We narrowed to 4,564 results for: HRE

1. pGL3 GATA Luc

   Plasmid

   #85695

   Purpose

   Luciferase reporter containing three repeats of the GATA consensus site

   Depositor

   Licio Collavin, Giannino Del Sal

   Article

   Collavin et al Proc Natl Acad Sci U S A. 2004 Jun 15;10

   Insert

   3xGATA and minimal MT promoter
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   3xGATA and minimal MT promoter

   Available since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

2. pCH1 - pBait-empty

   Plasmid

   #174663

   Purpose

   arabinose-induced synthesis of an RNA containing a single MS2 hairpin. Used as a three-hybrid control. For cloning purposes, see plasmid #174662.

   Depositor

   Katherine Berry

   Article

   Pandey et al Nucleic Acids Res. 2020 May 7;48(8):4507

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEYFP-N1-HsSmg7_H

   Plasmid

   #146476

   Purpose

   Mammalian Expression of HsSmg7

   Depositor

   Elisa Izaurralde

   Article

   Unterholzner et al Mol Cell. 2004 Nov 19. 16(4):587-96.

   Insert

   HsSmg7 (SMG7 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   one non silent mutation G2560A (V854I) and three …

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEYFP-N1-HsSmg5_H

   Plasmid

   #146474

   Purpose

   Mammalian Expression of HsSmg5

   Depositor

   Elisa Izaurralde

   Article

   Unterholzner et al Mol Cell. 2004 Nov 19. 16(4):587-96.

   Insert

   HsSmg5 (SMG5 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   three non silent mutation A254G (K85R), T980C (L3…

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pEG476

   Plasmid

   #40198

   Depositor

   Geraldine Seydoux

   Article

   Griffin et al Cell. 2011 Sep 16;146(6):955-68.

   Insert

   Mex-5 (S404A) (mex-5 Nematode)
   

   Use

   Tags

   Dendra2 PAFP (sequence optimized for worm express…

   Expression

   Worm

   Mutation

   S404A

   Promoter

   pie-1

   Available since

   Oct. 4, 2012

   Availability

   Academic Institutions and Nonprofits only

6. Venus-Bik-DD-pEGFP-C1

   Plasmid

   #177429

   Purpose

   To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, phosphomimetic mutant. T33D and S35D mutations made to Addgene #166737.

   Depositor

   David Andrews

   Insert

   Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   Threonine 33 and Serine 35 replaced with aspartic…

   Promoter

   CMV

   Available since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAc5.1B-EGFP-DmFMR1_B

   Plasmid

   #145920

   Purpose

   Insect Expression of DmFMR1

   Depositor

   Elisa Izaurralde

   Insert

   DmFMR1 (Fmr1 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Two amino acid mutations (T214I and N501S) and th…

   Promoter

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. YFP-Rac2(N17)

   Plasmid

   #11398

   Depositor

   Joel Swanson

   Article

   Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

   Insert

   ras-related C3 botulinum toxin substrate 2 (RAC2 Human)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Obtained with Threonine 17 changed to Asparagine.

   Promoter

   Available since

   Feb. 21, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV-pOTC-3H-GFP

   Plasmid

   #205725

   Purpose

   Expression of GFP fused with pOTC-3H, a leader peptide from mitochondrial matrix enzyme ornithine transcarbamylase and a thermostable three helix-bundle

   Depositor

   Randy Schekman

   Article

   Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

   Insert

   pOTC-3H-GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCDNA3 10 mut B-Myb

    Plasmid

    #25966

    Depositor

    Rob Lewis

    Article

    Johnson et al J Biol Chem. 1999 Dec 17. 274(51):36741-

    Insert

    B-Myb (Mybl2 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    changed threonines 267, 408, 497, 519, 522 and 52…

    Promoter

    Available since

    Sept. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

11. pGK415-­rtetTAK8N, L131L

    Plasmid

    #165969

    Purpose

    Expresses rTetTA (K8N, L131L) mutant

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

    Insert

    tetR
    

    Use

    Synthetic Biology

    Tags

    three copies of VP16 (VP48)

    Expression

    Yeast

    Mutation

    Promoter

    pPGK1

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAc5.1B-lambdaN-HA-DmFMR1_B

    Plasmid

    #145940

    Purpose

    Insect Expression of DmFMR1

    Depositor

    Elisa Izaurralde

    Insert

    DmFMR1 (Fmr1 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Two amino acid mutations (N45D and G558R) and thr…

    Promoter

    Available since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. p3PA-Puro-iTk

    Plasmid

    #109067

    Purpose

    inserts three polyA sites to end transcription of a targetted gene and a puromycin cassette for selection

    Depositor

    Eric Lander

    Article

    Engreitz et al Nature. 2016 Nov 17;539(7629):452-455. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLenti-CaMKIIa-hChR2(C128T)-EYFP-WPRE

    Plasmid

    #20295

    Purpose

    Lentiviral expression of ChR2 (C128T) Step Function Opsin (SFO) fused to EYFP, for bi-stable optogenetic excitation

    Depositor

    Karl Deisseroth

    Article

    Berndt et al Nat Neurosci. 2009 Feb . 12(2):229-34.

    Insert

    channelrhodopsin-2
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    changed Cysteine 128 to Threonine

    Promoter

    Available since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pR184

    Plasmid

    #47952

    Purpose

    rtTA positive feedback loop with three tet operators in the GAL1 promoter

    Depositor

    Attila Becskei

    Article

    Hsu et al Nat Commun. 2012 Feb 21;3:682. doi: 10.1

    Insert

    rtTA
    

    Use

    Tet inducible expression

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Gal1

    Available since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. BMPR2

    Plasmid

    #38972

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    BMPR2 (BMPR2 Human)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pENTR-fishRab8A[T22N]

    Plasmid

    #24906

    Depositor

    Maxence Nachury

    Article

    Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

    Insert

    fishRab8A
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Changed Threonine 22 to Asparagine

    Promoter

    Available since

    July 7, 2010

    Availability

    Academic Institutions and Nonprofits only

18. p413GPD-hTPI Ile170Thr

    Plasmid

    #50721

    Purpose

    expression of the human TPI Ile170Thr allele in yeast

    Depositor

    Markus Ralser

    Article

    Gruning et al Open Biol. 2014 Mar 5;4(3):130232. doi:

    Insert

    Triosephosphate isomerase 1 (TPI1 Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Isoleucine 170 to Threonine

    Promoter

    GPD

    Available since

    Feb. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. M27 NacMS>luc

    Plasmid

    #17177

    Depositor

    Randall Moon

    Article

    Dorsky et al Genes Dev. 2000 Jan 15;14(2):158-62.

    Insert

    nacre promoter (mitfa Zebrafish)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mutation

    All three Tcf/Lef sites are mutated

    Promoter

    Available since

    Feb. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

20. piHGpd/T101I

    Plasmid

    #1112

    Depositor

    Susan Lindquist

    Article

    Nathan et al Proc Natl Acad Sci U S A 1999 Feb 16;96(

    Insert

    HSP82 (HSP82 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    HSP82 with threonine 101 changed to isoleucine; r…

    Promoter

    Available since

    Jan. 6, 2005

    Availability

    Academic Institutions and Nonprofits only