We narrowed to 21,779 results for: Sis

1. pDSAP

   Plasmid

   #62293

   Purpose

   docking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, Puromycin resistance marker

   Depositor

   Eric Marois

   Article

   Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBHDuet50F

   Plasmid

   #118400

   Purpose

   analysis of cis-splicing by mini inteins

   Depositor

   Hideo Iwai

   Article

   Iwai et al J Mol Biol. 2017 Dec 8;429(24):3942-3956

   Insert

   MvuTFIIB mini intein
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBHDuet64

   Plasmid

   #118401

   Purpose

   analysis of cis-splicing by mini inteins

   Depositor

   Hideo Iwai

   Article

   Iwai et al J Mol Biol. 2017 Dec 8;429(24):3942-3956

   Insert

   MvuTFIIB mini intein
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSADuet779

   Plasmid

   #118395

   Purpose

   analysis of cis-splicing by mini inteins

   Depositor

   Hideo Iwai

   Article

   Iwai et al J Mol Biol. 2017 Dec 8;429(24):3942-3956

   Insert

   MjaTFIIB mini intein
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBHDuet61

   Plasmid

   #118396

   Purpose

   analysis of cis-splicing by mini inteins

   Depositor

   Hideo Iwai

   Article

   Iwai et al J Mol Biol. 2017 Dec 8;429(24):3942-3956

   Insert

   MjaTFIIB mini intein
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBHDuet45

   Plasmid

   #118393

   Purpose

   analysis of cis-splicing by mini inteins

   Depositor

   Hideo Iwai

   Article

   Iwai et al J Mol Biol. 2017 Dec 8;429(24):3942-3956

   Insert

   MjaTFIIB mini intein
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBHDuet173

   Plasmid

   #118398

   Purpose

   analysis of cis-splicing by mini inteins

   Depositor

   Hideo Iwai

   Article

   Iwai et al J Mol Biol. 2017 Dec 8;429(24):3942-3956

   Insert

   MvuTFIIB mini intein
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBSDONR P1-P4

   Plasmid

   #71744

   Purpose

   ampicillin resistant derivative of pDONR221 P1-P4 for multisite GATEWAY cloning

   Depositor

   Roger Innes

   Article

   Gu et al Plant Physiol. 2011 Apr;155(4):1827-38.

   Type

   Empty backbone

   Use

   Gateway entry vector for use in multisite gateway…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28a-At-TRL

   Plasmid

   #32242

   Depositor

   Stan Fields, Jay Hesselberth

   Article

   Schutz et al RNA. 2010 Mar;16(3):621-31. Epub 2010 Ja

   Insert

   A. thaliana tRNA ligase (RNL Mustard Weed)
   

   Use

   Tags

   6xHistidine

   Expression

   Bacterial

   Mutation

   Promoter

   T7 RNA Polmyerase

   Available since

   July 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBUN276

    Plasmid

    #60673

    Purpose

    overexpression of M. tuberculosis ddl gene in Mycobacterium

    Depositor

    Raul Barletta

    Article

    Feng et al Antimicrob Agents Chemother. 2003 Jan;47

    Insert

    ddl
    

    Use

    E.coli-mycobacterium shuttle vector

    Tags

    6 codons of M. bovis BCG hsp60

    Expression

    Mutation

    Promoter

    BCG Hsp60

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pBHDuet50K

    Plasmid

    #118399

    Purpose

    analysis of cis-splicing by mini inteins

    Depositor

    Hideo Iwai

    Article

    Iwai et al J Mol Biol. 2017 Dec 8;429(24):3942-3956

    Insert

    MvuTFIIB mini intein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    incorporation of oligonucleotide during cloning

    Promoter

    Available since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCS2TAL3_DD_NvFP-7R_Ex2-LEFT

    Plasmid

    #90036

    Purpose

    TALEN-mediated mutagenesis of Exon 2 in NvFP-7R

    Depositor

    Matthew Gibson

    Article

    Ikmi et al Nat Commun. 2014 Nov 24;5:5486. doi: 10.

    Insert

    7R_Ex2-LEFT
    

    Use

    TALEN

    Tags

    3x Flag and Fok1-DD domain

    Expression

    Mutation

    Promoter

    CMV

    Available since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCS2TAL3_RR_NvFP-7R_Ex2-RIGHT

    Plasmid

    #90037

    Purpose

    TALEN-mediated mutagenesis of Exon 2 in NvFP-7R

    Depositor

    Matthew Gibson

    Article

    Ikmi et al Nat Commun. 2014 Nov 24;5:5486. doi: 10.

    Insert

    7R_Ex2-RIGHT
    

    Use

    TALEN

    Tags

    Fok1-RR domain and HA

    Expression

    Mutation

    Promoter

    CMV

    Available since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pETSXM2-pLacI-mtrCAB

    Plasmid

    #174618

    Purpose

    Bacterial expression of gene cluster mtrCAB from Shewanella oneidensis MR-1.

    Depositor

    I-Son Ng

    Article

    I-Son Ng et al Bioresour. Bioprocess. 5, 35 (2018)

    Insert

    mtrCAB
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLacI

    Available since

    Nov. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMDC32-SEIPIN1

    Plasmid

    #96973

    Purpose

    Express Arabidopsis SEIPIN1 gene in plants.

    Depositor

    Kent Chapman

    Article

    Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

    Insert

    SEIPIN1 (AT5G16460 Mustard Weed)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    Aug. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pETSXM2-pLacI-mtrC

    Plasmid

    #174615

    Purpose

    Bacterial expression of mtrC from Shewanella oneidensis MR-1.

    Depositor

    I-Son Ng

    Article

    I-Son Ng et al Bioresour. Bioprocess. 5, 35 (2018)

    Insert

    MtrC
    

    Use

    Synthetic Biology; Shuttle vector in e. coli and …

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLacI

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. AL12R-EF-Tu

    Plasmid

    #161013

    Purpose

    PP7-tagged reporter driven by the Arabidopsis EF-Tu promoter

    Depositor

    Krishna Niyogi

    Article

    Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

    Insert

    PP7 and H2B-mScarlet
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Dec. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAtLCYbSK

    Plasmid

    #53588

    Purpose

    Produces an active Arabidopsis thaliana lycopene beta-cyclase enzyme in E. coli. Also known as pAt9SK.

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Eukaryot Cell. 2007 Mar;6(3):533-45. Epu

    Insert

    lcyB (LYC Mustard Weed)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMDC32-SEIPIN3

    Plasmid

    #96975

    Purpose

    Express Arabidopsis SEIPIN3 gene in plants.

    Depositor

    Kent Chapman

    Article

    Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

    Insert

    SEIPIN3 (AT2G34380 Mustard Weed)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    Aug. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGG-PIP72

    Plasmid

    #196810

    Purpose

    Contains PMR4 promoter

    Depositor

    Marc Somssich

    Article

    Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

    Insert

    POWDERY MILDEW RESISTANT 4
    

    Use

    Greengate (gg) entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. mCherry-C1-FKBPflex-OSBP-siRNAr

    Plasmid

    #220066

    Purpose

    siRNA resistant ORP5 attcahed with anFKBP and a mCherry fluorophore

    Depositor

    Gerry Hammond

    Article

    Doyle et al Contact (Thousand Oaks). 2024 Feb 22;7:2

    Insert

    OSBPL5 siRNA resistant (OSBPL5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    siRNA resistant

    Promoter

    Available since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLBC0120

    Plasmid

    #172791

    Purpose

    Level 0 module containing CDS2ns_mHSP21

    Depositor

    Benjamin Field

    Article

    Velay et al Plant Methods. 2022 May 26;18(1):69. doi

    Insert

    CDS mature HSP21 (44-227), Arabidopsis thaliana
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    no

    Promoter

    Available since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLBC0130

    Plasmid

    #172792

    Purpose

    Level 0 module containing CDS2ns_mPTAC5

    Depositor

    Benjamin Field

    Article

    Velay et al Plant Methods. 2022 May 26;18(1):69. doi

    Insert

    CDS mature PTAC5 (41-387), Arabidopsis thaliana
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    no

    Promoter

    Available since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pYC1640

    Plasmid

    #158719

    Purpose

    CRISPR system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses Sth1 Cas9 and the cognate sgRNA, using zeocin as a selection marker.

    Depositor

    Yi-Cheng Sun

    Article

    Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

    Insert

    Sth1 sgRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pICH47742:: pRPS5a-PE2max-NC::tHSP

    Plasmid

    #227694

    Purpose

    Golden Gate level 1, position 2, PE2max-NC expression cassette for Arabidopsis.

    Depositor

    Jae-Yean Kim

    Article

    Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

    Insert

    PE2max-NC being driven by pRPS5a and terminated by tHSP
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    pRPS5a

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. (+)-Limonene Synthase

    Plasmid

    #112230

    Purpose

    (+)-Limonene Synthase from Citrus sinensis in pET-28a(+). N-terminal was truncated to remove amino acids 1-52 and then His-tagged for purification.

    Depositor

    Daniel Oprian

    Article

    Morehouse et al Biochemistry. 2017 Mar 28;56(12):1706-17

    Insert

    (+)-Limonene Synthase
    

    Use

    Tags

    N-terminal His-tag

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-52

    Promoter

    Available since

    June 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. 35S::GeNL/pCAMBIA1301

    Plasmid

    #190070

    Purpose

    Expressing bioluminescent pH indicator-GeNL- in Arabidopsis thaliana

    Depositor

    Takeharu Nagai

    Article

    Tran et al Plant Cell Environ. 2022 Jul 21. doi: 10

    Insert

    Green-enhanced Nano-lantern
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S

    Available since

    April 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. AL12R-HSP101

    Plasmid

    #161014

    Purpose

    PP7-tagged reporter driven by the Arabidopsis HSP101 promoter

    Depositor

    Krishna Niyogi

    Article

    Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

    Insert

    PP7 and H2B-mScarlet
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCP-tRNA

    Plasmid

    #133812

    Purpose

    CRISPR-Cas9 system for genetic manipulation of Candida parapsilosis, C. orthopsilosis, and C. metapsilosis

    Depositor

    Geraldine Butler

    Article

    Lombardi et al mSphere. 2019 Mar 13;4(2). pii: 4/2/e001

    Insert

    cassette for the expression of the sgRNA from the C. parapsilosis RNA pol II GAPDH promoter
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pYC2085

    Plasmid

    #158721

    Purpose

    CRISPR system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses Sth1 Cas9 and the cognate sgRNA, using hygromycin as a selection marker.

    Depositor

    Yi-Cheng Sun

    Article

    Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364

    Insert

    Sth1 sgRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pAC-4CL1

    Plasmid

    #35947

    Depositor

    Claudia Schmidt-dannert

    Article

    Watts et al BMC Biotechnol. 2006 Mar 21;6:22.

    Insert

    4-coumaroyl CoA ligase 1 (4CL1 Mustard Weed)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLac-mod

    Available since

    Sept. 5, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pY2F

    Plasmid

    #53285

    Purpose

    Contains an Arabidopsis thaliana lcyE cDNA and produces an active lycopene epsilon-cyclase enzyme in E. coli. Use with pAC-BETA-At or pAC-BETAipi-At to produce alpha-carotene in E. coli.

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

    Insert

    lcyE (LUT2 Mustard Weed)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    April 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. AL12R-HSP70

    Plasmid

    #161010

    Purpose

    PP7-tagged reporter driven by the Arabidopsis HSP70 promoter

    Depositor

    Krishna Niyogi

    Article

    Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

    Insert

    PP7 and H2B-mScarlet
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Dec. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pETCON-PTX-S1-220

    Plasmid

    #67747

    Purpose

    Pertussis Toxin Subunit 1 Yeast Display Plasmid

    Depositor

    Timothy Whitehead

    Article

    Kowalsky et al J Biol Chem. 2015 Oct 30;290(44):26457-7

    Insert

    PTxS1-220
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAtCHYb1Δ129N

    Plasmid

    #53367

    Purpose

    Contains a truncated Arabidopsis beta-carotene hydroxylase cDNA, the product of which converts beta-carotene primarily to monocyclic beta-cryptoxanthin in E. coli. Use with pAC-BETA or pAC-BETAipi.

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al Plant J. 2005 Feb;41(3):478-92.

    Insert

    chyB1 (BETA-OHASE 1 Mustard Weed)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Deleted codons for the first 129 amino acids

    Promoter

    Trc

    Available since

    April 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. AL13Rb-GAPC2

    Plasmid

    #161009

    Purpose

    PP7-tagged reporter driven by the Arabidopsis GAPC2 promoter

    Depositor

    Krishna Niyogi

    Article

    Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

    Insert

    PP7 and H2B-mScarlet
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Dec. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAtipiTrc

    Plasmid

    #53275

    Purpose

    Contains an Arabidopsis thaliana idi1 cDNA fused to a strong Trc promoter. Produces an active isopentenyl diphosphate isomerase (IDI) in E. coli.

    Depositor

    Francis X Cunningham Jr

    Article

    Cunningham FX et al J Bacteriol. 2000 Oct;182(20):5841-8.

    Insert

    ipi1 (IPP2 Mustard Weed)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Trc

    Available since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET21a - Mtb-TF

    Plasmid

    #111513

    Purpose

    Expresses M. tuberculosis Trigger Factor with a C-terminal His tag

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Cell. 2017 Nov 16;171(5):1125-1137.e11.

    Insert

    Trigger Factor
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pRS315-PGK-SEIPIN1

    Plasmid

    #96986

    Purpose

    Express GFP-tagged Arabidopsis SEIPIN1 gene in yeast cells.

    Depositor

    Kent Chapman

    Article

    Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

    Insert

    SEIPIN1 (AT5G16460 Mustard Weed)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Phosphoglycerate Kinase (PGK) promoter

    Available since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pLBC0010

    Plasmid

    #172774

    Purpose

    Level 0 module containing NT1_CTPSSU

    Depositor

    Benjamin Field

    Article

    Velay et al Plant Methods. 2022 May 26;18(1):69. doi

    Insert

    Gene sequence for Rubisco small subunit (SSU) chloroplast transit peptide (CTP) of Arabidopsis thaliana
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    no

    Promoter

    Available since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge