We narrowed to 7,533 results for: ski

1. pUAST YFP Rab8 DN

   Plasmid

   #58451

   Purpose

   expression of drosophila YFP Rab8 dominant negative mutant

   Depositor

   Matthew Scott

   Article

   Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

   Insert

   Rab8 (Rab8 Fly)
   

   Tags

   YFP

   Expression

   Insect

   Mutation

   T22N (dominant negative)

   Promoter

   GAL4

   Available Since

   July 21, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pUAST YFP Rab2 DN

   Plasmid

   #58445

   Purpose

   expression of drosophila YFP-Rab2 dominant negative mutant

   Depositor

   Matthew Scott

   Article

   Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

   Insert

   Rab2 (Rab2 Fly)
   

   Tags

   YFP

   Expression

   Insect

   Mutation

   S20N (dominant negative)

   Promoter

   GAL4

   Available Since

   July 21, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pUAST YFP Rab2 CA

   Plasmid

   #58443

   Purpose

   expression of drosophila YFP-Rab2 constitutively active mutant

   Depositor

   Matthew Scott

   Article

   Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

   Insert

   Rab2 (Rab2 Fly)
   

   Tags

   YFP

   Expression

   Insect

   Mutation

   Q65L (constitutive active)

   Promoter

   GAL4

   Available Since

   July 21, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pET14b-PITPalpha K61A

   Plasmid

   #58258

   Purpose

   bacterial expression of human PITP alpha K61A

   Depositor

   Shamshad Cockcroft

   Article

   Tilley et al Structure. 2004 Feb;12(2):317-26.

   Insert

   PITP alpha (PITPNA Human)
   

   Tags

   HIS

   Expression

   Bacterial

   Mutation

   K61A (abolishes PI binding)

   Promoter

   T7

   Available Since

   July 15, 2014

   Availability

   Academic Institutions and Nonprofits only

5. Danio rerio cryaa V62T

   Plasmid

   #47630

   Purpose

   Recombinant protein production

   Depositor

   Mason Posner

   Article

   Posner et al PLoS One. 2012;7(3):e34438. doi: 10.1371

   Insert

   Alpha A-crystallin (cryaa Zebrafish)
   

   Expression

   Bacterial

   Mutation

   Changed Valine 62 to Threonine

   Promoter

   T7

   Available Since

   Oct. 21, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. Danio rerio cryaa T147V

   Plasmid

   #47628

   Purpose

   Recombinant protein production

   Depositor

   Mason Posner

   Article

   Posner et al PLoS One. 2012;7(3):e34438. doi: 10.1371

   Insert

   Alpha A-crystallin (cryaa Zebrafish)
   

   Expression

   Bacterial

   Mutation

   Changed Theronine 147 to Valine

   Promoter

   T7

   Available Since

   Sept. 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. Danio rerio cryaa C143S

   Plasmid

   #47619

   Purpose

   Recombinant protein production

   Depositor

   Mason Posner

   Article

   Posner et al PLoS One. 2012;7(3):e34438. doi: 10.1371

   Insert

   Alpha A-crystallin (cryaa Zebrafish)
   

   Expression

   Bacterial

   Mutation

   Cysteine 143 to Serine

   Promoter

   T7

   Available Since

   Sept. 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLEX_970_puro_DEST_V5_TLOC1 (220aa)

   Plasmid

   #45287

   Depositor

   William Hahn

   Article

   Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

   Insert

   TLOC1 (SEC62 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Available Since

   May 22, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pUASp YFP Rab14 DN

   Plasmid

   #37715

   Depositor

   Matthew Scott

   Article

   Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

   Insert

   Rab14 (Rab14 Fly)
   

   Use

   Drosophila

   Tags

   YFP

   Expression

   Insect

   Mutation

   S49N (dominant negative)

   Promoter

   GAL4

   Available Since

   March 13, 2013

   Availability

   Academic Institutions and Nonprofits only

10. MET FLAG72QDProCFPp303

    Plasmid

    #15590

    Depositor

    Susan Lindquist

    Article

    Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    htt 72Q Delta Pro (HTT Human)
    

    Tags

    CFP and FLAG

    Expression

    Yeast

    Mutation

    Proline-rich region removed

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

11. pmCell-Free_KA2_ZIKV NS2B

    Plasmid

    #145364

    Purpose

    Cell-free/mammalian expression vector for Zika virus NS2B protein with N-term mCherry

    Depositor

    Rob Parton

    Article

    Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

    Insert

    ZIKV NS2B
    

    Tags

    8xHis and mCherry

    Expression

    Bacterial

    Promoter

    T7- CMV/CAG

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRS415-TEF1pr-MFA-NFAPoptim

    Plasmid

    #221090

    Purpose

    FAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag) + MFA1 signal sequence to help target constructs to the ER

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMitoTimer

    Plasmid

    #52659

    Purpose

    Expresses mitochondrial targeted DsRed1-E5 as reporter for mitochondrial content, structure, oxidative stress and damage in vitro and in vivo

    Depositor

    Zhen Yan

    Article

    Laker et al J Biol Chem. 2014 Apr 25;289(17):12005-1

    Insert

    DsRed1-E5
    

    Use

    Dsred

    Tags

    human cytochrome c oxidase subunit VIII mitochond…

    Promoter

    CMV

    Available Since

    April 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. GFP-OMP25

    Plasmid

    #141150

    Purpose

    Fluorescent labeling of the outer mitochondrial membrane (OMM) in mammalian cells

    Depositor

    Gia Voeltz

    Article

    Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    OMP25 (Synj2bp Rat)
    

    Tags

    AcGFP

    Expression

    Mammalian

    Mutation

    OMP25 c terminus from addgene plasmid #69598

    Promoter

    CMV

    Available Since

    Aug. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDY1330 STITCHR pCMV-nCas9-XTEN-v170_R2Tocc

    Plasmid

    #234826

    Purpose

    STITCHR R2Tocc editor

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Fell et al Nature. 2025 Apr 9. doi: 10.1038/s41586-

    Insert

    nCas9h840a-xten-R2Tocc
    

    Use

    CRISPR

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDH-hMALAT1

    Plasmid

    #118580

    Purpose

    to overexpress human MALAT1 in mammalian cells

    Depositor

    Jianjun Zhao

    Article

    Hu et al Leukemia. 2018 Mar 22. pii: 10.1038/s413

    Insert

    MALAT1 (MALAT1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    MSCV

    Available Since

    Oct. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX333

    Plasmid

    #64073

    Purpose

    Vector for tandem expression of two sgRNAs from two independent U6 promoters. Cas9 is expressed by Cbh promoter.

    Depositor

    Andrea Ventura

    Article

    Maddalo et al Nature. 2014 Dec 18;516(7531):423-7. doi

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Tags

    3XFLAG-Cas9

    Expression

    Mammalian

    Promoter

    CBh; U6

    Available Since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTagRFP658-N1

    Plasmid

    #178974

    Purpose

    Expresses TagRFP658 in mammalian cells

    Depositor

    Kiryl Piatkevich

    Article

    Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

    Insert

    TagRFP658
    

    Tags

    no

    Expression

    Mammalian

    Mutation

    NO

    Promoter

    CMV

    Available Since

    Aug. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. lentiCRISPRi(v2)-Blast

    Plasmid

    #170068

    Purpose

    Expresses dCas9 repressor KRAB-dCas9-MeCP2 and blasticidin resistance

    Depositor

    Neville Sanjana

    Article

    Morris et al Science. 2023 May 19;380(6646):eadh7699.

    Insert

    KRAB-dCas9-MeCP2
    

    Use

    CRISPR and Lentiviral

    Tags

    2A and 2xHA tag

    Expression

    Mammalian

    Promoter

    EFS-NS

    Available Since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. PGK-H2BeGFP

    Plasmid

    #21210

    Depositor

    Mark Mercola

    Article

    Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

    Insert

    Phosphoglycerate kinase promoter (PGK1 Human)
    

    Use

    Lentiviral

    Tags

    H2B-eGFP

    Available Since

    July 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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