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pcDNA3-RIPK3[S199D][S227D]-FLAG

Plasmid

#199324

Purpose

double mutated phosphorylation site (kinase acitivity and MLKL interaction active)

Depositor

Adrian Press

Publication

Hoff et al Cell Death Dis. 2023 Apr 18;14(4):275. d

Insert

Receptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed Serine 199 and 227 to Aspartic Acid

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pSPL3-ABCC8-9-10

Plasmid

#135918

Purpose

Encodes ABCC8 exons 9 and 10 wild type for the analysis of splicing variants

Depositor

Diana Valverde Pérez

Publication

Lago-Docampo et al Sci Rep. 2020 Sep 15;10(1):15135. doi: 1

Insert

ATP-binding Cassette subfamily C member 8 (ABCC8 Human)

Use

Exon trapping

Expression

Mammalian

Promoter

SV40

Available since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pSPL3-ABCC8-27

Plasmid

#135919

Purpose

Encodes ABCC8 exon 27 wild type for the analysis of splicing variants

Depositor

Diana Valverde Pérez

Publication

Lago-Docampo et al Sci Rep. 2020 Sep 15;10(1):15135. doi: 1

Insert

ATP-binding Cassette subfamily C member 8 (ABCC8 Human)

Use

Exon trapping

Expression

Mammalian

Promoter

SV40

Available since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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R713-M52-303: CMV51p> Halotag-NRasHVR

Plasmid

#159686

Purpose

Mammalian protein expression of NRAS HVR (Hypervariable region)-Halotag fusion

Depositor

Dominic Esposito

Publication

Goswami et al Elife. 2020 Jan 20;9. pii: 47654. doi: 1

Insert

Halotag-NRasHVR (NRAS Human)

Tags

Halotag

Expression

Mammalian

Available since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pCytERM-hfYFP

Plasmid

#186520

Purpose

Visualization of the ER in cells using hyperfolder YFP (compatible with OSER assay)

Depositor

Gregory Petsko

Publication

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

Hyperfolder YFP

Tags

Cytochrome p450 first 29 animo acids (Oryctolagus…

Expression

Mammalian

Mutation

mGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…

Promoter

CMV

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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R713-M50-303: CMV51p> Halotag-HRasHVR

Plasmid

#159685

Purpose

Mammalian protein expression of HRAS HVR (Hypervariable region)-Halotag fusion

Depositor

Dominic Esposito

Publication

Goswami et al Elife. 2020 Jan 20;9. pii: 47654. doi: 1

Insert

Halotag-HRasHVR (HRAS Human)

Tags

Halotag

Expression

Mammalian

Available since

Sept. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pRC/RSV-rat Agt

Plasmid

#122104

Purpose

Expression of angiotensinogen

Depositor

John Chan

Publication

Zhang et al J Am Soc Nephrol. 2002 Feb;13(2):302-12.

Insert

angiotensinogen (Agt Rat)

Expression

Mammalian

Promoter

RSV promoter

Available since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Flag-PGC-1alpha2

Plasmid

#45502

Depositor

Jorge Ruas, Bruce Spiegelman

Publication

Ruas et al Cell. 2012 Dec 7;151(6):1319-31. doi: 10

Insert

PGC-1alpha Isoform2 (Ppargc1a Mouse)

Tags

6xHis and Flag

Expression

Mammalian

Promoter

CMV

Available since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-ITR-PytR(PyOtR)-mCherry

Plasmid

#204870

Purpose

Expresses pyrrolysyl tRNA variant "PyOtR" and a wild-type mCherry reporter; can be packaged into AAV

Depositor

Abhishek Chatterjee

Publication

Jewel et al Angew Chem Int Ed Engl. 2024 Jan 26:e202

Insert

M. mazei pyrrolysyl tRNA mutant "PyOtR"

Use

AAV

Expression

Mammalian

Mutation

U25C; A3G, A4C, A5G, C6G, G63C, T65G, T66C

Promoter

U6

Available since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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EGFP-plk1-AKAP

Plasmid

#132967

Purpose

To study the processes involved in early mitotic events, mitotic progression, and control of re-entry after DNA damage

Depositor

Michael Yaffe

Publication

Kishi et al Mol Cell Biol. 2009 Jun;29(11):3134-50.

Insert

polo-like kinase 1-AKAP (PLK1, AKAP9 Human)

Expression

Mammalian

Mutation

C terminus of full-length Plk1 fused to the peric…

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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FLAG-RFP-UCR1C

Plasmid

#67582

Purpose

Expresses C-terminal amino acids of UCR1 region of PDE4D3 in mammalian cells

Depositor

Graeme Carnegie

Publication

Wang et al Cell Signal. 2015 May;27(5):908-22. doi:

Insert

C-terminus of upstream conserved region 1 of phosphodiesterase 4D3 (PDE4D Human)

Tags

FLAG and TagRFP

Expression

Mammalian

Mutation

Amino acid residues 80-136 of PDE4D3

Available since

Oct. 14, 2015

Availability

Academic Institutions and Nonprofits only
R713-M48-303: CMV51p> Halotag-Kras4aHVR

Plasmid

#159684

Purpose

Mammalian protein expression of KRAS4a HVR (Hypervariable region)-Halotag fusion

Depositor

Dominic Esposito

Publication

Goswami et al Elife. 2020 Jan 20;9. pii: 47654. doi: 1

Insert

Halotag-Kras4aHVR (KRAS Human)

Tags

Halotag

Expression

Mammalian

Available since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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R703-M80-303: CMV51p> Halotag-Kras4bHVR

Plasmid

#159683

Purpose

Mammalian protein expression of KRAS4b HVR (Hypervariable region)-Halotag fusion

Depositor

Dominic Esposito

Publication

Goswami et al Elife. 2020 Jan 20;9. pii: 47654. doi: 1

Insert

Halotag-Kras4bHVR (KRAS Human)

Tags

Halotag

Expression

Mammalian

Available since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pGRNA3_LacZ_IB1_IIT1

Plasmid

#247080

Purpose

L-arabinose inducible crRNA of type I-E CRISPR cas (Escherichia coli K-12). encoding 105 nt spacer which targets both Ecoli LacZ B1 and T1 site. riboJ/RNaseIII site are encoded between two crRNA

Depositor

Seokhee Kim

Publication

Lee et al Nat Commun. 2025 Nov 25. doi: 10.1038/s4

Insert

type I-E CRISPR cas crRNA

Use

CRISPR

Expression

Bacterial

Available since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pGRNA3_LacZ_B1_IIB5

Plasmid

#247081

Purpose

L-arabinose inducible crRNA of type I-E CRISPR cas (Escherichia coli K-12). encoding 105 nt spacer which targets both Ecoli LacZ B1 and B5 site

Depositor

Seokhee Kim

Publication

Lee et al Nat Commun. 2025 Nov 25. doi: 10.1038/s4

Insert

type I-E CRISPR cas crRNA

Use

CRISPR

Expression

Bacterial

Available since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pVAM07

Plasmid

#252057

Purpose

Hygromycin B resistant derivative of pKEK2201 for allelic exchange

Depositor

James Bina

Publication

Van Allen et al J Microbiol Methods. 2025 Jan;228:107070

Type

Empty backbone

Use

Sucrose counter-selection allelic exchange vector

Available since

March 13, 2026

Availability

Academic Institutions and Nonprofits only

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pGRNA1_105nt

Plasmid

#247075

Purpose

L-arabinose inducible crRNA of type I-E CRISPR cas (Escherichia coli K-12). encoding 105 nt spacer which targets pheS*_codon

Depositor

Seokhee Kim

Publication

Lee et al Nat Commun. 2025 Nov 25. doi: 10.1038/s4

Insert

type I-E CRISPR cas crRNA

Use

CRISPR

Expression

Bacterial

Available since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pGRNA2II_LacZT1

Plasmid

#247079

Purpose

L-arabinose inducible crRNA of type I-E CRISPR cas (Escherichia coli K-12). encoding 105 nt spacer which targets Ecoli LacZ T1 site

Depositor

Seokhee Kim

Publication

Lee et al Nat Commun. 2025 Nov 25. doi: 10.1038/s4

Insert

type I-E CRISPR cas crRNA

Use

CRISPR

Expression

Bacterial

Available since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pMut19_tadDE16B

Plasmid

#247073

Purpose

L-arabinose inducible TadDE-XTEN16-CasB(Cas11)-GSSG-UGI

Depositor

Seokhee Kim

Publication

Lee et al Nat Commun. 2025 Nov 25. doi: 10.1038/s4

Insert

TadDE-XTEN16-CasB-UGI

Use

CRISPR

Expression

Bacterial

Available since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pYPQ133-RZ-Cas12j2

Plasmid

#189784

Purpose

Golden Gate entry vector to clone the 3rd Cas12j2(CasΦ) crRNA flanked by HH and HDV ribozymes

Depositor

Yiping Qi

Publication

Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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