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pMSCV-FKBP-TRIF(trunc 1-83)

Plasmid

#221250

Purpose

Expression of TRIF truncation 4 (84-222) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

TRIF truncation 4 (84-222) (Ticam1 Mouse)

Use

Retroviral

Tags

3xFLAG-4xFKBP

Expression

Mutation

Promoter

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-TRIF(trunc 1-23)

Plasmid

#221247

Purpose

Expression of TRIF truncation 1 (24-222) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

TRIF truncation 1 (24-222) (Ticam1 Mouse)

Use

Retroviral

Tags

3xFLAG-4xFKBP

Expression

Mutation

Promoter

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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Hygro-P2A-HA-FKBP-WAPL

Plasmid

#217666

Purpose

Repair template to endogenously tag WAPL with Hygro-P2A-HA-FKBP

Depositor

Daniel Gerlich

Article

Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

Insert

WapI (WAPL Human)

Use

Tags

dTag (FKBP36V protein)

Expression

Bacterial and Mammalian

Mutation

Promoter

Lac

Available since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-FKBP-GFP-OPTN

Plasmid

#208866

Purpose

Stably express GFP-tagged OPTN for CID induced mitophagy

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv September 25, 202

Insert

OPTN (OPTN Human)

Use

Tags

FKBP-GFP

Expression

Mammalian

Mutation

Promoter

Available since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-C1-FKBPflex-ELL-CAAX

Plasmid

#220076

Purpose

an extra long linker attached to a CAAX domain with an mCherry tag

Depositor

Gerry Hammond

Article

Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

Insert

CAAX (HRAS Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-C1-FKBPflex-OSBP-siRNAr

Plasmid

#220066

Purpose

siRNA resistant ORP5 attcahed with anFKBP and a mCherry fluorophore

Depositor

Gerry Hammond

Article

Doyle et al Contact (Thousand Oaks). 2024 Feb 22;7:2

Insert

OSBPL5 siRNA resistant (OSBPL5 Human)

Use

Tags

Expression

Mammalian

Mutation

siRNA resistant

Promoter

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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FKBP12-CB2mutx2-mTagBFP2-HAx2

Plasmid

#207147

Purpose

Tandem mutant calcium-buffer with rapalog dimerizer

Depositor

Antony Galione

Article

Davis et al EMBO J. 2020 Jul 15;39(14):e104058. doi:

Insert

Calbindin-2 (CALB2 Human)

Use

Tags

FKBP12, HA, and mTagBFP2

Expression

Mammalian

Mutation

E40Q, E87Q, E131Q, E175Q, E219Q

Promoter

CMV

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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FKBP12-talinC11-ABS3mut-mEmerald

Plasmid

#202383

Purpose

FKBP12-Talin(1974-2541)(K2443D)(V2444D)(K2445D)-mEmerald

Depositor

Pakorn Kanchanawong

Article

Wang et al Nano Lett. 2019 Oct 9;19(10):7514-7525.

Insert

FKBP12-Talin(1974-2541)(K2443D)(V2444D)(K2445D)-mEmerald

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-PIP4K2A-Kina

Plasmid

#202745

Purpose

expresses recruitable fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

mCherry:FKBP1A(3-108) : [GGSA]4GG :PIP4K2A (33-406) (PIP4K2A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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pBetaActin-KIF1aMotor-mScarlet-FKBP

Plasmid

#191336

Purpose

Expresses the fluorescently labeled plus-end directed KIF1a motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

KIF1aMotor-mScarlet-FKBP (Kif1a Mouse)

Use

Tags

Expression

Mammalian

Mutation

motor domain aa 1-405

Promoter

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pBetaActin-FKBP-Halo-Dync1h1motor

Plasmid

#191333

Purpose

Expresses the fluorescently labeled dynein motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

FKBP-Halo-Dync1h1motor (Dync1h1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

DYNC1H1 motor domain aa 1453-4644

Promoter

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pBetaActin-FKBP-mScarlet-KIFC1motorN593K

Plasmid

#191335

Purpose

Expresses the fluorescently labeled minus-end directed KIFC1 motor-deficient motor domain fused to FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

FKBP-mScarlet-KIFC1N593K (KIFC1 Human)

Use

Tags

Expression

Mammalian

Mutation

motor domain aa 125-673; N593K

Promoter

Available since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pBetaActin-FKBP-mScarlet-Dync1h1motorK2599T

Plasmid

#191331

Purpose

Expresses the fluorescently labeled motor-deficient dynein motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

FKBP-mScarlet-Dync1h1motorK2599T (Dync1h1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

DYNC1H1 motor domain aa 1453-4644

Promoter

Available since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pfa6a mScarlet-FKBP::KANMX

Plasmid

#185905

Purpose

Plasmid for cloning mScarlet fused to FKBP into yeast.

Depositor

Matthew Good

Article

Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

Insert

mScarlet-FKBP

Use

Tags

mScarlet fused to FKBP

Expression

Yeast

Mutation

Promoter

SP6

Available since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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mCh-2xFKBP (no insert) (pBS1139)

Plasmid

#185322

Purpose

The empty vector used for the FKBP experiments in our paper

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only
CAHS1_PARRC_101-189_mCh-2xFKBP (pBS1136)

Plasmid

#185320

Purpose

For the mammalian expression of the tardigrade protein CAHS1_PARRC_101-189 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

CAHS1_PARRC_101-189

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
CAHS2_PARRC_98-130_mCh-2xFKBP (pBS1120)

Plasmid

#185315

Purpose

For the mammalian expression of the tardigrade protein CAHS2_PARRC_98-130 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

CAHS2_PARRC_98-130

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
DSUP_RAMVA_1-208_mCh-2xFKBP (pBS1116)

Plasmid

#185311

Purpose

For the mammalian expression of the tardigrade protein DSUP_RAMVA_1-208 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

DSUP_RAMVA_1-208

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
ApoE3_D125I_47-300_mCh-2xFKBP (pBS1114)

Plasmid

#185309

Purpose

For the mammalian expression of the human protein ApoE3_D125I_47-300 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

ApoE3_D125I_47-300

Use

Tags

Expression

Mammalian

Mutation

D125I

Promoter

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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ApoE3_47-300_mCh-2xFKBP (pBS1113)

Plasmid

#185308

Purpose

For the mammalian expression of the human protein ApoE3_47-300 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

ApoE3_47-300

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge