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We narrowed to 27,982 results for: STI

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Showing: 3761 - 3780 of 27982 results

  1. pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA1a_(CJT90)

    Plasmid
    #226989
    Purpose
    pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 1a must be used with gRNA 2a or 2b
    Depositor
    Benjamin Kleinstiver
    Article
    Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.
    Insert
    SpCas9 gRNA 1a to create OPA1-del_exon5
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    226989-plasmid-map-sequence-id-453964

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  2. pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA2a_(CJT91)

    Plasmid
    #226991
    Purpose
    pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 2a must be used with gRNA 1a or 1b
    Depositor
    Benjamin Kleinstiver
    Article
    Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.
    Insert
    SpCas9 gRNA 2a to create OPA1-del_exon5
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    226991-plasmid-map-sequence-id-453954

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  5. pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA2b_(CJT93)

    Plasmid
    #226992
    Purpose
    pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 2b must be used with gRNA 1a or 1b
    Depositor
    Benjamin Kleinstiver
    Article
    Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.
    Insert
    SpCas9 gRNA 2b to create OPA1-del_exon5
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    226992-plasmid-map-sequence-id-451253

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  6. pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA1b_(CJT92)

    Plasmid
    #226990
    Purpose
    pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 1b must be used with gRNA 2a or 2b
    Depositor
    Benjamin Kleinstiver
    Article
    Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.
    Insert
    SpCas9 gRNA 1b to create OPA1-del_exon5
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    226990-plasmid-map-sequence-id-451250

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  8. SSH-pcDNA3.1-SARS-CoV-2-S-Lys1269Ala-His1271Ala

    Plasmid
    #218463
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 spike (1273 residues; Lys1269Ala and His1271Ala substitutions)
    Depositor
    Syed Saif Hasan
    Article
    Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:
    Insert
    SARS-CoV-2 spike protein (1273 residues) carrying Lys1269Ala and His1271Ala substitutions (S )
    Expression
    Mammalian
    Mutation
    Lys1269Ala and His1271Ala substitutions
    Available Since
    June 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    218463-plasmid-map-sequence-id-436199

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  9. pLX304_miRFP670nano-P2A-ERM-gCam6f

    Plasmid
    #214927
    Purpose
    Express miRFP670nano P2A ERM-gCaMP6f (fluorescent proteins and genetic calcium sensor, gCaMP6f for normalizing factor and sensing calcium ions at cytosol exposed to ERM, respectively)
    Depositor
    Xinnan Wang
    Article
    Bharat et al Cell Rep. 2023 Dec 26;42(12):113544. doi
    Insert
    miRFP670nano-P2A-ERM-gCam6f
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    CMVp
    Available Since
    April 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    214927-plasmid-map-sequence-id-429502

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  10. Polymerase expression - pCMV-ePhi29(+exo) (LM2995)

    Plasmid
    #208966
    Purpose
    Unfused ePhi29 DNA polymerase (+exo), expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    ePhi29(+exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    ePhi29(+exo)(M8R/V51A/M97T/G197D/E221K/Q497P/K512…
    Promoter
    CMV and T7
    Available Since
    Feb. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    208966-plasmid-map-sequence-id-422883

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  12. Recruited polymerase expression - pCMV-T7-ePhi29-CC(N5) (LM2719)

    Plasmid
    #208973
    Purpose
    Recruited ePhi29 DNA polymerase (-exo), with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    ePhi29-BPNLS-CC(N5)
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    ePhi29(-exo)(D169A;M8R/V51A/M97T/G197D/E221K/Q497…
    Promoter
    CMV and T7
    Available Since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208973-plasmid-map-sequence-id-415830

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  13. pPV-C1-crRNA(DMD#20_DMD#23)-EF1a-BA

    Plasmid
    #204620
    Purpose
    Expression vector of crRNA (DMD#20) targeting the dystrophin intron 44 just upstream of exon 45, and crRNA(DMD#23)targeting the dystrophin intron 55 just downstream of exon 55. Blasticidin selectable.
    Depositor
    Akitsu Hotta
    Article
    Kita et al Stem Cell Reports. 2023 Sep 12;18(9):175
    Insert
    crRNA#20 (targeting DMD intron44) and crRNA#23 (targeting DMD intron 55)
    Use
    CRISPR
    Promoter
    U6
    Available Since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    204620-plasmid-map-sequence-id-404750

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  14. pUt-mNF-GFP

    Plasmid
    #199220
    Purpose
    LPUtopia-7 matching RMCE donor plasmid with GFP Negative-Feedback circuit (mNF-GFP). Use BlastR for positive selection and HSV-TK for negative selection. 
    Depositor
    Gabor Balazsi
    Article
    Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d
    Insert
    hTetR::P2A::EGFP
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV D2ir promoter
    Available Since
    June 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    199220-plasmid-map-sequence-id-396466

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  15. LeGO-iBSD-5LO-d4

    Plasmid
    #182743
    Purpose
    Lentiviral expression vector for codon-optimized 5-Lipoxygenase (isoform delta 4) in mamallian cells with blasticidin S resistance (bsd).
    Depositor
    Dieter Steinhilber
    Article
    Kreiss et al Biochem Pharmacol. 2022 Jul 22:115187. d
    Insert
    5-Lipoxygenase isoform delta 4 (ALOX5 Human)
    Use
    Cre/Lox and Lentiviral
    Expression
    Mammalian
    Mutation
    codon optimized for expression in homo sapiens
    Promoter
    SFFV
    Available Since
    July 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    182743-plasmid-map-sequence-id-367810

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  16. LeGO-iBSD-5LO-d13

    Plasmid
    #182745
    Purpose
    Lentiviral expression vector for codon-optimized 5-Lipoxygenase (isoform delta 13) in mamallian cells with blasticidin S resistance (bsd).
    Depositor
    Dieter Steinhilber
    Article
    Kreiss et al Biochem Pharmacol. 2022 Jul 22:115187. d
    Insert
    5-Lipoxygenase isoform delta 13 (ALOX5 Human)
    Use
    Cre/Lox and Lentiviral
    Expression
    Mammalian
    Mutation
    codon optimized for expression in homo sapiens
    Available Since
    July 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    182745-plasmid-map-sequence-id-367797

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  19. 289aa ELL2

    Plasmid
    #127266
    Purpose
    For in vitro translation of shorter human ELL2 from Met1. Also contains Met133I, M1381I, M186I mutations.
    Depositor
    Christine Milcarek
    Article
    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi
    Insert
    NH2-ELL2 (Met133ILeu, M1381ILeu, M186ILeu) (ELL2 Human)
    Use
    In vitro translation
    Mutation
    Three Mets (133, 138, 186) to Ileu. Contains 289…
    Promoter
    T7
    Available Since
    July 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    127266-plasmid-map-sequence-id-250216

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Showing: 3761 - 3780 of 27982 results