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Plasmid#201444PurposeLow copy vector for expressing human DNA polymerase iota in E. coli with an N-terminal 6x His tagDepositorInsertDNA polymerase iota (POLI Human)
UseTags6x HisExpressionBacterialMutationCoding sequence has been optimised for expression…PromoterlacIqAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
ALIX(P801G)-mNeonGreen
Plasmid#191192PurposeFull-length ALIX with P801G mutation in proline-rich domain; C-terminally tagged with mNeonGreen.DepositorInsertALIX (P801G) (PDCD6IP Human, Synthetic)
UseTags6xHIS and mNeonGreenExpressionMammalianMutationPro 801 mutated to GlyPromoterCMVAvailable sinceOct. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
MAC_C_CSF1R
Plasmid#187767PurposeMAC-tagged gene expression of human CSF1RDepositorInsertCSF1R (CSF1R Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
MAC_C_ERBB3
Plasmid#187794PurposeMAC-tagged gene expression of human ERBB3DepositorInsertERBB3 (ERBB3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHA852 - mec-4p::FynY531F
Plasmid#139209PurposeExpresses activated Fyn kinase in mec-4 neuronsDepositorInsertFyn (FYN Human)
UseTagsExpressionWormMutationY531FPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
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hnRNPA2_LC_RtoK
Plasmid#139120Purposeexpress hnRNPA2 LC where all R are KDepositorInserthnRNPA2 (HNRNPA2B1 Human)
UseTagsExpressionBacterialMutationall R to KPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
hnRNPA2_LC_NtoS
Plasmid#139121Purposeexpress hnRNPA2 with all N changed to SDepositorInserthnRNPA2 (HNRNPA2B1 Human)
UseTagsExpressionBacterialMutationall N to SPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
hnRNPA2_LC_5E
Plasmid#139122Purposeexpress hnRNPA2 with 5 serine changed to glutamateDepositorInserthnRNPA2 (HNRNPA2B1 Human)
UseTagsExpressionBacterialMutation5 serine to glutamatePromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
hnRNPA2_LC_12E
Plasmid#139123Purposeexpress hnRNPA2 with all serine change to glutamateDepositorInserthnRNPA2 (HNRNPA2B1 Human)
UseTagsExpressionBacterialMutation12 S to EPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
hnRNPF_PLD_YtoS
Plasmid#139124Purposeexpress hnRNPF PLD with all Y changed to SDepositorInserthnRNPA2 (HNRNPA2B1 Human)
UseTagsExpressionBacterialMutationall Y to SPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
hnRNPF_PLD_StoA
Plasmid#139125Purposeexpress hnRNPF PLD with all S changed to ADepositorInserthnRNPA2 (HNRNPA2B1 Human)
UseTagsExpressionBacterialMutationall S to APromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET151-GST-PP-RPN13 (19-132) K97E
Plasmid#125825PurposeExpresses GST-RPN13 (19-132) K97E in E.coliDepositorInsertRPN13 (ADRM1 Human)
UseTagsGSTExpressionBacterialMutationchanged lysine 97 to glutamatePromoterT7Available sinceAug. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET151-GST-PP-RPN13 (19-132) K83A
Plasmid#125829PurposeExpresses GST-RPN13 (19-132) K83A in E.coliDepositorInsertRPN13 (ADRM1 Human)
UseTagsGSTExpressionBacterialMutationchanged lysine 83 to alaninePromoterT7Available sinceMay 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET151-GST-PP-RPN13 (19-132) K97A
Plasmid#125823PurposeExpresses GST-RPN13 (19-132) K97A in E.coliDepositorInsertRPN13 (ADRM1 Human)
UseTagsGSTExpressionBacterialMutationchanged lysine 97 to alaninePromoterAvailable sinceMay 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET151-GST-PP-RPN13 (19-132) K97D
Plasmid#125824PurposeExpresses GST-RPN13 (19-132) K97D in E.coliDepositorInsertRPN13 (ADRM1 Human)
UseTagsGSTExpressionBacterialMutationchanged lysine 97 to aspartatePromoterT7Available sinceMay 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET22b-ecDHFR-D27S/Y100F
Plasmid#110827PurposeBacterial expression of E.coli DHFR D27S/Y100F mutantDepositorInsertecDHFR
UseTagsExpressionBacterialMutationD27 mutated to S27, Y100 mutated to F100PromoterT7Available sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDEF3‐hTIM3 (no tag)
Plasmid#49212Purposeexpresses human Tim3DepositorInsertTim3 (HAVCR2 Human)
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceDec. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMX-SHP1C/S-ires-mCD8
Plasmid#183719PurposeExpresses the myc-tagged SHP-1 C453S mutant (SHP-1C/S) (substrate-trapping mutant) and mouse CD8.DepositorInsertSHP-1
UseRetroviralTagsmycExpressionMutationC453SPromoterAvailable sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX Zap70(NC)-SH2
Plasmid#46535DepositorInsertZap70(NC) (ZAP70 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 1-268PromoterTacAvailable sinceAug. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-TRKB-V5/HIS
Plasmid#205614PurposeExpression of human TRKB receptor tyrosine kinase in mammalian cellsDepositorInsertTRKB receptor tyrosine kinase (NTRK2 Human)
UseTagsV5-HisExpressionMammalianMutationPromoterCMVAvailable sinceSept. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-RET-V5/HIS
Plasmid#205606PurposeExpression of human RET receptor tyrosine kinase in mammalian cellsDepositorInsertRET receptor tyrosine kinase (RET Human)
UseTagsV5-HisExpressionMammalianMutationPromoterCMVAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-TEK-V5/HIS
Plasmid#205611PurposeExpression of human TEK receptor tyrosine kinase in mammalian cellsDepositorInsertTEK receptor tyrosine kinase (TEK Human)
UseTagsV5-HisExpressionMammalianMutationPromoterCMVAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-HA_WT RAD23A
Plasmid#201458PurposeExpresses human RAD23A with an N-terminal HA tag in mammalian cellsDepositorInsertUV excision repair protein RAD23 homolog A (RAD23A Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
PTPRJ-bio-His
Plasmid#51816PurposeExpresses full-length Receptor-type tyrosine-protein phosphatase eta precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertPTPRJ (PTPRJ Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGLO-GFP-1UAG
Plasmid#82500PurposeExpresses GFP with 1 UAG codon at the amino acid position 3DepositorInsertGreen Fluorescent Protein with 1 UAG codon
UseTagsExpressionBacterialMutationAdded an UAG codon at the amino acid position 3PromoterAraBADAvailable sinceSept. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pIRES2-EGFP-SHP2WT
Plasmid#12283DepositorInsertSHP-2WT (PTPN11 Human)
UseTagsMyc (not canonical: KLISEEDL)ExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-PDGFRB-V5/HIS
Plasmid#201988Purposeexpression of human PDGFRB receptor tyrosine kinase in mammalian cellsDepositorInsertPDGFRB receptor tyrosine kinase (PDGFRB Human)
UseTagsV5, 6xHisExpressionMammalianMutationPromoterCMVAvailable sinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-TRKC-V5/HIS
Plasmid#205615PurposeExpression of human TRKC receptor tyrosine kinase in mammalian cellsDepositorInsertTRKC receptor tyrosine kinase (NTRK3 Human)
UseTagsV5-HisExpressionMammalianMutationPromoterCMVAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCB’ CbbL Y72R
Plasmid#162707PurposeCarboxysome operon with point mutation to CbbL, prk; Substitution of CsoS2-binding tyrosine with arginine prevents rubisco encapsulation creating rubisco-less carboxysomesDepositorInsertpHnCB10 derived carboxysome operon, prk
UseTagsExpressionBacterialMutationCbbL Y72R; Substitution of CsoS2-binding tyrosine…PromoterPLtet0-1 promoterAvailable sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-RON-V5/HIS
Plasmid#201983Purposeexpression of human RON receptor tyrosine kinase in mammalian cellsDepositorInsertRON receptor tyrosine kinase (MST1R Human)
UseTagsV5, 6xHisExpressionMammalianMutationPromoterCMVAvailable sinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLINK cyclin T1 (1-280) myc (P#586)
Plasmid#14612DepositorInsertcyclin T1 (1-280) (CCNT1 Human)
UseTagsmycExpressionMammalianMutationPromoterAvailable sinceJune 30, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAAV-ITR-EcYtR-mCherry
Plasmid#217362PurposeExpresses E. coli tyrosine tRNA and a wild-type mCherry reporter; can be packaged into AAVDepositorInsertE. coli tyrosine tRNA
UseAAVTagsExpressionMammalianMutationPromoterU6Available sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-RYK-V5/HIS
Plasmid#205610PurposeExpression of human RYK receptor tyrosine kinase in mammalian cellsDepositorInsertRYK receptor tyrosine kinase (RYK Human)
UseTagsV5-HisExpressionMammalianMutationPromoterCMVAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR Grb2-TagBFP
Plasmid#188631PurposeHuman Grb2 C-terminally labeled with TagBFPDepositorInsertGRB2 (GRB2 Human, Synthetic)
UseLentiviralTagsTagBFPExpressionMammalianMutationPromoterSFFVAvailable sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
SpRYc
Plasmid#175575PurposeExpresses the codon-optimized, engineered SpRYc Cas9 nuclease in mammalian cellsDepositorInsertSpRYc
UseTagsEGFPExpressionMammalianMutationPID of SpRY grafted onto the N-terminal domain of…PromoterCMVAvailable sinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIRES2-EGFP-SHP2CS
Plasmid#12284DepositorInsertSHP-2CS (PTPN11 Human)
UseTagsMycExpressionMammalianMutationCysteine 459 mutated to SerinePromoterAvailable sinceAug. 11, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBABE-puro SHP2 E76A
Plasmid#8331DepositorInsertSHP2 (PTPN11 Human)
UseRetroviralTagsExpressionMammalianMutationE76A (activated mutant)PromoterAvailable sinceJune 17, 2005AvailabilityAcademic Institutions and Nonprofits only -
hnRNPA2_LC_nocharge
Plasmid#139118Purposeexpress hnRNPA2 LC with no charged residuesDepositorInserthnRNPA2 (HNRNPA2B1 Human)
UseTagsExpressionBacterialMutationall charged residues are changed to S/Q except fo…PromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX CrkL-SH2
Plasmid#46419DepositorInsertCrkl (CRKL Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 9-108PromoterTacAvailable sinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only