We narrowed to 7,122 results for: GFP expression plasmids

1. pBrain-GFP-TACC3KDP∆682-688-shTACC3

   Plasmid

   #69116

   Purpose

   Dual-promoter plasmid to express knockdown-proof human TACC3 (682-688aa ch-TOG binding site deleted) tagged with EGFP along with shRNA to knock down endogenous TACC3 in mammalian cells.

   Depositor

   Stephen Royle

   Article

   Gutierrez-Caballero et al Biol Open. 2015 Jan 16;4(2):170-9. doi:

   Inserts

   Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
   shTACC3
   

   Use

   RNAi

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Deletion of amino acids 682-688 (region containin…

   Promoter

   CMV

   Available Since

   Oct. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAAV2ss-EFS-GFP-synPolyA-U6-sgHTT1-U6-sgCas9

   Plasmid

   #190903

   Purpose

   AAV-KamiCas9 vector expressing expressing thew GFP reporter gene and sgHTT and sgCas9

   Depositor

   Nicole Déglon

   Article

   Duarte et al Mol Ther Methods Clin Dev. 2023 Mar 27;2

   Insert

   GFP, sgHTT and sgCas9 (HTT Human)
   

   Use

   AAV and CRISPR

   Promoter

   EFS and U6

   Available Since

   March 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV-SynI-CreOn-FlpOff-Kir2.1MUT-P2A-EGFP

   Plasmid

   #176279

   Purpose

   Viral vector for co-expression of non-functional Kir2.1 and EGFP in cells expressing Cre AND NOT Flp driven by the human Synapsin promoter.

   Depositor

   Martin Riccomagno

   Article

   Ubina et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

   Insert

   Kir2.1MUT-P2A-EGFP (Kcnj2 Mouse, Synthetic)
   

   Use

   AAV and Cre/Lox

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   GYG to AAA (aa144-146)

   Promoter

   human Synapsin I

   Available Since

   Jan. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA-puro-kozak-scFv-GCN4-HAtag-msfGFP(V206K)-GBI

   Plasmid

   #205162

   Purpose

   express scFv-GCN4-HAtag-msfGFP(V206K)-GBI

   Depositor

   Weirui Ma

   Article

   Hu et al Elife. 2023 Mar 3;12:e82178. doi: 10.755

   Insert

   scFv-GCN4-HAtag-msfGFP(V206K)-GBI
   

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   Oct. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAV hSyn FLEx-loxp Kir2.1-2A-GFP

   Plasmid

   #161574

   Purpose

   Expression of Kir2.1-2A-GFP in a Cre-dependent fashion

   Depositor

   Kevin Beier, Robert Malenka

   Article

   Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

   Insert

   Kir2.1-2A-GFP (Kcnj2 Mouse)
   

   Use

   AAV

   Mutation

   NA

   Available Since

   Nov. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV/mIba1.GFP.WPRE.miR-9.T.miR-129-2-3p.T.SV40pA

   Plasmid

   #190163

   Purpose

   Microglia-targeted transgene expression.

   Depositor

   Hirokazu Hirai

   Article

   Okada et al Commun Biol. 2022 Nov 11;5(1):1224. doi:

   Inserts

   mIba1 promoter, 1.7-kb, microglia-specific promoter
   miR-9-Tx4, 4 repeated miRNA target sites for miR-9
   miR-129-2-3p-Tx4, 4 repeated miRNA target sites for miR-129-2-3p
   

   Use

   AAV

   Available Since

   Nov. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-hSyn-GFP-IRES-hSNCA (A30P)

   Plasmid

   #185720

   Purpose

   AAV expression of GFP and human α-Synuclein with A30P mutation from hSyn promoter

   Depositor

   Jun Ding

   Article

   Albarran et al Nat Neurosci. 2023 Jun;26(6):997-1007. d

   Insert

   synuclein, alpha (SNCA Human)
   

   Use

   AAV

   Mutation

   Changed Ala 30 to Pro

   Promoter

   hSyn

   Available Since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV-syn-FLEX-splitTVA-EGFP-tTA

   Plasmid

   #100798

   Purpose

   helper virus for monosynaptic tracing; to be coinjected with pAAV-TREtight-mTagBFP2-B19G

   Depositor

   Ian Wickersham

   Article

   Liu et al Nature. 2017 Aug 31;548(7669):582-587. d

   Has Service

   AAV1

   Insert

   TVA, EGFP, tet transactivator
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   synapsin

   Available Since

   Oct. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-EF1a-DIO-PSD95-EGFP-WPRE

   Plasmid

   #133785

   Purpose

   Cre-dependent EGFP Fluorescent reporter for overexpressed postsynaptic marker PSD95 in mammalian cell

   Depositor

   Michael Stryker

   Article

   Sun et al Proc Natl Acad Sci U S A. 2019 Oct 22;11

   Insert

   PSD95-EGFP (Dlg4 Rat)
   

   Use

   AAV and Cre/Lox

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   April 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV hSyn FLEx-FRT Kir2.1-2A-GFP

    Plasmid

    #161577

    Purpose

    Expression of Kir2.1-2A-GFP in a Flp-dependent fashion

    Depositor

    Kevin Beier, Robert Malenka

    Article

    Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

    Insert

    Kir2.1-2A-GFP (Kcnj2 Mouse)
    

    Use

    AAV

    Mutation

    NA

    Available Since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Danio alpha A-crystallin 1kb promoter/AcGFP

    Plasmid

    #98096

    Purpose

    aA-crystallin promoter fragment in GFP plasmid to assess activity in larval embryos

    Depositor

    Mason Posner

    Article

    Posner et al PeerJ. 2017 Nov 27;5:e4093. doi: 10.7717

    Insert

    Alpha A-crystallin promoter 1 kb fragment, Danio rerio (cryaa Zebrafish)
    

    Use

    Gfp expressing

    Tags

    GFP

    Promoter

    Danio alpha A-crystallin 1 kb fragment (-1028/-1)

    Available Since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEGFP-C3-PLK4-K41M-S285A-T289A-3xFLAG

    Plasmid

    #69842

    Purpose

    This plasmid encodes kinase dead PLK4 isoform 1 carrying K41M and S285A/T289A mutations with an N-terminal EGFP tag and C-terminal 3xFLAG tag

    Depositor

    Michel Bornens

    Article

    Sillibourne et al Mol Biol Cell. 2010 Feb 15;21(4):547-61.

    Insert

    PLK4 (PLK4 Human)
    

    Tags

    3xFLAG tag and EGFP

    Expression

    Mammalian

    Mutation

    K41M-S285A-T289A

    Promoter

    CMV

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Danio alpha Bb-crystallin 1kb promoter/AcGFP

    Plasmid

    #98098

    Purpose

    aBb-crystallin promoter fragment in GFP plasmid to assess activity in larval embryos

    Depositor

    Mason Posner

    Article

    Posner et al PeerJ. 2017 Nov 27;5:e4093. doi: 10.7717

    Insert

    Alpha Bb-crystallin promoter 1 kb fragment, Danio rerio (cryabb Zebrafish)
    

    Use

    Gfp expressing

    Tags

    GFP

    Promoter

    Danio aBb-crystallin 1 kb fragment (-1074/-1)

    Available Since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Danio alpha Ba-crystallin 3kb promoter/AcGFP

    Plasmid

    #98097

    Purpose

    aBA-crystallin promoter fragment in GFP plasmid to assess activity in larval embryos

    Depositor

    Mason Posner

    Article

    Posner et al PeerJ. 2017 Nov 27;5:e4093. doi: 10.7717

    Insert

    Alpha Ba-crystallin promoter 3 kb fragment, Danio rerio (cryaba Zebrafish)
    

    Use

    Gfp expressing

    Tags

    GFP

    Promoter

    Danio alpha Ba-crystallin 3 kb fragment (-3000/-1)

    Available Since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV.T7-SP6-BC(p11-14)-CAG-EGFP-W3SL-T7

    Plasmid

    #231348

    Purpose

    Single stranded AAV genome with barcodeless T7 RNA polymerase promoter and SP6-driven barcode, for tracking AAV concatemers via SpECTr. Also expresses EGFP from CAG promoter.

    Depositor

    Viviana Gradinaru

    Article

    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

    Insert

    T7-SP6-BC(p11-14)
    

    Use

    AAV

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-hSyn-GFP-IRES-hSNCA (S129D)

    Plasmid

    #185719

    Purpose

    AAV expression of GFP and human α-Synuclein with S129D mutation from hSyn promoter

    Depositor

    Jun Ding

    Article

    Albarran et al Nat Neurosci. 2023 Jun;26(6):997-1007. d

    Insert

    synuclein, alpha (SNCA Human)
    

    Use

    AAV

    Mutation

    Changed Ser 129 to Asp

    Promoter

    hSyn

    Available Since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-hSyn-GFP-IRES-hSNCA (S129A)

    Plasmid

    #185718

    Purpose

    AAV expression of GFP and human α-Synuclein with S129A mutation from hSyn promoter

    Depositor

    Jun Ding

    Article

    Albarran et al Nat Neurosci. 2023 Jun;26(6):997-1007. d

    Insert

    synuclein, alpha (SNCA Human)
    

    Use

    AAV

    Mutation

    Changed Ser 129 to Ala

    Promoter

    hSyn

    Available Since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-hSyn-EGFP-CAAX

    Plasmid

    #208684

    Purpose

    Expresses the membrane-localized enhanced green fluorescent protein EGFP-CAAX in neurons

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    membrane-localized enhanced green fluorescent protein EGFP-CAAX
    

    Use

    AAV

    Promoter

    hSyn

    Available Since

    Oct. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PCDNA3-FlipGFP(TEV cleavage seq) T2A mCherry

    Plasmid

    #124429

    Purpose

    Expresses FlipGFP (TEV cleavage sequence) and T2A mCherry in mammalian cells

    Depositor

    Xiaokun Shu

    Article

    Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

    Insert

    FlipGFP(TEV cleavage seq) T2A mCherry
    

    Expression

    Mammalian

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pet21a-aGFPnb-minimizer-cys-linker-YbbR-(PAS)5-H6

    Plasmid

    #192789

    Purpose

    aGFP nanobody minimizer tagged with cysteine, YbbR and H6 for bacterial expression, purification and labeling

    Depositor

    Jacob Piehler

    Article

    Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

    Insert

    antiGFP nanobody minimizer
    

    Tags

    H6 and ybbR

    Expression

    Bacterial

    Available Since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge