Addgene

We narrowed to 11,205 results for: SHA

Showing: 5701 - 5730 of 11205 results

Results per page:

Sort results by:

pFE-Reu-FI-prk

Plasmid

#162696

Purpose

Expresses R. eutropha Form IC rubisco and S. elongatus Prk

Depositor

David Savage

Article

Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

Insert

R. eutropha CbbLS and S. elongatus Prk

Use

Tags

N terminal 6x His tag on PRK

Expression

Bacterial

Mutation

Promoter

PLtet0-1 promoter

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAM232

Plasmid

#226654

Purpose

pET24b-p97-FLAG

Depositor

Andreas Martin

Article

Blythe et al Structure. 2019 Dec 3;27(12):1820-1829.e

Insert

p97 (VCP Human)

Use

Tags

FLAG

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
mTagBFP2-H3.3-N-14

Plasmid

#55302

Purpose

Localization: Nucleus/Histones, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

H3.3 (H3f3b Mouse)

Use

Tags

mTagBFP2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pZac2.1-GfaABC1D-GAT3-HA-mCherry

Plasmid

#117722

Purpose

Expresses a GABA transporter GAT-3 (Slc6a11)

Depositor

Baljit Khakh

Article

Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

Insert

Slc6a11 (Slc6a11 Mouse)

Use

AAV

Tags

HA and mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFE-Rs-FI-prk

Plasmid

#162697

Purpose

Expresses R. spaeroides Form IC rubisco and S. elongatus Prk

Depositor

David Savage

Article

Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

Insert

R. spaeroides Form IC rubisco and S. elongatus Prk

Use

Tags

N terminal 6x His tag on PRK

Expression

Bacterial

Mutation

Promoter

PLtet0-1 promoter

Available since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLEX303 GFP-PIK3C2B

Plasmid

#162001

Purpose

Lentiviral expression vector containing PIK3C2B tagged with GFP

Depositor

David Bryant

Article

Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

Insert

PIK3C2B (PIK3C2B Human)

Use

Lentiviral

Tags

eGFP

Expression

Mutation

siRNA resistance to siRNA#2 with 4 silent mutatio…

Promoter

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pFUW-tetO-Relb

Plasmid

#139810

Purpose

doxycycline-inducible expression of mouse Relb in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

Insert

Relb (Relb Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE-mCMV

Available since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHiUGE smFP-V5 donor ORF2

Plasmid

#200382

Purpose

Tagging endogenously expressed proteins with "spaghetti monster" smFP-V5, C-terminal

Depositor

Scott Soderling

Article

Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

Insert

spaghetti monster smFP-V5 (ref: PMID: 25915120, Addgene plasmid # 59758 )

Use

Tags

Expression

Mammalian

Mutation

NA

Promoter

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
PLKO.1-G3BP1-3'UTR

Plasmid

#136038

Purpose

G3BP1 shRNA (Targeting 3'UTR) inserted into the PLKO.1 plasmid (GCCTGTAAGAAATACAGGATT)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

G3BP1 (G3BP1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
hKv2.1-pEGFP-N1

Plasmid

#111532

Purpose

Expresses human KCNB1 in mammalian cells for imaging

Depositor

Federico Sesti

Article

Yu et al Cell Death Dis. 2017 Apr 6;8(4):e2737. d

Insert

human Kv2.1 (KCNB1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pKP3915

Plasmid

#113335

Purpose

Beta-estradiol inducible expression vector; Z4 promoter-CYC1 terminator; artificial transcription factor under ACT1 promoter

Depositor

Suzanne Sandmeyer

Article

Patterson et al Genome Res. 2019 Aug;29(8):1298-1309. do

Insert

Artificial Transcription Factor (McIsaac et al, 2013)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
PITPbeta-GFP

Plasmid

#58274

Purpose

mammalian expression of GFP-PITP beta

Depositor

Shamshad Cockcroft

Article

Morgan et al Biochem J. 2006 Sep 15;398(3):411-21.

Insert

PITP beta (PITPNB Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 21, 2014

Availability

Academic Institutions and Nonprofits only
pC003 - LshC2C2 locus into pACYC184 for spacer cloning

Plasmid

#79152

Purpose

LshC2c2 locus in pACYC184 with BsaI sites for spacer cloning

Depositor

Feng Zhang

Article

Abudayyeh et al Science. 2016 Jun 2. pii: aaf5573.

Insert

LshC2c2 locus

Use

Tags

Expression

Mutation

*

Promoter

Available since

June 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
cyto-YFP-FKBPx5

Plasmid

#103777

Purpose

expression of iPOLYMER component in mammalian cells; has nuclear exclusion sequence

Depositor

Takanari Inoue

Article

Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

Insert

cyto-YFP-FKBPx5

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
cyto-CFP-FRBx5

Plasmid

#103776

Purpose

expresses iPOLYMER component in mammalian cells

Depositor

Takanari Inoue

Article

Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

Insert

cyto-CFP-FRBx5

Use

Tags

ECFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pFUW-tetO-Nfil3

Plasmid

#139815

Purpose

doxycycline-inducible expression of mouse Nfil3 in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

Insert

Nfil3 (Nfil3 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE-mCMV

Available since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHDR-CDKN2A-Ex2-CMV-EGFP

Plasmid

#110734

Purpose

Recombination template for replacing CDKN2A Exon2 with CMV-EGFP

Depositor

Robert Judson-Torres

Article

Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

Insert

EGFP

Use

Other

Tags

Expression

Mutation

Promoter

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
DsRed-rab9 DN

Plasmid

#12676

Depositor

Richard Pagano

Article

Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

Insert

RAB9 (Homo sapiens) (RAB9A Human)

Use

Tags

DsRed and HA

Expression

Mammalian

Mutation

changed serine 21 to asparagine

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV CAGG eGFP

Plasmid

#107707

Purpose

express eGFP in variety of cell types

Depositor

Troy Margrie

Article

Velez-Fort et al Neuron. 2018 Apr 4;98(1):179-191.e6. doi

Insert

eGFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAGG

Available since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
mTagBFP2-Calnexin-N-14

Plasmid

#55278

Purpose

Localization: Endoplasmic Reticulum, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

Calnexin (CANX Human)

Use

Tags

mTagBFP2

Expression

Mammalian

Mutation

I474N in Calnexin

Promoter

CMV

Available since

Oct. 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pRH2502

Plasmid

#84379

Purpose

Expression of dcas9 D10A H840A from a TetR-regulated uvtetO promoter

Depositor

Robert Husson

Article

Singh et al Nucleic Acids Res. 2016 Jul 12. pii: gkw

Insert

dcas9

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Aspartate at position 10 changed to Alanine, Hist…

Promoter

uv15tetO

Available since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pCB

Plasmid

#162699

Purpose

Expresses pHnCB10 derived carboxysome operon and prk

Depositor

David Savage

Article

Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

Insert

pHnCB10 derived carboxysome operon, prk

Use

Tags

Expression

Bacterial

Mutation

Promoter

PLtet0-1 promoter

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
IBMc396

Plasmid

#161954

Purpose

Demonstrates chemically induced dimmerization of acVHH. Expression plasmid for split, evolved T7 RNA polymerase N-lobe fused to acVHH

Depositor

Baojun Wang

Article

Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

Insert

pSEVA321-PBAD(SapI-)-B30-His-T7RNAP(29-1)-15aa-acVHH-T

Use

Tags

Expression

Bacterial

Mutation

Promoter

PBAD

Available since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-hSyn-LMO11 (GeNL_SS-ChRmine)

Plasmid

#213034

Purpose

fusion protein of mNeonGreen, SSLuc, and ChRmine for bioluminescent optogenetics

Depositor

Ute Hochgeschwender

Article

Slaviero et al Neurophotonics. 2024 Apr;11(2):024208. d

Insert

mNeonGreen, ISN-IE, ChRmine

Use

AAV

Tags

Gaussia luciferase signal sequence

Expression

Mutation

Promoter

hSyn

Available since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pUCXMG

Plasmid

#204409

Purpose

Expression plasmid for cloning of FatI digested metagenomic DNA into a compatible NcoI site

Depositor

David Ackerley

Article

Rich et al bioRxiv. 2023 Mar 25:2023.03.24.534183.

Type

Empty backbone

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

tac

Available since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR-FOXP2

Plasmid

#47053

Purpose

Expresses forkhead box P2

Depositor

Huda Zoghbi

Article

Sakai et al Sci Transl Med. 2011 Jun 8;3(86):86ra49.

Insert

Forkhead Box P2 (FOXP2 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Oct. 24, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3-Nuc-ExRai-AMPKAR

Plasmid

#192450

Purpose

ExRai-AMPKAR targeted to the nucleus.

Depositor

Jin Zhang

Article

Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

Insert

ExRai-AMPKAR-NLS

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pFA6a-kanMX6-PGAL1-3HA

Plasmid

#41608

Depositor

John Pringle

Article

Longtine et al Yeast. 1998 Jul;14(10):953-61.

Insert

GAL1 promoter (GAL1 Budding Yeast)

Use

Yeast genomic targeting

Tags

3xHA, A. gossypii translation elongation factor 1…

Expression

Mutation

Promoter

Available since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
dCBE4-gam

Plasmid

#124449

Purpose

Expresses dead Cas9 (dCas9)-CBE4-gam

Depositor

George Church

Article

Smith et al Nucleic Acids Res. 2020 May 21;48(9):518

Insert

dCBE4-gam

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

D10A, H840A

Promoter

Available since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLEX301-TagRFP

Plasmid

#162035

Purpose

Tagged vector control

Depositor

David Bryant

Article

Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

Type

Empty backbone

Use

Lentiviral

Tags

RFP

Expression

Mutation

Promoter

Available since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge