We narrowed to 10,222 results for: ada

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pHLmMBP-1

Plasmid

#72343

Purpose

Mammalian expression of secreted mMBP-fused proteins with C-terminal 6His-tag/Strep-tag II/HA-tag

Depositor

Luca Jovine

Article

Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii

Type

Empty backbone

Tags

6His-tag, HA-tag, Strep-tag II, and mMBP

Expression

Mammalian

Promoter

CMV enhancer + chicken beta-actin promoter

Available Since

Feb. 17, 2016

Availability

Academic Institutions and Nonprofits only

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1105-Desmoglein2-FLAG-eGFP

Plasmid

#36989

Depositor

Kathleen Green

Article

Klessner et al Mol Biol Cell. 2009 Jan;20(1):328-37. Ep

Insert

Dsg2 (DSG2 Human)

Tags

FLAG and eGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 5, 2012

Availability

Academic Institutions and Nonprofits only

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pEGFP-UBE4B

Plasmid

#113499

Purpose

Expression of human UBE4B with N-terminal GFP tag

Depositor

Hemmo Meyer

Article

Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

Insert

UBE4B (UBE4B Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pPOTv7-hyg-AtAID:HA (p5339)

Plasmid

#239363

Purpose

pPOTv7 based plasmid template for addition of HA-epitope tagged Rice Auxin Inducible Degradation (AID) domain to genes in trypanosomes with selection using hygromycin

Depositor

Mark Carrington

Article

Alexandra et al bioRxiv 2025.06.17.660065

Insert

Arabidopsis Auxin Inducible Degradation domain

Use

Bacterial

Tags

1 x HA epitope

Promoter

none

Available Since

July 16, 2025

Availability

Academic Institutions and Nonprofits only

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pGEMHE-mEGFP-mTrim21

Plasmid

#105519

Purpose

T7 promotor drives in vitro transcription of mEGFP-tagged wildtype mouse TRIM21 mRNA

Depositor

Melina Schuh

Article

Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

Insert

TRIM21 (Trim21 Mouse)

Use

In vitro transcription

Tags

mEGFP

Promoter

T7

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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7Gli:GFP

Plasmid

#110494

Purpose

reporter of Hedgehog signaling activity

Depositor

Michael Lewis

Article

Li et al PLoS Genet. 2016 May 19;12(5):e1006054.

Insert

7 repeats of the Gli binding site GAACACCCA

Use

Lentiviral

Expression

Mammalian

Promoter

7 repeats of Gli biding site

Available Since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

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pX330-PITCh-H2BC11

Plasmid

#207755

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of H2BC11 for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting C-terminus of H2BC11 (H2BC11 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Dec. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pCAGGS-6011nes

Plasmid

#108652

Purpose

An ERK biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

Depositor

Michiyuki Matsuda

Article

Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

Insert

hyBRET-ERK-EV

Tags

XFP

Expression

Mammalian

Promoter

pCAGGS

Available Since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG-USP5

Plasmid

#61742

Purpose

express N-terminally FLAG tagged human USP5

Depositor

Yihong Ye

Article

Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

Insert

USP5 (USP5 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2015

Availability

Academic Institutions and Nonprofits only

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qTAG-AAVS1-PGK-Blast

Plasmid

#227270

Purpose

Empty AAVS1 targeting donor for the insertion of Blast and a medium strength PGK promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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hIRE1a K599A

Plasmid

#20745

Depositor

Fumihiko Urano

Article

Lipson et al PLoS ONE. 2008 . 3(2):e1648.

Insert

IRE1 (ERN1 Human)

Use

Lentiviral; Destination vector

Expression

Mammalian

Mutation

K599A, dominant negative kinase mutant

Available Since

April 14, 2009

Availability

Academic Institutions and Nonprofits only
EP-Pol vector

Plasmid

#131686

Purpose

A lentiviral vector that constitutively expresses an error-prone variant of the adenovirus DNA polymerase

Depositor

Matthew D. Shoulders

Article

Berman et al J Am Chem Soc. 2018 Dec 26;140(51):18093

Insert

Adenovirus DNA polymerase

Use

Lentiviral

Tags

HA

Mutation

F421Y, D827A

Available Since

May 7, 2020

Availability

Academic Institutions and Nonprofits only

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His-drebrin

Plasmid

#40362

Depositor

Phillip Gordon-Weeks

Article

Geraldo et al Nat Cell Biol. 2008 Oct;10(10):1181-9. E

Insert

drebrin E2 (DBN1 Human)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Dec. 19, 2012

Availability

Academic Institutions and Nonprofits only
pTRE2-Bla(HA–RILP-FLAG)

Plasmid

#102424

Purpose

Dual tagged RILP for expression in mammalian cells.

Depositor

Steven Weinman

Article

Wozniak et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

Insert

Rab interacting lysosomal protein (RILP Human)

Tags

FLAG and HA

Expression

Mammalian

Promoter

Tet-Responsive

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pME18s-HA-hFIP200

Plasmid

#24303

Depositor

Noboru Mizushima

Article

Hosokawa et al Mol Biol Cell. 2009 Apr . 20(7):1981-91.

Insert

FIP200 (RB1CC1 Human)

Tags

3xHA

Expression

Mammalian

Available Since

June 24, 2010

Availability

Academic Institutions and Nonprofits only

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pSBbi_RP_3XNLS-MicroID2-FLAG

Plasmid

#188523

Purpose

Encodes the biotin proximity labeling enzyme MicroID2 with a 3X nuclear localization sequence (NLS) for expression and stable integration into mammalian cells

Depositor

James Londino

Article

Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.

Insert

3XNLS-MicroID2

Use

Transposon mediated gene integration

Tags

FLAG

Expression

Mammalian

Promoter

EF1a/RPBSA

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pMDJ10 - pEXP[Peft-3 | tagBFP2 (NLS) | tbb-2 UTR]

Plasmid

#159798

Purpose

Expression construct with ubiquitous expression to test fluorescence level on microscope

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP[Peft-3 | tagBFP2 (NLS) | tbb-2 UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP.N1-HDAC6.DC (catalytic deficient)

Plasmid

#36189

Depositor

Tso-Pang Yao

Article

Gao et al J Biol Chem. 2010 Apr 9. 285(15):11219-2

Insert

HDAC6.DC (HDAC6 Human)

Tags

EGFP

Expression

Mammalian

Mutation

H216/611A

Promoter

CMV

Available Since

April 19, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Barr2 flag

Plasmid

#14685

Depositor

Robert Lefkowitz

Article

Laporte et al Proc Natl Acad Sci U S A. 1999 Mar 30. 9

Insert

beta-arrestin 2 (Arrb2 Rat)

Tags

flag

Expression

Mammalian

Available Since

June 19, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3-hARFGAP2-HA

Plasmid

#206410

Purpose

Mammalian expression of hARFGAP2

Depositor

Kazuhisa Nakayama, Hye-Won Shin

Article

Saitoh et al J Biol Chem. 2009 May 15;284(20):13948-1

Insert

ARFGAP2 (ARFGAP2 Human)

Tags

HA

Expression

Mammalian

Available Since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

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