We narrowed to 5,366 results for: pcDNA3.1+

Showing: 3801 - 3820 of 5366 results

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YFP Rab4DN

Plasmid

#99019

Purpose

Localization and visualization of the dominant negative form of Rab4

Depositor

Narasimhan Gautam

Article

O'Neill et al Dev Cell. 2018 Jun 15. pii: S1534-5807(1

Insert

Rab4 DN (Rab4 Fly, Synthetic)

Tags

YFP

Expression

Mammalian

Mutation

S22N Dominant negative

Promoter

CMV

Available Since

June 28, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-ERK2 L198A L232A

Plasmid

#8978

Depositor

John Blenis

Article

Dimitri et al Curr Biol. 2005 Jul 26. 15(14):1319-24.

Insert

ERK2 (Mapk1 Rat)

Tags

HA

Expression

Mammalian

Mutation

L198A, L232A

Available Since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only
pCDNA-CRY2-mCh-superPLDx30-P2A-CIBN-CAAX

Plasmid

#188992

Purpose

A plasmid for dual expression of CRY2-mCh-superPLDx30 (an engineered PLD mutant with 30 times higher activity than the wild-type) and plasma membrane-tagged CIBN

Depositor

Jeremy Baskin

Article

Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

Insert

PLD

Tags

CAAX, CIBN, CRY2, P2A, and mCherry

Expression

Mammalian

Mutation

K109R, P245A, G328S, G381V, G429D

Promoter

CMV

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pER-eZinCh-2

Plasmid

#70009

Purpose

expresses ER-eZinCh-2 Zn2+ FRET sensor in the ER of mammalian cells

Depositor

Maarten Merkx

Article

Hessels et al ACS Chem Biol. 2015 Sep 18;10(9):2126-34

Insert

ER-eZinCh-2

Tags

KDEL sequence and PPI signal

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pmyc-GFP-TNRC6A-NES-mut

Plasmid

#42001

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

TNRC6A (TNRC6A Human)

Tags

EGFP and Myc

Expression

Mammalian

Mutation

F951A, F955A, I958A, F960A

Promoter

CMV

Available Since

March 6, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3 RL3Sc

Plasmid

#109365

Purpose

Human rod opsin chimera with intracellular loop 3 from Scallop opsin with 1D4 tag

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

Rod opsin chimera with Scallop Opsin2 Loop 3 (RHO, LOC110451845 Patinopecten yessoensis)

Tags

1D4

Expression

Mammalian

Promoter

CMV

Available Since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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FLAG-YL1

Plasmid

#15333

Depositor

Joan Conaway, Ronald Conaway

Article

Cai et al J Biol Chem. 2005 Apr 8. 280(14):13665-7

Insert

transcription factor-like 1 (VPS72 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only
FKBP-CD28TMD-NTEVp(WT)

Plasmid

#137826

Purpose

MESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal WT TEV protease

Depositor

Joshua Leonard

Article

Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

Insert

FKBP-CD28TMD-NTEVp(WT)

Tags

3x FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA_DiCre_60.F2

Plasmid

#60208

Purpose

Expresses a fusion protein of FRB-linker peptide 2- Cre(60-343)

Depositor

Jean-Paul Herman

Article

Jullien et al Nucleic Acids Res. 2003 Nov 1;31(21):e13

Insert

Cre recombinase (CD207 Synthetic, Bacteriophage P1, Human)

Tags

FRAP and Nuclear localisation signal

Expression

Mammalian

Mutation

amino acids 2021-2112 of FRAP with a T2098L mutat…

Promoter

CMV

Available Since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HCMVgL-FLAG (Merlin)

Plasmid

#136450

Purpose

Mammalian expression plasmid for HCMV glycoprotein L (Merlin strain) with FLAG tag

Depositor

Erik Procko

Article

Park et al PLoS Pathog. 2020 Jun 19;16(6):e1008647.

Insert

glycoprotein L (UL115 Human betaherpesvirus 5)

Tags

Short SGGSG linker and FLAG tag

Expression

Mammalian

Mutation

Codon-optimized for human cell expression.

Promoter

CMV

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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Dr-EGFR

Plasmid

#184004

Purpose

Dr-EGFR expression in mammalian cells

Depositor

Vladislav Verkhusha

Article

Leopold et al J Mol Biol. 2020 Jun 12;432(13):3749-376

Insert

Dr-EGFR (EGFR Human)

Tags

Deinococcus radiodurans bacterial phytochrome

Expression

Mammalian

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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Bongwoori

Plasmid

#63720

Purpose

Consists of the triple mutant voltage sensing domain and the optimized linker between the S4 transmembrane domain and the super ecliptic pHlorin A227D

Depositor

Bradley Baker

Article

Piao et al J Neurosci. 2015 Jan 7;35(1):372-85. doi

Insert

Bongwoori

Tags

Super ecliptic pHlorin A227D

Expression

Mammalian

Promoter

CMV

Available Since

July 28, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA-NES-ZapCmR2

Plasmid

#59015

Purpose

Cytosolic zinc-binding FRET biosensor

Depositor

Amy Palmer

Article

Miranda et al PLoS One. 2012;7(11):e49371. doi: 10.137

Insert

NES-ZapCmR2 genetically encoded cytosolic Zn(II) sensor

Tags

Nuclear Exclusion Signal

Expression

Mammalian

Mutation

See comment

Promoter

CMV

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
mGFAP-Halo

Plasmid

#169474

Purpose

the host vector was obtained by substitution of the EGFP tag from a pEGFP-N3-mGFAP plasmid for a HaloTag using BamHI and NotI restriction sites

Depositor

Federico Herrera

Article

Letra-Vilela et al Experimental Results (2020), Volume 1, e

Insert

GFAP (Gfap Mouse)

Tags

HaloTag

Expression

Mammalian

Mutation

Changed R 236 to H

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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pmyc-GFP-TNRC6A-NLS-mut

Plasmid

#42000

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

TNRC6A (TNRC6A Human)

Tags

EGFP and Myc

Expression

Mammalian

Mutation

R930A, R931A, R932A, R934A

Promoter

CMV

Available Since

March 6, 2013

Availability

Academic Institutions and Nonprofits only

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PA-RL-mCherry

Plasmid

#78861

Purpose

DULIP negative control (bait) to exclude unspecific background binding

Depositor

Erich Wanker

Article

Trepte et al J Mol Biol. 2015 Oct 23;427(21):3375-88.

Insert

mCherry

Use

Expression vector

Tags

Protein A, Renilla luciferase

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2016

Availability

Academic Institutions and Nonprofits only

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pCDNA-CRY2-mCh-superPLDx50-P2A-CIBN-CAAX

Plasmid

#188993

Purpose

A plasmid for dual expression of CRY2-mCh-superPLDx50 (an engineered PLD mutant with 50 times higher activity than the wild-type) and plasma membrane-tagged CIBN

Depositor

Jeremy Baskin

Article

Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

Insert

PLD

Tags

CAAX, CIBN, CRY2, P2A, and mCherry

Expression

Mammalian

Mutation

D201G, A258T, K327R, G328S, G381V, G429D, T450A

Promoter

CMV

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA-HA-CAML

Plasmid

#22284

Depositor

Erik Lillehoj

Article

Guang et al Int J Biochem Cell Biol. 2009 Jun . 41(6

Insert

calcium-modulating ligand (CAMLG Human)

Tags

HA

Expression

Mammalian

Available Since

Dec. 2, 2009

Availability

Academic Institutions and Nonprofits only
pCDNA-SCW-HHL

Plasmid

#190634

Purpose

Mammalian expression of genetically encoded calcium indicator SCW-HHL

Depositor

Robert Campbell

Article

Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

Insert

SCW-HHL

Expression

Mammalian

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pSSA-PMG (pXK-1649)

Plasmid

#208857

Purpose

SSA-base selectable traffic light reporter (TLR) for helping to screen gene-editing positive cells. Key Construct: CMV-PuroR.L-mCherry-PuroR.R-GFP.

Depositor

Kun Xu

Article

Lyu et al Int J Biol Macromol. 2023 Jul 15;243:124

Type

Empty backbone

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

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