We narrowed to 29,111 results for: PLE;

1. pXWJ109AsAmp30E

   Plasmid

   #123144

   Purpose

   Arsenic sensor with an optimized HrpRS-based amplifier. pSB3K3 carrying J109-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-t

   Depositor

   Baojun Wang

   Article

   Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

   Insert

   J109-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-t
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J109, ParsR, PhrpLE

   Available since

   May 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMXSIP-GPR35-mCherry

   Plasmid

   #204711

   Purpose

   expresses GPR35-mCherry in mammalian cells

   Depositor

   William Kaelin

   Article

   Wyant et al Science. 2022 Aug 5;377(6606):621-629. d

   Insert

   G protein-coupled receptor 35 (GPR35 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSR02

   Plasmid

   #69149

   Purpose

   Read-out loxP mCherry to GFP switch for integration on CxTi10816, Mos chr IV

   Depositor

   Sander van den Heuvel

   Article

   Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

   Inserts

   mCherry
   eGFP
   

   Use

   Cre/Lox; Mossci

   Tags

   Expression

   Worm

   Mutation

   codon-optimzed index 1.0

   Promoter

   rps-27

   Available since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJRoC30_VP5H

   Plasmid

   #188061

   Purpose

   PROSS-designed versatile peroxidase 5H

   Depositor

   Sarel Fleishman

   Article

   Barber-Zucker et al J Am Chem Soc. 2022 Mar 2;144(8):3564-35

   Insert

   PROSS-designed versatile peroxidase from Pleurotus ostreatus
   

   Use

   Tags

   S. cerevisiae native α-factor prepro-leader signa…

   Expression

   Yeast

   Mutation

   43 PROSS mutations, described in publication

   Promoter

   Available since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAdSh.U6.gRNAS1

   Plasmid

   #58252

   Purpose

   U6 promoter-driven single guide RNA complementary to the human AAVS1 locus

   Depositor

   Manuel Goncalves

   Article

   Maggio et al Sci Rep. 2014 May 29;4:5105. doi: 10.103

   Insert

   U6.gRNAS1 cassette
   

   Use

   Adenoviral and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. ShadowG-SUMO1

   Plasmid

   #175239

   Purpose

   Bacterial expression and purification, conjugatable SUMO for SUMOylation

   Depositor

   Michael Rosen

   Article

   Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

   Insert

   SUMO1 (SUMO1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pXWJ109HgAmp30E

   Plasmid

   #123149

   Purpose

   Mercury sensor with an optimized HrpRS-based amplifier. pSB3K3 carrying J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30gfp-t

   Depositor

   Baojun Wang

   Article

   Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

   Insert

   J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30gfp-t
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J109, PmerT, PhrpLE

   Available since

   May 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRPL28(K55M) SpHIS5 (pNTI666)

   Plasmid

   #115436

   Purpose

   Expresses K55M RPL28 from HIS3 yeast plasmid

   Depositor

   Nicholas Ingolia

   Article

   McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

   Inserts

   RPL28 (RPL28 Budding Yeast)
   HIS5
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   K55M; MfeI site in intron

   Promoter

   Ashbya gospii TEF1 and endogenous

   Available since

   Oct. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pgRNA_hMECP2_promoter_No3

   Plasmid

   #194898

   Purpose

   Lentiviral construct with mCherry and Puro cassettes to express sgRNA-3 targeting the promoter of human MECP2

   Depositor

   Shawn Liu

   Article

   Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd

   Insert

   sgRNA targeting human MECP2 promoter No.3
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pgRNA_hMECP2_promoter_No9

    Plasmid

    #194903

    Purpose

    Lentiviral construct with mCherry and Puro cassettes to express sgRNA-9 targeting the promoter of human MECP2.

    Depositor

    Shawn Liu

    Article

    Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd

    Insert

    sgRNA targeting human MECP2 promoter No.9
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJZC42

    Plasmid

    #66564

    Purpose

    sgRNA + 1XPP7 with PCP-VP64 effector for mammalian cells, marked by mCherry

    Depositor

    Stanley Qi, Wendell Lim

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Inserts

    sgRNA + 1XPP7
    PCP-VP64 IRES mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. HA-MBP-TEVpCS-ADGRL3 CTF-FLAG

    Plasmid

    #217708

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRL3 (ADGRL3 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-841

    Promoter

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSLQ8703 pHR-R(TRE3G-Fluc-2XcrIFNg-SV40pA)-EF1a-sfGFP-T2A-rtta-WPRE

    Plasmid

    #140230

    Purpose

    Dox inducible expression of luciferase + crIFNg. Constitutive EF1a driven rtTA and GFP

    Depositor

    Stanley Qi

    Article

    Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

    Insert

    Luciferase + crIFNg (reverse orientation)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. HA-MBP-TEVpCS-ADGRG5 CTF-FLAG

    Plasmid

    #217703

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRG5 (ADGRG5 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-226

    Promoter

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. HA-MBP-TEVpCS-ADGRF4 CTF-FLAG

    Plasmid

    #217697

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRF4 (ADGRF4 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-384

    Promoter

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. RS18960_pMQ30_KO_Kan

    Plasmid

    #185393

    Purpose

    Non-replicating vector used to create markerless deletion of RR42_RS18960 in C. basilensis 4G11

    Depositor

    Adam Arkin

    Article

    Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

    Inserts

    RR42_RS18960 upstream flanking homology region
    RR42_RS18960 downstream flanking homology region
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTYF-sGFAP-LOx-IRES-tdTomato

    Plasmid

    #166919

    Purpose

    Express the bacterial lactate oxidase (LOx) enzyme in astrocytes

    Depositor

    Sergey Kasparov

    Article

    Vaccari Cardoso et al Glia. 2021 Jan 5. doi: 10.1002/glia.2396

    Insert

    Lactate oxidase
    

    Use

    Lentiviral

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    sGFAP

    Available since

    April 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCfB2803

    Plasmid

    #63646

    Purpose

    pCfB2803 is a vector for multiple integrations at sites sharing homology with Ty4Cons. The selective marker is Kl.LEU2. Additionally it contains a USER cassette.

    Depositor

    Jerome Maury, Irina Borodina

    Article

    Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    none

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. RS17060_pMQ30_KO_Kan

    Plasmid

    #185396

    Purpose

    Non-replicating vector used to create markerless deletion of RR42_RS17060 in C. basilensis 4G11

    Depositor

    Adam Arkin

    Article

    Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

    Inserts

    RR42_RS17060 upstream flanking homology region
    RR42_RS17060 downstream flanking homology region
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. RS17055_pMQ30_KO_Kan

    Plasmid

    #185398

    Purpose

    Non-replicating vector used to create markerless deletion of RR42_RS17055 in C. basilensis 4G11

    Depositor

    Adam Arkin

    Article

    Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

    Inserts

    RR42_RS17055 upstream flanking homology region
    RR42_RS17055 downstream flanking homology region
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge