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pT7T3D-PacI-Tmeff2

Plasmid

#99728

Purpose

to make in situ RNA probes for Tmeff2

Depositor

Mengsheng Qiu

Article

Huang et al Sci Rep. 2017 Mar 23;7(1):337. doi: 10.1

Insert

Tmeff2 (Tmeff2 Mouse)

Use

In situ probe

Available Since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pAP1071-2

Plasmid

#99502

Purpose

3XFlag::meGFP with 2 introns::Ollas::H2B::tbb-2 3utr::linker::TEV

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

3XFlag::meGFP with 2 introns::Ollas::H2B::tbb-2 3’utr::linker::TEV

Expression

Bacterial

Available Since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

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GAL-MAGE-2-429

Plasmid

#89126

Purpose

mammalian expression of full-length human MAGE-A11 with N-terminal GAL4 DNA binding domain

Depositor

Elizabeth Wilson

Article

Bai et al Mol Cell Biol. 2005 Feb;25(4):1238-57.

Insert

MAGE-A11 (MAGEA11 Human)

Tags

GAL4 DBD

Expression

Mammalian

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only
VP-MAGE-2-429

Plasmid

#89125

Purpose

mammalian expression of full-length human MAGE-A11 with N-terminal VP16 activation domain

Depositor

Elizabeth Wilson

Article

Bai et al Mol Cell Biol. 2005 Feb;25(4):1238-57.

Insert

MAGE-A11 (MAGEA11 Human)

Tags

VP16

Expression

Mammalian

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only
pEN_TTmiRc2 3XFLAG-EGFP-NLS

Plasmid

#87778

Purpose

Used as a donor vector containing N-term 3xFLAG to clone into pSLIK-Hygro

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

eGFP

Tags

3xFLAG and NLS

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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pEN_TTmiRc2 3XFLAG-MKK7-EE-NLS

Plasmid

#87777

Purpose

Used as a donor vector containing N-term 3xFLAG to clone into pSLIK-Hygro

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

MKK7-EE

Tags

3xFLAG and NLS

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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mCBFKOgRNA5

Plasmid

#84366

Purpose

Cas9-gRNA plasmid for mouse Cbfa2t2 Knockout

Depositor

Danny Reinberg

Article

Tu et al Nature. 2016 Jun 8;534(7607):387-90. doi

Insert

Cbfa2t2-gRNA5

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only
mCBFKOgRNA2

Plasmid

#84365

Purpose

Cas9-gRNA plasmid for mouse Cbfa2t2 Knockout

Depositor

Danny Reinberg

Article

Tu et al Nature. 2016 Jun 8;534(7607):387-90. doi

Insert

Cbfa2t2-gRNA2

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only
pHU6_Ch5_155183064-gRNA-for

Plasmid

#81214

Purpose

pHu6-gRNA-NT1_SpecR backbone, gRNA for targeting the 3' region of pCALNL_Ch5 reporter

Depositor

David Liu

Article

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

hU6 expression of gRNA

Expression

Mammalian

Promoter

U6

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pDZ584 pKAN 1x-mCherry

Plasmid

#73172

Purpose

Floxed Kanamycin (G418) selection maker; 3' tagging vector used to create C-terminal fusion proteins with 1x-mCherry

Depositor

Daniel Zenklusen

Article

Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

Insert

1x-mCherry

Use

Unspecified

Available Since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

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His10-PS-SNAPf-9R (XSB525)

Plasmid

#78513

Purpose

Construct for expressing His10 - SNAPf -9R in E. Coli

Depositor

Ron Vale

Article

Su et al Science. 2016 Apr 29;352(6285):595-9. do

Insert

SNAPf

Tags

His10 followed by a prescission protease claevage…

Expression

Bacterial

Mutation

9R added to the C-terminal of SNAPf

Available Since

June 6, 2016

Availability

Academic Institutions and Nonprofits only

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pRS415-Cox15-13xMyc

Plasmid

#61721

Purpose

yeast Cox15 heme A synthase with C-terminal 13xMyc tag in low-copy yeast expression vector

Depositor

Oleh Khalimonchuk

Article

Swenson et al J Biol Chem. 2016 Mar 3. pii: jbc.M115.7

Insert

Cox15 (COX15 Budding Yeast)

Tags

13xMyc

Expression

Yeast

Available Since

Dec. 22, 2015

Availability

Academic Institutions and Nonprofits only
pCVD518

Plasmid

#66158

Purpose

Six copies of the 415 bp PstI fragment of pCVD512, carrying the tdh1 gene probe from Vibrio parahaemolyticus WP1, for generation of probe

Depositor

James Kaper

Article

Nishibuchi et al Infect Immun. 1985 Sep;49(3):481-6.

Insert

tdh1

Use

Cloning vector

Promoter

Unknown

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only
pCVD402

Plasmid

#66148

Purpose

to make a probe to detect Heat stable enterotoxin gene (human)

Depositor

James Kaper

Article

Nishibuchi et al J Clin Microbiol. 1988 Apr;26(4):784-6.

Insert

STh

Use

Cloning vector

Promoter

none

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only
pCVD404

Plasmid

#66149

Purpose

to make a probe to detect Heat stable enterotoxin gene (porcine)

Depositor

James Kaper

Article

Nishibuchi et al J Clin Microbiol. 1988 Apr;26(4):784-6.

Insert

STp

Use

Cloning vector

Promoter

none

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only
pLL3.7 Alk.2 shRNA

Plasmid

#59297

Purpose

shRNA to mouse Alk

Depositor

Ulrike Heberlein

Article

Lasek et al J Neurosci. 2011 Oct 5;31(40):14134-41.

Insert

Alk.2 shRNA

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Promoter

mouse U6

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only
pGEX mPtch-Cterm

Plasmid

#58704

Purpose

used for anti-mouse Patched antibody production (for injection into animals)

Depositor

Matthew Scott

Article

Rohatgi et al Science. 2007 Jul 20;317(5836):372-6.

Insert

Ptch (Ptch1 Mouse)

Tags

GST

Expression

Bacterial

Mutation

contains only Cterminus (aa ~1238-1413)

Promoter

tac

Available Since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only
PCx35-pcDNA-S110A

Plasmid

#42917

Depositor

John O'Brien

Article

Ouyang et al Brain Res Mol Brain Res. 2005 Apr 27;135

Insert

Connexin 35

Expression

Mammalian

Mutation

Ser110 to Ala

Promoter

CMV

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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PCx35CT-S298ter

Plasmid

#42913

Depositor

John O'Brien

Article

Ouyang et al Brain Res Mol Brain Res. 2005 Apr 27;135

Insert

Connexin 35 C-terminal domain

Tags

6x His, GST, and S-tag

Expression

Bacterial

Mutation

Ser298 stop codon

Promoter

T7

Available Since

March 26, 2013

Availability

Academic Institutions and Nonprofits only

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PCx35-pcDNA-S298ter

Plasmid

#42925

Depositor

John O'Brien

Article

Ouyang et al Brain Res Mol Brain Res. 2005 Apr 27;135

Insert

Connexin 35

Expression

Mammalian

Mutation

Ser298 to stop codon

Promoter

CMV

Available Since

March 26, 2013

Availability

Academic Institutions and Nonprofits only

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PCx35CT-S276A

Plasmid

#42912

Depositor

John O'Brien

Article

Ouyang et al Brain Res Mol Brain Res. 2005 Apr 27;135

Insert

Connexin 35 C-terminal domain

Tags

6x His, GST, and S-tag

Expression

Bacterial

Mutation

Ser276 to Ala

Promoter

T7

Available Since

March 26, 2013

Availability

Academic Institutions and Nonprofits only

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sugarless cDNA

Plasmid

#42063

Depositor

Norbert Perrimon

Article

Hacker et al Development. 1997 Sep;124(18):3565-73.

Insert

sugarless (sgl Fly)

Available Since

Feb. 21, 2013

Availability

Academic Institutions and Nonprofits only
pFM114

Plasmid

#40160

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-3N+S251A (par-3 Nematode)

Tags

GST

Expression

Bacterial

Mutation

N terminal portion of protein (aa 1-364); S251A

Promoter

tac

Available Since

Oct. 23, 2012

Availability

Academic Institutions and Nonprofits only
pFM116

Plasmid

#40162

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-3C+S950A (par-3 Nematode)

Tags

GST

Expression

Bacterial

Mutation

C terminal portion of protein (aa840-1268); S950A

Promoter

tac

Available Since

Sept. 12, 2012

Availability

Academic Institutions and Nonprofits only
Bcr del PK

Plasmid

#36422

Depositor

John Groffen, Nora Heisterkamp

Article

Yi et al J Biol Chem. 2009 Dec 18;284(51):35645-5

Insert

BCR (BCR Human)

Expression

Mammalian

Mutation

deletion of residues 40-447

Available Since

Aug. 27, 2012

Availability

Academic Institutions and Nonprofits only
pCS2-F1-GGA-F2-GTG-F3-TTG

Plasmid

#30701

Depositor

Nathan Lawson, Scot Wolfe

Article

Zhu et al Development. 2011 Oct;138(20):4555-64.

Insert

F1 GGA, F2 GTG, F3 TTG

Use

Mrna production

Tags

FLAG

Available Since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
pCS2-F1-GGC

Plasmid

#30702

Depositor

Nathan Lawson, Scot Wolfe

Article

Zhu et al Development. 2011 Oct;138(20):4555-64.

Insert

F1 GGC

Use

Mrna production

Tags

FLAG

Available Since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
pCS2-F1-GGT-F3-CAG

Plasmid

#30704

Depositor

Nathan Lawson, Scot Wolfe

Article

Zhu et al Development. 2011 Oct;138(20):4555-64.

Insert

F1 GGT, F3 CAG

Use

Mrna production

Tags

FLAG

Available Since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
pCS2-F1-GTG-F2-GGT-F3-GAA

Plasmid

#30707

Depositor

Nathan Lawson, Scot Wolfe

Article

Zhu et al Development. 2011 Oct;138(20):4555-64.

Insert

F1 GTG, F2 GGT, F3 GAA

Use

Mrna production

Tags

HA

Available Since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
pCS2_F1-GTT

Plasmid

#30708

Depositor

Nathan Lawson, Scot Wolfe

Article

Zhu et al Development. 2011 Oct;138(20):4555-64.

Insert

F1 GTT

Use

Mrna production

Tags

FLAG

Available Since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only