We narrowed to 9,801 results for: pET
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Plasmid#118450PurposeProduces equimolar expression of YPET and mTq2DepositorInsertYPET-T2A-mTurquoise2
ExpressionYeastAvailable SinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW ymYPET-T2A-mTurquoise2
Plasmid#118444PurposeProduces equimolar expression of ymYPET and mTq2DepositorInsertymYPET-T2A-mTurquoise2
ExpressionYeastMutationYPET A206K, F208S, E232L, N235DAvailable SinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET22b-ecDHFR-N23PP/G51PEKN
Plasmid#118583PurposeBacterial expression of E.coli DHFR N23PP and G51PEKN mutantDepositorInsertDHFR
ExpressionBacterialMutationN23 mutated to P23, G51 mutated to P51, a Pro res…PromoterT7Available SinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)-NADPH-LUCID-2 (C50 29 nM)
Plasmid#117909PurposePlasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 29 nMDepositorInsertSNAP_Pro30_[cpNLuc23_24]eDHFR(R98A)
TagsHis-tag and Strep-tagExpressionBacterialMutationR98A on eDHFRPromoterT7Available SinceOct. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pETNKI-his-3C-LIC-Amp
Plasmid#108704PurposeExpression of a His-tagged target protein with 3C protease cleavage site.DepositorTypeEmpty backboneTagsHis-3C protease cleavage siteExpressionBacterialPromoterT7Available SinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET27b-ecDHFR-N23PP/S148A
Plasmid#110829PurposeBacterial expression of E.coli DHFR N23PP/S148A mutantDepositorInsertecDHFR
ExpressionBacterialMutationN23 mutated to P23; a proline residue inserted be…PromoterT7Available SinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28b-NmCas9-Flag H588A
Plasmid#109032PurposeH588A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expressionDepositorInsertCRISPR-associated endonuclease Cas9
TagsFlag TagExpressionBacterialPromoterT7Available SinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-TAF4(827-909)
Plasmid#105620Purposebacterially express TAF4 HFDDepositorInsertTAF4-HFD(827-909) (Taf4 Mouse)
ExpressionBacterialAvailable SinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pETM6-At4CL-PhCHS-MsCHI
Plasmid#73392PurposeExpresses three enzymes to convert phenylpropanoic acids to flavanones in vivoDepositorInsertsAt4CL
PhCHS
MsCHI
ExpressionBacterialPromoterT7Available SinceApril 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pETM6-Pc4CL-PhCHS-CmCHI
Plasmid#73397PurposeExpresses three enzymes to convert phenylpropanoic acids to flavanones in vivoDepositorInsertsPc4CL
PhCHS
CmCHI
ExpressionBacterialPromoterT7Available SinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
M26I DJ-1 TEV site pET15b
Plasmid#60685PurposeRecombinant protein expression of Park7DepositorInsertM26I DJ-1 (PARK7 Human)
Tags6xHisExpressionBacterialMutationM26I and TEV site was inserted (see comments belo…PromoterT7Available SinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
M26L DJ-1 TEV site pET15b
Plasmid#60686PurposeRecombinant protein expression of Park7DepositorInsertM26L DJ-1 (PARK7 Human)
Tags6xHisExpressionBacterialMutationM26L and TEV site was inserted (see comments belo…PromoterT7Available SinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET21a-hAIM2-PYD-GST
Plasmid#51531PurposeExpress GST-tagged PYD of hAIM2 in bacteriaDepositorInsertAIM2 (AIM2 Human)
TagsGST and T7ExpressionBacterialMutationdeletion of amino acids 102-343Promoterlac operatorAvailable SinceMarch 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
p231-pOsAct1::hpt-pZmUbi::BdPetC-p35S::mTurquoise
Plasmid#129650PurposeRieske FeS overexpressionDepositorInsertCDS of PetC gene encoding for the Rieske FeS subunit
ExpressionPlantMutationCodon modified for Golden GatePromoterpZmUbiAvailable SinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21-10XHis-GST-HRV-Her1-dL5-Her1
Plasmid#73217PurposeBacterial expression vector for Z1907-dL5(E52D)-Z1907 affibody fusion protein (AFA) with N-terminal His10, GST and HRV3C cleavage site (MBIC5, dL5**, FAP, AffiFAP)DepositorInsert10XHis-GST-HRV-Her1-dL5-Her1 (EGFR Schistosoma japonicum, Human, Synthetic)
Tags10XHis-GST-HRV and The Her1 Affibody and dL5 FAP …ExpressionBacterialMutationThe dL5** FAP is E50D, L89S, also known as E52D/L…PromoterT7Available SinceMarch 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-HIS-TagGFP2-TEV-GGG-ELP(40nm)-Cys
Plasmid#90474PurposeElastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]2, with N-Terminal TagGFP2 and TEV-cleavage site, leaving an N-terminal Sortase Recognition Sequence GGG and a C-terminal CysteineDepositorInsertTagGFP2-TEV-GGG-ELP(40nm)-Cys
TagsCysteine and Sortase-TagExpressionBacterialPromoterT7Available SinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
Affibody EGFR:1907.BirA*-pET301/NT-DEST
Plasmid#174691PurposePlasmid encoding for the bacterial expression of a bispecific coding for the anti-EGFR affibody ZEGFR:1907 fused to the mutated form of BirA enzyme, BirA*DepositorInsertAnti-EGFR affibody ZEGFR:1907 fused to mutated form of BirA, BirA*
TagsHIS tagExpressionBacterialMutationR118G mutation in BirAPromoterT7 promoterAvailable SinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET29b-AviTag-eGFP-dspB(E184Q)-6xHis
Plasmid#175784PurposePlasmid for overexpression of recombinant AviTagged, His-tagged fusion protein eGFP-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.DepositorInsertDispersin B
TagsAviTag, Hexahistidine tag, and eGFPExpressionBacterialMutationE184QPromoterT7Available SinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
BPV00554 pET21GG2-His(6)-BECN(248-450)
Plasmid#136685PurposeExpresses the Beclin-1 ECD/BARA domain (a.a. 248-450) with a N-terminal hexa-Histidine tagDepositorInsertBeclin-1 ECD/BARA (BECN1 Human)
TagsHexa-HistadineExpressionBacterialMutationDNA optimized for E. coli expressionPromoterT7Available SinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJAG98-mTurquoise2-FHA2-AURKB substrate-YPet
Plasmid#83286Purposeuntargeted Aurora B FRET sensor with mTurquoise2 and YPet FRET pairDepositorInsertmTurquoise2-FHA2-AURKB substrate-YPet
UseRetroviralExpressionMammalianAvailable SinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only