasmid

#123801

Purpose

Encodes synTF ZF binding domain as a Type 3b part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

SynTF

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pSUPERpuro-BglII-Hs4E-T_AC

Plasmid

#148445

Purpose

Mammalian Expression of Hs4E-T-shRNA

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

Hs4E-T-shRNA (EIF4ENIF1 Human)

Expression

Mammalian

Available Since

Aug. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pWP1_mFTCD

Plasmid

#102317

Purpose

expression of mouse cDNA of FTCD, refractory to the guides in plasmids 1,2

Depositor

David Sabatini

Article

Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

Ftcd (Ftcd Mouse)

Use

Lentiviral

Promoter

EF1alpha

Available Since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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Short fibulin-3-V5 pcDNA4

Plasmid

#29702

Depositor

Mariano Viapiano

Article

Hu et al Mol Cancer Res. 2009 Nov . 7(11):1756-70

Insert

EFEMP1 (EFEMP1 Human)

Tags

V5

Expression

Mammalian

Mutation

lacks N-terminus of EFEMP1 (amino acids 1-105)

Available Since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only
pLentiCRISPR sgHAL

Plasmid

#102315

Purpose

genetic depletion of HAL

Depositor

David Sabatini

Article

Kanarek et al Nature. 2018 Jul 11. pii: 10.1038/s41586

Insert

sgHAL (HAL Human)

Use

Lentiviral

Promoter

U6

Available Since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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MTK3a_001

Plasmid

#123754

Purpose

Encodes plasma membrane localization signal from Lyn kinase as a Type 3a part to be used in the MTK

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

LYN

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK3a_006

Plasmid

#123759

Purpose

Encodes ER Targeting Sequence (N Terminal) as a Type 3a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

ETS

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK4a_014

Plasmid

#123827

Purpose

Encodes a nuclear localized mAzamiGreen coexpression cassette as a Type 4a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

P2A::NLS::mAzamiGreen

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK0_018

Plasmid

#123933

Purpose

Encodes the spacer TU with connectors LS R1 as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

TULSR1 - spacer

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK0_019

Plasmid

#123934

Purpose

Encodes the spacer TU with connectors L1 R2 as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

TUL1R2 spacer

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK0_020

Plasmid

#123935

Purpose

Encodes the spacer TU with connectors L2 R3 as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

TUL2R3 spacer

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK0_022

Plasmid

#123937

Purpose

Encodes the spacer TU with connectors L4 R5 as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

TUL4R5 spacer

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK0_023

Plasmid

#123938

Purpose

Encodes the spacer TU with connectors L5 R6 as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

TUL5R6 spacer

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK0_024

Plasmid

#123939

Purpose

Encodes the spacer TU with connectors L6 R7 as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

TUL6R7 spacer

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK0_025

Plasmid

#123940

Purpose

Encodes the spacer TU with connectors L7 R8 as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

TUL7R8 spacer

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK0_026

Plasmid

#123941

Purpose

Encodes the spacer TU with connectors L8 RE as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

TUL8RE spacer

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK1_025

Plasmid

#123687

Purpose

Encodes ConL3 and human D1 insulator as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector L3-D1 insulator

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK1_026

Plasmid

#123688

Purpose

Encodes ConL4 and human E1 insulator as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector L4-E1 insulator

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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HOXD12 (murine) HIS-tag pET

Plasmid

#8553

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

Insert

HOXD12 (Hoxd12 Mouse)

Tags

His and T7

Expression

Bacterial

Mutation

Fusion sequence is: ggatccATG

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only