We narrowed to 10,382 results for: plasmids 101

1. HIS-HA-CMYC P53

   Plasmid

   #233738

   Purpose

   expresses HIS-HA-CMYC triple tagged P53 for pulldown assay

   Depositor

   Albert Keung

   Article

   Han et al Methods. 2025 Mar;235:92-99. doi: 10.101

   Insert

   HIS-HA-CMYC-p53 (TP53 Human)
   

   Use

   Tags

   HIS-HA-CMYC

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. scFv-GCN4-DNMT3a-DNMT3l

   Plasmid

   #154140

   Purpose

   Expresses the scFv-GCN4-DNMT3a-DNMT3l fusion protein (more details are shown in the vectro map) for targeted DNA methylation. Should be used in a combination with the dCas9-SunTag systems.

   Depositor

   Albert Jeltsch

   Article

   Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i

   Insert

   scFv-GCN4, DNMT3a (catalytic domain), DNMT3l (C-terminal part), sfGFP
   

   Use

   Tags

   HA and sfGFP

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available Since

   Oct. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDDGFP-Leu2d-GgKDELR2

   Plasmid

   #123618

   Purpose

   Expresses WT GgKDELR2 in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

   Depositor

   Simon Newstead

   Article

   Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

   Insert

   KDELR2 (KDELR2 Chicken)
   

   Use

   Tags

   TEV-GFP-His

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. GB1-PRD(703-800)-Strep

   Plasmid

   #141344

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-800)-Strep.

   Depositor

   Lalit Deshmukh

   Article

   Elias et al Proc Natl Acad Sci U S A. 2020 Sep 11. p

   Insert

   GB1-His6-TEV-ALIX(703-800)-Strep
   

   Use

   Tags

   GB1-His6-TEV and Strep-tag (WSHPQFEK)

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV_K700E_hSF3B1

   Plasmid

   #176591

   Purpose

   Adenoviral construct to change K to E at 700th amino acid (K700E) in human SF3B1

   Depositor

   Manoj Pillai

   Article

   Boddu et al Commun Biol. 2021 Oct 13;4(1):1184. doi:

   Insert

   SF3B1 (SF3B1 Human)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCRISPR_RAF1_94

   Plasmid

   #111826

   Purpose

   Knockout Raf1

   Depositor

   Steve Shih

   Article

   Sinha et al Lab Chip. 2018 Jul 24;18(15):2300-2312.

   Insert

   gRNA targeting RAF1 94-114 (RAF1 Human)
   

   Use

   CRISPR

   Tags

   mCherry

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-Syn-FLEX-rc [ChromeQ-GFP]

   Plasmid

   #153540

   Purpose

   AAV-mediated expression of ChromeQ-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) manner.

   Depositor

   Edward Boyden

   Article

   Cho et al J Biol Chem. 2019 Jan 4. pii: RA118.0069

   Insert

   ChromeQ-GFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Syn

   Available Since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-Syn-FLEX-rc [ChromeT-GFP]

   Plasmid

   #153539

   Purpose

   AAV-mediated expression of ChromeT-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) manner.

   Depositor

   Edward Boyden

   Article

   Cho et al J Biol Chem. 2019 Jan 4. pii: RA118.0069

   Insert

   ChromeT-GFP
   

   Use

   AAV

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Syn

   Available Since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. psiCHECK2-let-7 8x

   Plasmid

   #20931

   Depositor

   Yukihide Tomari

   Article

   Iwasaki et al Mol Cell. 2009 Apr 10;34(1):58-67. doi:

   Insert

   8x let-7 target sites
   

   Use

   Luciferase

   Tags

   Expression

   Insect and Mammalian

   Mutation

   Promoter

   Available Since

   June 19, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAN1717

    Plasmid

    #74696

    Purpose

    RPU standard plasmid

    Depositor

    Christopher Voigt

    Article

    Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

    Insert

    Insulated J23101-YFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMS2-1

    Plasmid

    #220627

    Purpose

    Plasmid used to place the RNA sequence of interest next to two MS2 coat protein recognition sites.

    Depositor

    Marvin Wickens

    Article

    Bernstein et al Methods. 2002 Feb;26(2):123-41. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. paavCAG-Jx

    Plasmid

    #51905

    Purpose

    Cre-dependent On/Off aav vector

    Depositor

    Jinhyun Kim

    Article

    Druckmann et al Neuron. 2014 Feb 5;81(3):629-40. doi: 10

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCM5.52

    Plasmid

    #19381

    Depositor

    Geraldine Seydoux

    Article

    Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

    Insert

    him-3 3'utr in pDONRP2RP3 (him-3 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    Oct. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCM3.23

    Plasmid

    #21389

    Depositor

    Geraldine Seydoux

    Article

    Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

    Insert

    gld-1 ORF+3' in pDONR201 (gld-1 Nematode)
    

    Use

    Gateway

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCM5.35

    Plasmid

    #19395

    Depositor

    Geraldine Seydoux

    Article

    Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

    Insert

    nos-3 3'utr in pDONRP2RP3 (nos-3 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    Nov. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCM5.43

    Plasmid

    #19391

    Depositor

    Geraldine Seydoux

    Article

    Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

    Insert

    daz-1 3'utr in pDONRP2RP3 (daz-1 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    Nov. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDR3.13

    Plasmid

    #19366

    Depositor

    Geraldine Seydoux

    Article

    Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

    Insert

    him-3 ORF+3' in pDONR201 (him-3 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    Nov. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCM3.29

    Plasmid

    #19402

    Depositor

    Geraldine Seydoux

    Article

    Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

    Insert

    lip-1 ORF+3' in pDONR201 (lip-1 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    Nov. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCM3.32

    Plasmid

    #19403

    Depositor

    Geraldine Seydoux

    Article

    Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

    Insert

    nos-3 ORF+3' in pDONR201 (nos-3 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    Nov. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-hSyn-R-iLACCO1.2

    Plasmid

    #208101

    Purpose

    Biosensor for intracellular L-lactate

    Depositor

    Robert Campbell, Marie-Eve Paquet

    Article

    Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

    Insert

    R-diLACCO1
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge