Addgene

We narrowed to 2,950 results for: ER

Showing: 721 - 760 of 2950 results

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FLAG-Sec31a S1163A #13#904

Plasmid

#42118

Depositor

Ayano Satoh

Article

Koreishi et al PLoS One. 2013;8(1):e54382. doi: 10.1371

Insert

Sec31a (SEC31A Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

1163A

Promoter

CMV

Available since

Oct. 4, 2013

Availability

Academic Institutions and Nonprofits only
FLAG-Sec31a W985A #790

Plasmid

#42122

Depositor

Ayano Satoh

Article

Koreishi et al PLoS One. 2013;8(1):e54382. doi: 10.1371

Insert

Sec31a (SEC31A Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

G742R and W985A NOTE: the mutation is actually W…

Promoter

CMV

Available since

March 12, 2013

Availability

Academic Institutions and Nonprofits only
FLAG-Sec31a-4SA #33#630

Plasmid

#42114

Depositor

Ayano Satoh

Article

Koreishi et al PLoS One. 2013;8(1):e54382. doi: 10.1371

Insert

Sec31a (SEC31A Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

527A/779A/1163A/1165A (4SA)

Promoter

CMV

Available since

Feb. 20, 2013

Availability

Academic Institutions and Nonprofits only
FLAG-Sec31a-4SD #36#631

Plasmid

#42115

Depositor

Ayano Satoh

Article

Koreishi et al PLoS One. 2013;8(1):e54382. doi: 10.1371

Insert

Sec31a (SEC31A Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

527D/779D/1163D/1165D (4SD)

Promoter

CMV

Available since

Feb. 20, 2013

Availability

Academic Institutions and Nonprofits only
FLAG-Sec31a S527/799A #27#905

Plasmid

#42119

Depositor

Ayano Satoh

Article

Koreishi et al PLoS One. 2013;8(1):e54382. doi: 10.1371

Insert

Sec31a (SEC31A Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

S527A S799A

Promoter

CMV

Available since

Feb. 20, 2013

Availability

Academic Institutions and Nonprofits only
pTRE-8XGTIIC-DsRED-DD

Plasmid

#115798

Purpose

lentiviral, trimethoprim (TMP) regulated YAP promoter activity reporter

Depositor

Joan Massague

Article

Er et al Nat Cell Biol. 2018 Aug;20(8):966-978. d

Insert

YAP responsive promoter (YAP1 Human)

Use

Lentiviral

Tags

DsRED-DHFR

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-20nmEML-mRFP

Plasmid

#231178

Purpose

Stabilizes the ER-mitochondrial distance at 20 nm; tagged with mRFP

Depositor

Dmitry Lim

Article

Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

Insert

20nmEML-mRFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-30nmEML-mRFP

Plasmid

#231179

Purpose

Stabilizes the ER-mitochondrial distance at 30 nm; tagged with mRFP

Depositor

Dmitry Lim

Article

Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

Insert

30nmEML-mRFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-15nmEML-mRFP

Plasmid

#231177

Purpose

Stabilizes the ER-mitochondrial distance at 15 nm; tagged with mRFP

Depositor

Dmitry Lim

Article

Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

Insert

15nmEML-mRFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
mScarlet-Rab7

Plasmid

#169068

Purpose

Express human Rab7a in mammalian cells

Depositor

Gia Voeltz

Article

Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

Insert

Rab7a (RAB7A Human)

Use

Tags

mScarlet

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pEM705 Gsi (Ser)

Plasmid

#109352

Purpose

GsX chimera with Gαi C-terminus (Pertussis toxin insensitive mutant)

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

Gsi chimera

Use

Tags

None

Expression

Mammalian

Mutation

Changed Cys at position 391 to Serine (Pertussis …

Promoter

CAG

Available since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pLX304-Flag-APEX2-NES

Plasmid

#92158

Purpose

Cytosolic APEX2 construct

Depositor

Alice Ting

Article

Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:

Insert

Flag-APEX2-NES

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pBXNH3CA_MBP

Plasmid

#132700

Purpose

expresses Avi-tagged MBP in E.coli, to use as positive control in ELISA

Depositor

Markus Seeger

Article

Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

Insert

Maltose binding Protein

Use

Tags

Avi and His

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pTP396

Plasmid

#149336

Purpose

Bacterial expression of His14-Biotin_acceptor_peptide-SUMOEu1-anti-GFP_nanobody

Depositor

Rebecca Voorhees

Article

Pleiner et al Science. 2020 May 21. pii: science.abb50

Insert

anti-GFP nanobody

Use

Tags

Biotin acceptor peptide (Avi-tag), His14, and SUM…

Expression

Bacterial

Mutation

Promoter

Available since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

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pEF Synaptophysin-mRuby

Plasmid

#188980

Purpose

Synaptic buton marker

Depositor

Edwin Chapman

Article

Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

Insert

Synaptophysin (Syp Synthetic, Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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PB_rtTA_BsmBI

Plasmid

#126028

Purpose

PiggyBac cargo vector expressing rtTA, contains BsmBI sites for sgRNA cloning

Depositor

Mauro Calabrese

Article

Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

Type

Empty backbone

Use

CRISPR; Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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mScarlet-EEA1

Plasmid

#169067

Purpose

Express human EEA1 in mammalian cells

Depositor

Gia Voeltz

Article

Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

Insert

EEA1 (EEA1 Human)

Use

Tags

mScarlet

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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LgBiT-ST

Plasmid

#207633

Purpose

A plasmid encoding LgBiT fused to SpyTag

Depositor

Cole DeForest

Article

Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

Insert

LgBiT-SpyTag

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pMPMA3dPlac-PprgH-GFPLVA

Plasmid

#23340

Depositor

Olivia Steele-Mortimer

Article

Ibarra et al Microbiology. 2009 Dec 24. ():.

Insert

GFP(LVA)

Use

Tags

GFP(LVA)

Expression

Bacterial

Mutation

Promoter

Available since

April 27, 2010

Availability

Academic Institutions and Nonprofits only
VPS13D^EGFP

Plasmid

#174109

Purpose

The insert is a codon optimized DNA sequence encoding VPS13D with an internal EGFP.

Depositor

Pietro De Camilli

Article

Guillen-Samander et al J Cell Biol. 2021 May 3;220(5). pii: 212

Insert

Codon optimized VPS13D

Use

Tags

EGFP

Expression

Mammalian

Mutation

With an internal EGFP tag in aminoacid position 1…

Promoter

CMV

Available since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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Rtn3L-mNeonGreen

Plasmid

#169034

Purpose

Express human Rtn3L in mammalian cells

Depositor

Gia Voeltz

Article

Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

Insert

Rtn3L (RTN3 Human)

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG mito-RCaMP1h

Plasmid

#105013

Purpose

mitochondrial calcium sensor

Depositor

Franck Polleux

Article

Hirabayashi et al Science. 2017 Nov 3;358(6363):623-630. d

Insert

RCaMP1h

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pMRX-INU-FLAG-FAM134B

Plasmid

#128260

Purpose

Expresses FAM134B in mammalian cells

Depositor

Noboru Mizushima

Article

Chino et al Mol Cell. 2019 Apr 11. pii: S1097-2765(1

Insert

FAM134B (RETREG1 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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pQCXIZ-turq-3xFLAG-TREX1

Plasmid

#164232

Purpose

retroviral plasmid for turq-3xFLAG-TREX1

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

TREX1 (TREX1 Human)

Use

Retroviral

Tags

FLAG and mturquoise

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 1, 2021

Availability

Academic Institutions and Nonprofits only

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pCAT001

Plasmid

#171061

Purpose

Plasmid for expressing HIV-1 envelope glycoprotein

Depositor

Jennifer Doudna

Article

Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

Insert

HIV-1 Env

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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pFsynW SYP-GFP

Plasmid

#197283

Purpose

Encodes for viral expression of full-length wild type synaptophysin (SYP). Visualized by a C-terminal GFP.

Depositor

Edwin Chapman

Article

Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

Insert

SYP and GFP (Syp Rat)

Use

Lentiviral

Tags

1D4 epitope

Expression

Mutation

N/A

Promoter

hSyn (human synapsin I promoter)

Available since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMPMA3dPlac-null-GFPLVA

Plasmid

#23338

Depositor

Olivia Steele-Mortimer

Article

Ibarra et al Microbiology. 2009 Dec 24. ():.

Insert

GFP(LVA)

Use

Tags

GFP(LVA)

Expression

Bacterial

Mutation

Promoter

Available since

April 27, 2010

Availability

Academic Institutions and Nonprofits only
pTol2-Huc-Positron-ST

Plasmid

#129265

Purpose

pan neuronal expression of soma-localized Positron in zebrafish

Depositor

Eric Schreiter

Article

Abdelfattah et al Nat Commun. 2020 Jul 10;11(1):3444. doi:

Insert

Positron-ST

Use

Zebrafish expression

Tags

Expression

Mutation

D92N, E199V

Promoter

Available since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG cyto-RCaMP1h

Plasmid

#105014

Purpose

cytosolic calcium sensor

Depositor

Franck Polleux

Article

Hirabayashi et al Science. 2017 Nov 3;358(6363):623-630. d

Insert

RCaMP1h

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pMRX-INU-SEC62-FLAG

Plasmid

#128263

Purpose

Expresses SEC62 in mammalian cells

Depositor

Noboru Mizushima

Article

Chino et al Mol Cell. 2019 Apr 11. pii: S1097-2765(1

Insert

SEC62 (SEC62 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Rtn2b-GFP

Plasmid

#186599

Purpose

Encodes human full-length Reticulon 2 isoform B fused to GFP

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Human Reticulon 2 isoform B (RTN2 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
VPS13D^mScarlet

Plasmid

#174110

Purpose

The insert is a codon optimized DNA sequence encoding VPS13D with an internal mScarlet protein.

Depositor

Pietro De Camilli

Article

Guillen-Samander et al J Cell Biol. 2021 May 3;220(5). pii: 212

Insert

Codon optimized VPS13D

Use

Tags

mScarlet

Expression

Mammalian

Mutation

With an internal mScarlet tag in aminoacid positi…

Promoter

CMV

Available since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pPW3756 : CMVd3-ERmembrane-HaloTag-KKMP

Plasmid

#185678

Purpose

Low-level transient expression of a single HaloTag protein with a C-terminal KKMP ER retention signal, targeted to the ER membrane.

Depositor

Peter Walter

Article

Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

Insert

ERmembrane-HaloTag-KKMP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Rtn2b-mCherry

Plasmid

#186600

Purpose

Encodes human full-length Reticulon 2 isoform B fused to mCherry

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Human Reticulon 2 isoform B (RTN2 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEM705 Gst (Ser)

Plasmid

#109354

Purpose

GsX chimera with Gαt C-terminus (Pertussis toxin insensitive mutant)

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

Gst chimera

Use

Tags

None

Expression

Mammalian

Mutation

Changed Cysteine at position 391 to Serine (Pertu…

Promoter

CAG

Available since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Rtn2b-mNeon

Plasmid

#186598

Purpose

Encodes human full-length Reticulon 2 isoform B fused to mNeonGreen

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Human Reticulon 2 isoform B (RTN2 Human)

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
NpY-mNeon

Plasmid

#186618

Purpose

Encodes Neuropeptide Y fused to mNeonGreen

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Neuropeptide Y (NPY Human)

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
mNeonGreen-Sec31A

Plasmid

#226582

Purpose

Encodes Sec31A protein labeled with mNeonGreen

Depositor

Gia Voeltz

Article

Westrate et al J Cell Sci. 2020 Jul 24;133(14). pii: jc

Insert

Sec31A (Protein transport protein) (SEC31A Human)

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-dfROX

Plasmid

#137170

Purpose

Lentiviral transfer vector for expression of roGFP2 and HyPer Red

Depositor

Shaun McCullough

Article

Faber et al Toxicol Sci. 2020 Jun 11. pii: 5856111.

Insert

roGFP2-2A-HyPer Red

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

April 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pF(UG) hSyn SYP-HaloTag

Plasmid

#188907

Purpose

Expresses synaptic HaloTag

Depositor

Edwin Chapman

Article

Vevea et al J Neurosci. 2023 May 10;43(19):3421-3438

Insert

SYP (Syp Mouse)

Use

Lentiviral

Tags

HaloTag

Expression

Mutation

Promoter

Available since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

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