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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 11,800 results for: emb

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Showing: 3761 - 3780 of 11800 results

  2. pCENPTΔC-dCas9-3xGFP

    Plasmid
    #198325
    Purpose
    Expresses CENPTΔC-dCas9-3xGFP protein in mammalian cells. When coupled with a guide RNA against a high repeat target locus, this can be applied to seed ectopic kinetochores at the target locus
    Depositor
    Sarah McClelland
    Publication
    Tovini et al EMBO J. 2023 May 15;42(10):e111587. doi:
    Insert
    CENPTΔC (CENPT Human)
    Use
    Lentiviral
    Tags
    EGFP x 3 and dCas9
    Expression
    Mammalian
    Mutation
    truncation - encodes only amino acids 1-375
    Promoter
    CMV-TetO
    Available since
    April 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    198325-plasmid-map-sequence-id-390299

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  3. pCS2+/N-Halo

    Plasmid
    #184417
    Purpose
    vector backbone to clone insert downstream of Halo tag (Halo tag codon optimized for Xenopus), for mRNA synthesis
    Depositor
    Ann Miller
    Publication
    Dudley et al STAR Protoc. 2022 Aug 18;3(3):101622. do
    Type
    Empty backbone
    Use
    For mrna synthesis
    Tags
    Halo-tag
    Mutation
    Codon optimized for expression in Xenopus laevis …
    Available since
    Aug. 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    184417-plasmid-map-sequence-id-362159

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  4. pCAX-EGFP-FBP17

    Plasmid
    #178365
    Purpose
    Expresses FBP17 c-terminal labeled with EGFP in mammalian cells
    Depositor
    Erik Dent
    Publication
    Taylor et al Life Sci Alliance. 2019 Jun 3;2(3). pii:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Formin Binding Protein 17 (FNBP1 Human)
    Expression
    Mammalian
    Promoter
    Chicken Beta Actin
    Available since
    March 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    178365-plasmid-map-sequence-id-351834

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  10. Halo-Erk2 donor

    Plasmid
    #113104
    Purpose
    Donor plasmid for knock-in HaloTag fusing to the N-terminal of mouse Erk2 coding sequence
    Depositor
    Janet Rossant
    Publication
    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    N terminal Erk2 donor region including homology arms and HaloTag
    Use
    Synthetic Biology
    Available since
    Aug. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    113104-plasmid-map-sequence-id-217907

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  20. His-GFP-Rim2

    Plasmid
    #170869
    Purpose
    Mammalian expression of His-GFP-Rim2.
    Depositor
    Justin Taraska
    Publication
    Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Regulating synaptic membrane exocytosis protein 2 (Rim 2) (Rims2 Rat)
    Tags
    (His)6 and EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    170869-plasmid-map-sequence-id-335145

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Showing: 3761 - 3780 of 11800 results