We narrowed to 9,512 results for: try

1. ACE2-Fc

   Plasmid

   #164222

   Purpose

   Expresses soluble , dimeric Fc-His fusion protein for the human ACE2 extracellular domain, along with dTomato reporter

   Depositor

   Sriram Neelamegham

   Article

   Yang et al Elife. 2020 Oct 26;9. pii: 61552. doi: 1

   Insert

   ACE2 extracellular domain (ACE2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pENTR221-IL6.Pro

   Plasmid

   #79491

   Purpose

   MultiSite Gateway entry clones, first fragment, (attL1, attR5) Human IL6 promoter

   Depositor

   Peter Jon Nelson

   Article

   Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

   Insert

   Il6 Promoter (IL6 Human, Synthetic)
   

   Use

   Multisite gateway entry clone

   Promoter

   IL6 Promoter

   Available Since

   May 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKK-CyOFP1-TEV

   Plasmid

   #105782

   Purpose

   Expression of your protein of interest in fusion with orange fluorescent protein at the N-terminus (cleavable by TEV) (PMID: 27240196). CyOFP1 is useful for multi-channel imaging.

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   CyOFP1-TEV

   Expression

   Mammalian

   Available Since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKK-mClover3-TEV

   Plasmid

   #105778

   Purpose

   Expression of your protein of interest in fusion with green fluorescent protein at the N-terminus (cleavable by TEV). mClover3 is a brighter derivative of mEGFP (PMID: 26879144).

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   Flp-in competent

   Tags

   mClover3-TEV

   Expression

   Mammalian

   Available Since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-Sac-Aβ(M1-42/C18C33)

   Plasmid

   #173759

   Purpose

   Expresses the AβC18C33 peptide.

   Depositor

   James Nowick

   Article

   Zhang et al Biochemistry. 2022 Feb 15;61(4):252-264.

   Insert

   Aβ(M1-42/C18C33) (APP Human)
   

   Expression

   Bacterial

   Mutation

   changed valine 18 to cysteine; changed glycine 33…

   Promoter

   T7

   Available Since

   Aug. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCOTS-pyl-GFP(35TAG)

   Plasmid

   #92047

   Purpose

   This is a S. elongatus (PCC7942) cyanobacterial plasmid that encodes the pylRS orthogonal translation system. In addition it encodes for a GFP reporter with TAG mutation at site 35.

   Depositor

   Lital Alfonta

   Article

   Chemla et al Biochemistry. 2017 Apr 12. doi: 10.1021/

   Inserts

   EGFP
   Pyrrolysyl tRNA(cua)
   Pyrrolysyl tRNA synthetase (methanosarcina mazei)
   

   Use

   Replicative expression plasmid for cyanobacteria …

   Tags

   Histag

   Mutation

   Tyrosine in position 35 of the GFP was mutated f…

   Promoter

   LeuP (native S. elongatus promoter), PpsbII, and …

   Available Since

   Oct. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. BcLOV4 fusion tools screening plasmid #1

   Plasmid

   #174511

   Purpose

   Cloning plasmid for creating BcLOV4 optogenetic tool fusions. Expresses [BamHI]-BcLOV4-[EcoRI]-GGGSx2-mCherry-[XhoI]-STOP in a pcDNA3.1 backbone.

   Depositor

   Brian Chow

   Article

   Berlew et al ACS Synth Biol. 2022 Jan 21;11(1):515-52

   Insert

   [BamHI]-BcLOV4-[EcoRI]-GGGSx2-mCherry-[XhoI]-STOP
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. p5E-1.7kbfabp6

   Plasmid

   #159087

   Purpose

   p5E with promoter sequence 1.7kb upstream of zebrafish fabp6 gene

   Depositor

   John Rawls

   Article

   Wen et al Sci Adv. 2021 Jul 23;7(30). pii: 7/30/ea

   Insert

   1.7kb fabp6 promoter (fabp6 Zebrafish)
   

   Use

   Promoter fragment 5' entry vector for tol2 c…

   Promoter

   1.7kb fabp6

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCR8-PHF19

   Plasmid

   #68864

   Purpose

   GW entry vector for PHF19

   Depositor

   Adrian Bracken

   Article

   Brien et al Nat Struct Mol Biol. 2012 Dec;19(12):127

   Insert

   PHF19 (PHF19 Human)
   

   Use

   Gateway entry vector

   Promoter

   none

   Available Since

   Sept. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pYPQ230-RR

    Plasmid

    #108860

    Purpose

    LbCpf1 Gateway entry plasmid with G548R and K611R mutations

    Depositor

    Yiping Qi

    Article

    Zhong et al Mol Plant. 2018 Jul 2;11(7):999-1002. do

    Insert

    LbCpf1-RR (LbCpf1 with G548R and K611R mutations)
    

    Use

    CRISPR; Gateway compatible cpf1 entry clone

    Tags

    NLS

    Expression

    Plant

    Mutation

    G548R and K611R, Cpf1 is rice codon optimized

    Available Since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKK-TEV-mClover3

    Plasmid

    #105795

    Purpose

    Expression of your protein of interest in fusion with green fluorescent protein at the C-terminus (cleavable by TEV). mClover3 is a brighter derivative of mEGFP (PMID: 26879144).

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    TEV-mClover3

    Expression

    Mammalian

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. p5E-MCS-c-Fos-b-globin (JDW 1237)

    Plasmid

    #229829

    Purpose

    A gateway compatible 5' entry clone containing an MCS upstream of the murine c-fos minimal promotor and b-globin intron.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    c-fos minimal promoter and b-globin intron
    

    Use

    Gateway entry clone

    Expression

    Mammalian

    Available Since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pENTR1A mKate2-hu_ncOGT WT

    Plasmid

    #154282

    Purpose

    Entry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; WT)

    Depositor

    Suzanne Walker

    Article

    Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Use

    Gateway entry vector

    Tags

    2x FLAG and mKate2

    Mutation

    WT OGT

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSF4 TetCMV intron Renilla-STOP 24xPP7 1xfirefly-siRNA xrRNA12 24xMS2 SV40 CTE polyA

    Plasmid

    #104095

    Purpose

    Expresses TREAT-siRNA reporter in mammalian cells

    Depositor

    Jeffrey Chao

    Article

    Horvathova et al Mol Cell. 2017 Nov 2;68(3):615-625.e9. d

    Insert

    Renilla luciferase
    

    Tags

    24xMS2 stem loops in 3'UTR, 24xPP7 stem loop…

    Expression

    Mammalian

    Available Since

    Jan. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKK-TEV-mVenus

    Plasmid

    #105791

    Purpose

    Expression of your protein of interest in fusion with yellow fluorescent protein at the C-terminus (cleavable by TEV). Useful for FRET experiments (PMID: 11753368, 17040988, 22264545).

    Depositor

    Andrzej Dziembowski

    Article

    Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

    Type

    Empty backbone

    Use

    Flp-in competent

    Tags

    TEV-mVenus

    Expression

    Mammalian

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pATZ-BFP-HA-GFP11

    Plasmid

    #177721

    Purpose

    ER protein alpha-1-antitrypsin containing E342K mutation (ATZ mutant) fused to blue fluorescence protein (BFP) tag protein, an HA tag and the eleventh β-strand of GFP

    Depositor

    Erik Böttger

    Article

    Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

    Insert

    alpha-1-antitrypsin (SERPINA1 Human)
    

    Tags

    GFP11, HA, and TagBFP

    Expression

    Bacterial and Mammalian

    Mutation

    E342K (ATZ mutant)

    Available Since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSF4 TetCMV intron Renilla STOP 12xPP7 1xfirefly-siRNA xrRNA12 24xMS2 SV40 CTE polyA

    Plasmid

    #104094

    Purpose

    Expresses TREAT reporter in mammalian cells

    Depositor

    Jeffrey Chao

    Article

    Horvathova et al Mol Cell. 2017 Nov 2;68(3):615-625.e9. d

    Insert

    Renilla luciferase
    

    Tags

    12xPP7 stem loops in 3'UTR, 24xMS2 stem loop…

    Expression

    Mammalian

    Promoter

    TetCMV

    Available Since

    Jan. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. RIP7-RLuc-YFP-pBS

    Plasmid

    #72482

    Purpose

    Expresses a renilla luciferase-YFP fusion protein under control of the rat insulin 2 gene promoter (RIP7) suitable for transfection or generation of transgenic animals

    Depositor

    Vincent Poitout

    Article

    Ghislain et al Endocrinology. 2015 Dec 15:en20151550.

    Inserts

    rat ins2 gene promoter (Ins2 Rat)
    luciferase
    eYFP
    

    Use

    Luciferase

    Tags

    eYFP and renilla luciferase

    Expression

    Mammalian

    Mutation

    eYFP varient

    Available Since

    March 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pT7-G-LbCas12a crRNA-BsaI cassette (MSP3495)

    Plasmid

    #114068

    Purpose

    T7 promoter crRNA entry vector used for in vitro transcription of LbCas12a guides

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    entry vector for LbCas12a crRNAs
    

    Use

    In vitro transcription

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSF4 TetCMV intron Renilla STOP 12xPP7 TNFα-ARE xrRNA12 24xMS2 SV40 CTE polyA

    Plasmid

    #104096

    Purpose

    Expresses TREAT-ARE reporter in mammalian cells

    Depositor

    Jeffrey Chao

    Article

    Horvathova et al Mol Cell. 2017 Nov 2;68(3):615-625.e9. d

    Insert

    Renilla luciferase
    

    Tags

    12xPP7 stem loops in 3'UTR, 24xMS2 stem loop…

    Expression

    Mammalian

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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