We narrowed to 5,803 results for: SUP

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pEasyG3_hph

Plasmid

#184919

Purpose

Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_hph recombines in vivo with a PCR product from pEasyG3_mic.

Depositor

Jeferson Gross

Article

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

gRNA scaffold

Use

CRISPR

Expression

Bacterial

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pRSET a sfMatryoshCaMP6s-T78H

Plasmid

#100021

Purpose

Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference LSSmOrange nested within the reporting superfolder cpGFP-T78H

Depositor

Wolf Frommer

Article

Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

Insert

sfMatryoshCaMP6s

Tags

6x HIS tag and Xpress_EK tag

Expression

Bacterial

Mutation

cpEGFP replaced with superfolder cpGFP and T78H m…

Promoter

T7

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pBMN HA-ATG13

Plasmid

#186223

Purpose

For expression of ATG13 tagged with HA

Depositor

Michael Lazarou

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

ATG13 (ATG13 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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PGEX-6P1-hNF2-AR mutant

Plasmid

#107149

Purpose

bacterial expression

Depositor

Tina Izard

Article

Chinthalapudi et al Nat Commun. 2018 Apr 6;9(1):1338. doi: 1

Insert

neurofibromin 2 (NF2 Human)

Tags

GST

Expression

Bacterial

Mutation

A585W-R588K

Promoter

Ptac

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pSECC-sgLKB1-A

Plasmid

#138663

Purpose

Expresses a mouse LKB1-targeting sgRNA, Cas9, and Cre-recombinase

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgLKB1 mouse (Stk11 Mouse)

Use

Lentiviral

Promoter

hU6

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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RK5F-YAP8SA

Plasmid

#125008

Purpose

express in mammalian cell

Depositor

Ruey-Hwa Chen

Article

Tan et al Oncogene. 2019 Jul;38(28):5612-5626. doi

Insert

YAP8SA (YAP1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2022

Availability

Academic Institutions and Nonprofits only

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pFLAG-CMV2 MAML LHHLL

Plasmid

#87681

Purpose

Mammalian expression vector for Maml1 with LHHLL mutation

Depositor

Karl Munger

Article

Meyers et al PLoS Pathog. 2017 Jan 20;13(1):e1006171.

Insert

MAML1 (MAML1 Human)

Tags

Flag

Expression

Mammalian

Mutation

Mutate 1010,1011 from DD to HH

Promoter

CMV

Available Since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR CMVie-Lifeact-iRFP670 L1-R5

Plasmid

#84390

Purpose

Multisite Gateway entry vector for expression of iRFP670-tagged Lifeact in cells including neurons

Depositor

Ghassan Mouneimne

Article

Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

Insert

Lifeact

Use

Gateway

Tags

iRFP670

Expression

Mammalian

Promoter

CMV immediate early

Available Since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-Puro-SIK2

Plasmid

#138696

Purpose

Expresses a human SIK2-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgSIK2 human (SIK2 Human)

Use

Lentiviral

Promoter

hU6

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-Puro-SIK3

Plasmid

#138697

Purpose

Expresses a human SIK3-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgSIK3 human (SIK3 Human)

Use

Lentiviral

Promoter

hU6

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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Frt-hspB6

Plasmid

#63097

Purpose

Expression of HSPB6 (small HSP) in mammalian cells

Depositor

Harm Kampinga

Article

Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

Insert

hspb6 (HSPB6 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 20, 2016

Availability

Academic Institutions and Nonprofits only

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Frt-hspB4

Plasmid

#63095

Purpose

Expression of HSPB4 (small HSP) in mammalian cells

Depositor

Harm Kampinga

Article

Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

Insert

hspb4 (CRYAA Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

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pDN-G1Tt

Plasmid

#44509

Depositor

Gabor Balazsi

Article

Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

Inserts

PGal1-D12 promoter (GAL1 Synthetic, Budding Yeast)
tetR

Use

Synthetic Biology; Expression regulator/reporter

Expression

Yeast

Mutation

Two tandem tet operators (2XtetO2) downstream of …

Promoter

PGal1-D12 promoter

Available Since

April 23, 2013

Availability

Academic Institutions and Nonprofits only

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496 pSG5L HA RB del651 (NAAIRS)

Plasmid

#10726

Depositor

William Sellers

Article

Sellers et al Genes Dev. 1998 Jan 1. 12(1):95-106.

Insert

RB del651 (RB1 Human)

Tags

HA

Expression

Mammalian

Mutation

residues 651 to 656 were replaced by NAAIRS, a fl…

Available Since

Sept. 27, 2005

Availability

Academic Institutions and Nonprofits only
500 pSG5L HA RB del685 (NAAIRS)

Plasmid

#10729

Depositor

William Sellers

Article

Sellers et al Genes Dev. 1998 Jan 1. 12(1):95-106.

Insert

RB del685 (RB1 Human)

Tags

HA

Expression

Mammalian

Mutation

residues 685 to 690 were replaced by NAAIRS, a fl…

Available Since

Sept. 27, 2005

Availability

Academic Institutions and Nonprofits only
SIN40C.TRE.dTomato.miR-125b-2.PGK.sfGFP.P2A.Tet3G

Plasmid

#169315

Purpose

Doxycycline-inducible expression of miR-125b-2 with dTomato as reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

miR-125b-2 (MIR125B2 Human)

Use

Lentiviral

Tags

dTomato

Promoter

TRE

Available Since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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LRG-2.1T-neo-sgTAF12(human)#4.4

Plasmid

#105590

Purpose

lentivirally express gRNA targeting human TAF12 HFD with GFP marker and neo resistance

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

gRNA targeting human TAF12 HFD

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

March 5, 2018

Availability

Academic Institutions and Nonprofits only

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pEasyG2_zeo

Plasmid

#184913

Purpose

Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_zeo recombines in vivo with a PCR product from pEasyG2_mic.

Depositor

Jeferson Gross

Article

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

gRNA scaffold

Use

CRISPR

Expression

Bacterial

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pEasyG2_nat

Plasmid

#184914

Purpose

Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_nat recombines in vivo with a PCR product from pEasyG2_mic.

Depositor

Jeferson Gross

Article

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

gRNA scaffold

Use

CRISPR

Expression

Bacterial

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEasyG3_zeo

Plasmid

#184917

Purpose

Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_zeo recombines in vivo with a PCR product from pEasyG3_mic.

Depositor

Jeferson Gross

Article

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

gRNA scaffold

Use

CRISPR

Expression

Bacterial

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge