We narrowed to 88,257 results for: Mal

1. LITE2.0 pAAV_hSyn_NLS(alpha-imp)-CRY2PHR-NLS-VP64_2A_GFP_WPRE_bGHpA

   Plasmid

   #47455

   Purpose

   LITE2.0 CRY2PHR-VP64. Changes from LITE1.0: N-terminal alpha-importin nuclear localization sequence. Synapsin promoter for neuronal expression.

   Depositor

   Feng Zhang

   Article

   Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

   Insert

   NLS(alpha-imp)-CRY2PHR-NLS-VP64
   

   Use

   AAV

   Tags

   2A_GFP

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn human synapsin (mammalian neuronal expressio…

   Available Since

   Oct. 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA_B2AR-no-pep

   Plasmid

   #47439

   Purpose

   control FRET sensor for beta2-adrenergic receptor

   Depositor

   Sivaraj Sivaramakrishnan

   Article

   Malik et al J Biol Chem. 2013 Jun 14;288(24):17167-7

   Insert

   beta2-adrenergic receptor (ADRB2 Human)
   

   Use

   Tags

   10 nm ER/K helix, HA, mCerulean, mCitrine, and no…

   Expression

   Mammalian

   Mutation

   Promoter

   pCMV

   Available Since

   Sept. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

3. pTE4254

   Plasmid

   #89050

   Purpose

   Expresses St chimeric gRNA and human codon-optimized StCas9

   Depositor

   Ervin Welker

   Article

   Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.

   Inserts

   St chimeric gRNA
   hStCas9
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and human U6

   Available Since

   April 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-HCMVgO-sfGFP (Merlin)

   Plasmid

   #136452

   Purpose

   Mammalian expression plasmid for HCMV glycoprotein O (Merlin strain) fused to superfolder GFP

   Depositor

   Erik Procko

   Article

   Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

   Insert

   glycoprotein O (UL74 Human betaherpesvirus 5)
   

   Use

   Tags

   Gly/Ser-rich linker (GGSGGSGSGG) (includes BamHI …

   Expression

   Mammalian

   Mutation

   Codon-optimized for human cell expression.

   Promoter

   CMV

   Available Since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJZC25

   Plasmid

   #62328

   Purpose

   sgRNA + 1x MS2 with MCP-VP64 effector for mammalian cells

   Depositor

   Wendell Lim, Stanley Qi

   Article

   Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

   Inserts

   sgRNA + 1x MS2 binding module
   MCP-VP64
   

   Use

   Lentiviral

   Tags

   VP64

   Expression

   Mammalian

   Mutation

   Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA

   Promoter

   CMV and U6

   Available Since

   April 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLeo-A-a2-GEMS(JAK-STAT)

   Plasmid

   #209110

   Purpose

   Transient mammalian expression of the CC functionalized GEMS receptor A-a2-GEMS (JAK-STAT)

   Depositor

   Tom de Greef

   Article

   Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

   Insert

   A-a2-GEMS(JAK-STAT)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. Flag- mCry1ER-pBABEpuro

   Plasmid

   #61429

   Purpose

   Express mCRY1-ER* fusion protein

   Depositor

   Aziz Sancar

   Article

   Ye et al Genes Dev. 2014 Sep 15;28(18):1989-98. d

   Insert

   mCry1 (Cry1 Mouse)
   

   Use

   Retroviral

   Tags

   Flag and mouse estrogen receptor ligand binding d…

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

8. attB53_SNCB+_attB53

   Plasmid

   #183614

   Purpose

   RMCE vector to shuttle puromycin resistance, anti-GFP SynNotch receptor, tagBFP and TRE-mCherry cassettes into attP50-flanked landing pad (Addgene 183609). All cassettes on sense (+) strand.

   Depositor

   Sally Lowell

   Article

   Malaguti et al Development. 2022 May 26. pii: 275525. d

   Inserts

   LaG17 GFP nanobody SynNotch tTA
   TRE-mCherry-SV40pA
   tagBFP
   

   Use

   Rmce

   Tags

   Myc and human CD8a signal sequence

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA_B2AR-q-pep

   Plasmid

   #47437

   Purpose

   FRET sensor for beta2-adrenergic receptor

   Depositor

   Sivaraj Sivaramakrishnan

   Article

   Malik et al J Biol Chem. 2013 Jun 14;288(24):17167-7

   Insert

   beta2-adrenergic receptor (ADRB2 Human)
   

   Use

   Tags

   10 nm ER/K helix, HA, mCerulean, mCitrine, and q-…

   Expression

   Mammalian

   Mutation

   Promoter

   pCMV

   Available Since

   Sept. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

10. PD-1-miSFIT-12-C, 21-G

    Plasmid

    #124683

    Purpose

    Tuning expression of PD-1

    Depositor

    Tudor Fulga

    Article

    Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

    Insert

    PD-1 (PDCD1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    minimal CMV

    Available Since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-HCMVgH-8his (TB40/E)

    Plasmid

    #136445

    Purpose

    Mammalian expression plasmid for HCMV glycoprotein H (TB40/E strain) extracellular domain with 8his purification tag

    Depositor

    Erik Procko

    Article

    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

    Insert

    glycoprotein H (UL75 Human betaherpesvirus 5)
    

    Use

    Tags

    Short GGSG linker and 8his tag

    Expression

    Mammalian

    Mutation

    Extracellular domain (a.a. 1-717). Codon-optimize…

    Promoter

    CMV

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJZC32

    Plasmid

    #62327

    Purpose

    sgRNA (no RNA aptamer addition) with MCP-VP64 effector for mammalian cells

    Depositor

    Wendell Lim, Stanley Qi

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Inserts

    sgRNA
    MCP-VP64
    

    Use

    Lentiviral

    Tags

    VP64

    Expression

    Mammalian

    Mutation

    Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA

    Promoter

    CMV and U6

    Available Since

    April 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. YFP-Stim1-ΔCTD

    Plasmid

    #186621

    Purpose

    Encodes Stim1 with C-terminal domain deletion fused to YFP

    Depositor

    Gia Voeltz

    Article

    Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

    Insert

    Stromal Interaction Molecule 1 (STIM1 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Deletion of C-terminal domain 601 to 685

    Promoter

    CMV

    Available Since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. EF1a_ASCL5_P2A_Hygro_Barcode

    Plasmid

    #120430

    Purpose

    Barcoded lentiviral vector to express ASCL5 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    ASCL5 (ASCL5 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAS_4xEcoLeuT(CUA)_EF1_AnapRS

    Plasmid

    #174894

    Purpose

    AnapRS expression for amber suppression in mammalian cells; transient or piggy bac mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    AnapRS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    L38F, M40G, L41P, Y499V, Y500L, Y527A, H537E, L53…

    Promoter

    EF1

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-HCMVgO-8his (TB40/E)

    Plasmid

    #136453

    Purpose

    Mammalian expression plasmid for HCMV glycoprotein O (TB40/E strain) with an 8his tag

    Depositor

    Erik Procko

    Article

    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

    Insert

    envelope glycoprotein O (UL74 Human betaherpesvirus 5)
    

    Use

    Tags

    Linker and 8his tag (GGSGHHHHHHHH, includes BamHI…

    Expression

    Mammalian

    Mutation

    Codon-optimized for human cell expression.

    Promoter

    CMV

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3-HCMVgO-8his (Merlin)

    Plasmid

    #136454

    Purpose

    Mammalian expression plasmid for HCMV glycoprotein O (Merlin strain) with an 8his tag

    Depositor

    Erik Procko

    Article

    Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

    Insert

    glycoprotein O (UL74 Human betaherpesvirus 5)
    

    Use

    Tags

    Linker and 8his tag (GGSGHHHHHHHH, includes BamHI…

    Expression

    Mammalian

    Mutation

    Codon-optimized for human cell expression.

    Promoter

    CMV

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAG GST DEPDC5

    Plasmid

    #164983

    Purpose

    DEPDC5 expressing vector for mammalian cells

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Science. 2019 Nov 22;366(6468):971-977.

    Insert

    DEPDC5 (DEPDC5 Human)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    March 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEBG-IFNAR2(265-515)

    Plasmid

    #12437

    Depositor

    Dong-Er Zhang

    Article

    Malakhova et al EMBO J. 2006 Jun 7. 25(11):2358-67.

    Insert

    IFNAR2 a.a. 265-515 (IFNAR2 Human)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    pEBG vector is from Bruce Mayer, may be different…

    Promoter

    Available Since

    Jan. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

20. pEN_TT JunD-HA

    Plasmid

    #58498

    Purpose

    Gateway entry vector for an inducible HA-tagged mouse JunD

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

    Insert

    JunD (Jund Mouse)
    

    Use

    Entry vector

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 3, 2014

    Availability

    Academic Institutions and Nonprofits only