We narrowed to 908 results for: pgem

1. pGEM-and3 in situ probe

   Plasmid

   #52104

   Purpose

   actinodin 3 and3 antisense in situ probe linearize w/ ApaI SP6 pol

   Depositor

   Didier Stainier

   Article

   Nolte et al Proteomics. 2015 Feb;15(4):739-51. doi:

   Insert

   actinodin 3 in situ probe (and3 Zebrafish)
   

   Expression

   Bacterial

   Available Since

   March 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEMT-Map7-1783-end

   Plasmid

   #46071

   Depositor

   Edgar Gomes

   Article

   Metzger et al Nature. 2012 Mar 18;484(7392):120-4. doi

   Insert

   Map7-1783-end (Map7 Mouse)
   

   Expression

   Bacterial

   Available Since

   July 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEMT-Map7-856-1351

   Plasmid

   #46069

   Depositor

   Edgar Gomes

   Article

   Metzger et al Nature. 2012 Mar 18;484(7392):120-4. doi

   Insert

   Map7-856-1351 (Map7 Mouse)
   

   Use

   Unspecified

   Expression

   Bacterial

   Available Since

   July 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. Torso cDNA Y918F in pGEM

   Plasmid

   #39797

   Depositor

   Norbert Perrimon

   Article

   Gayko et al Proc Natl Acad Sci U S A. 1999 Jan 19;96

   Insert

   Torso (tor Fly)
   

   Expression

   Bacterial

   Mutation

   Y918F

   Available Since

   Oct. 16, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pGEM4Z-T7-5'UTR-EGFP-3'UTR-A64

   Plasmid

   #203348

   Purpose

   Template for T7 in vitro transcription of EGFP mRNA flanked by Xenopus beta globin UTRs and 64nt polyA tail

   Depositor

   Molly Stevens, Christopher Grigsby

   Insert

   Enhanced Green Fluorescent Protein
   

   Use

   Template for t7-mediated in vitro transcription o…

   Tags

   Flanked by 5' and 3' UTRs from Xenopus …

   Promoter

   T7

   Available Since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEM4Z-T7-5'UTR-NanoLuc-3'UTR-A64

   Plasmid

   #203350

   Purpose

   Template for T7 in vitro transcription of NanoLuc mRNA flanked by Xenopus beta globin UTRs and 64nt polyA tail

   Depositor

   Molly Stevens, Christopher Grigsby

   Insert

   Nano Luciferase
   

   Use

   Template for t7-mediated in vitro transcription o…

   Tags

   Flanked by 5' and 3' UTRs from Xenopus …

   Promoter

   T7

   Available Since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEM4Z-T7-5'UTR-mGreenLantern-3'UTR-A64

   Plasmid

   #203349

   Purpose

   Template for T7 in vitro transcription of mGreenLantern mRNA flanked by Xenopus beta globin UTRs and 64nt polyA tail

   Depositor

   Molly Stevens, Christopher Grigsby

   Insert

   mGreenLantern
   

   Use

   Template for t7-mediated in vitro transcription o…

   Tags

   Flanked by 5' and 3' UTRs from Xenopus …

   Promoter

   T7

   Available Since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEMT-PT2A-GFP-Tdtomato-iRFP670

   Plasmid

   #111812

   Purpose

   Tricistronic construct: Co-express three fluorescent proteins by P2A and then T2A

   Depositor

   Li Qian

   Article

   Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

   Inserts

   GFP
   Tdtomato
   iRFP670
   

   Use

   Cloning intermediate

   Available Since

   Aug. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEM-NLS-Cre-2A-mCherry-FKF

   Plasmid

   #119868

   Purpose

   Donor cassette containing NLS-tagged Cre, a Thosea asigna virus peptide (2A), mCherry and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

   Depositor

   Tatjana Sauka-Spengler

   Article

   Weinberger et al Dev Cell. 2020 Mar 9;52(5):574-590.e6. d

   Insert

   NLS-Cre, 2A, mCherry and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
   

   Tags

   mCherry

   Expression

   Bacterial

   Available Since

   April 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEMT-PT2A-iRFP670-Tdtomato-GFP

    Plasmid

    #111817

    Purpose

    Tricistronic construct: Co-express three fluorescent proteins by P2A and then T2A

    Depositor

    Li Qian

    Article

    Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

    Inserts

    iRFP670
    Tdtomato
    GFP
    

    Use

    Cloning intermediate

    Available Since

    Dec. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEM-human TCR zeta/2470

    Plasmid

    #11507

    Depositor

    Allan Weissman

    Article

    Weissman et al Proc Natl Acad Sci U S A. 1988 Dec . 85(

    Insert

    TCR zeta (CD247 Human)
    

    Use

    General cloning

    Mutation

    n/a

    Available Since

    March 8, 2006

    Availability

    Academic Institutions and Nonprofits only

12. pGEM-HE-Glyco-Venus-bPAC(S27A)

    Plasmid

    #165490

    Purpose

    Xenopus oocyte expression of a photoactivatable adenylyl cyclase derived from bPAC using glycophorin for anchoring to the membrane with reduced dark activity

    Depositor

    Thomas Oertner

    Article

    Yang et al BMC Biol. 2021 Oct 18;19(1):227. doi: 10

    Insert

    Glyco-Venus-bPAC(S27A)-Myc
    

    Use

    Cdna expression, xenopus oocyte

    Tags

    Myc and Venus

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEMHE-NLS-Beta-catenin-S33Y

    Plasmid

    #138537

    Purpose

    For making Beta-catenin-S33Y RNA

    Depositor

    Carmen Williams

    Article

    Gambini et al Dev Cell. 2020 Jun 8;53(5):545-560.e7. d

    Insert

    Beta-catenin
    

    Use

    In vitro transcription

    Tags

    2XFLAG and 2xNLS

    Mutation

    changed serine 33 to tyrosine

    Promoter

    T7

    Available Since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEMT-PT2A-Tdtomato-iRFP670-GFP

    Plasmid

    #111815

    Purpose

    Tricistronic construct: Co-express three fluorescent proteins by P2A and then T2A

    Depositor

    Li Qian

    Article

    Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

    Inserts

    Tdtomato
    iRFP670
    GFP
    

    Use

    Cloning intermediate

    Available Since

    Dec. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEM-cytoBirA-2A-mCherry-SV40pA-FKF

    Plasmid

    #79887

    Purpose

    Donor cassette containing HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    HA-tagged BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    

    Expression

    Bacterial

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. 3155 pGEM-4Z Bcl-2 cDNA G145A

    Plasmid

    #8751

    Depositor

    Stanley Korsmeyer

    Article

    Yin et al Nature 1994 May 26;369(6478):321-3.

    Insert

    Bcl-2 (BCL2 Human)
    

    Expression

    Bacterial

    Mutation

    Glycine 145 mutated to Alanine

    Available Since

    Nov. 14, 2005

    Availability

    Academic Institutions and Nonprofits only

17. pGEMT-T-HSV2.3 TCR alpha chain

    Plasmid

    #74076

    Purpose

    gBT-I TCR a chain cDNA in pGEM-T

    Depositor

    Francis R. Carbone

    Article

    Mueller et al Immunol Cell Biol. 2002 Apr;80(2):156-63

    Insert

    TCR alpha clone HSV2.3 (Tcra Mouse)
    

    Expression

    Bacterial

    Available Since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEM-rat FceRI beta cDNA

    Plasmid

    #8374

    Depositor

    Jean Kinet

    Article

    Kinet et al Proc Natl Acad Sci U S A 1988 Sep;85(17)

    Insert

    rat Fc epsilon RI beta chain (Ms4a2 Rat)
    

    Expression

    Bacterial

    Available Since

    June 7, 2005

    Availability

    Academic Institutions and Nonprofits only

19. pGEM-myr-eGFP-SV40pA-FKF

    Plasmid

    #119866

    Purpose

    Donor cassette containing myr-tagged eGFP and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

    Depositor

    Tatjana Sauka-Spengler

    Article

    Weinberger et al Dev Cell. 2020 Mar 9;52(5):574-590.e6. d

    Insert

    myr-tagged eGFP and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    

    Tags

    2x myristoylation

    Expression

    Bacterial

    Available Since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEM-mouse TCR zeta/2451

    Plasmid

    #11506

    Depositor

    Allan Weissman

    Article

    Weissman et al Science. 1988 Feb 26. 239(4843):1018-21.

    Insert

    TCR zeta (Cd247 Mouse)
    

    Use

    General cloning

    Mutation

    n/a

    Available Since

    March 8, 2006

    Availability

    Academic Institutions and Nonprofits only