We narrowed to 4,339 results for: 187

1. pXD70EN25-Pct5-(–)-Eddh(Gp3C)

   Plasmid

   #233877

   Purpose

   Expresses Gordonibacter pamelaeae 3C (–)-Eddh in Eggerthella lenta DSM 2243 with a cumate inducible promoter

   Depositor

   Emily Balskus

   Article

   Dong et al J Am Chem Soc. 2025 Mar 5;147(9):7231-72

   Insert

   (–)-Eddh
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. MDH1_KW

   Plasmid

   #224145

   Purpose

   IPTG-inducible expression of C. elegans protein MDH-1 with a chitin tag

   Depositor

   Katherine Walstrom

   Article

   Thomas et al Biochim Biophys Acta Proteins Proteom. 2

   Insert

   MDH-1 (F46E10.10 Nematode)
   

   Use

   Tags

   Ala-intein-CBD

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. FLAG-Bmp7

   Plasmid

   #201909

   Purpose

   in vitro transcription of RNA encoding FLAG-tagged Bmp7a

   Depositor

   Mary Mullins

   Article

   Little et al Nat Cell Biol. 2009 May;11(5):637-43. do

   Insert

   bmp7 (bmp7a Zebrafish)
   

   Use

   Tags

   FLAG tag

   Expression

   Mutation

   Promoter

   SP6

   Available since

   June 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. LDB221

   Plasmid

   #185427

   Purpose

   Bacterial expression of Nup1 C-terminal residues 777-1076 as GST fusion

   Depositor

   Laura Davis, Ken Belanger

   Article

   Belanger et al CBE Life Sci Educ. 2009 Fall;8(3):214-25

   Insert

   NUP1
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Nup1 C-terminal residues 777-1076

   Promoter

   Available since

   July 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. LDB203

   Plasmid

   #185424

   Purpose

   Bacterial expression of N-terminus of NUP1 nucleoporin as GST-Nup1-N fusion

   Depositor

   Laura Davis, Ken Belanger

   Article

   Belanger et al CBE Life Sci Educ. 2009 Fall;8(3):214-25

   Insert

   NUP1
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Nup1 N-terminal domain only

   Promoter

   Available since

   July 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. LDB205

   Plasmid

   #185425

   Purpose

   Bacterial expression of C-terminus of NUP1 nucleoporin as GST-Nup1-C fusion

   Depositor

   Laura Davis, Ken Belanger

   Article

   Belanger et al CBE Life Sci Educ. 2009 Fall;8(3):214-25

   Insert

   NUP1
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Nup1 C-terminal domain only

   Promoter

   Available since

   July 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. KBB101

   Plasmid

   #185064

   Purpose

   Bacterial expression of C-terminus of NUP1 nucleoporin as GST-Nup1-C fusion truncated after the last FXFG repeat

   Depositor

   Ken Belanger

   Article

   Belanger et al CBE Life Sci Educ. 2009 Fall;8(3):214-25

   Insert

   NUP1
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Nup1 C-terminal region, truncated

   Promoter

   Available since

   June 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFOSGE_COF2E10

   Plasmid

   #70929

   Purpose

   Gateway entry clone

   Depositor

   Alexander Stark

   Article

   Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

   Insert

   Elp2 (Elp2 Fly)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pNB762

   Plasmid

   #60150

   Purpose

   Firefly luciferase (Promega) driven by LEU1 promoter.

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   FLuc
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   FLuc has A4V & S504G (no functional effect)

   Promoter

   LEU1pr

   Available since

   March 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNB767

    Plasmid

    #60159

    Purpose

    Firefly luciferase (Promega) driven by SIC1 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    FLuc
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    A4V & S504G (no functional effect)

    Promoter

    SIC1pr

    Available since

    March 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. ZJOM151

    Plasmid

    #133643

    Purpose

    Integration of COX4-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast mitochondria.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-COX4-GFP (COX4 Budding Yeast)
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. LSM0254 (PB)EF1a-ADAR2-T2A-PuroR-P2A-BFP

    Plasmid

    #233445

    Purpose

    PiggyBac donor plasmid to overexpress a full length ADAR2

    Depositor

    Xiaojing Gao

    Article

    Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

    Insert

    ADAR2-T2A-PuroR-P2A-BFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    EF1a

    Available since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRK5M-ADAM10

    Plasmid

    #31717

    Depositor

    Rik Derynck

    Article

    Liu et al Mol Cell. 2009 Jul 10. 35(1):26-36.

    Insert

    ADAM10 (ADAM10 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-11b_PcrA_M6_H93A

    Plasmid

    #194156

    Purpose

    Engineered PcrA_M6 helicase to support isothermal amplification method SHARP

    Depositor

    Taekjip Ha

    Article

    Gavrilov et al Nat Commun. 2022 Oct 23;13(1):6312. doi:

    Insert

    Engineered helicase PcrA_M6
    

    Use

    Tags

    6x His tag and FLAG tag

    Expression

    Bacterial

    Mutation

    H93A, C96A, N187C, C247A, L384V, L409C

    Promoter

    Available since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCS4333 yeTadA1.0-T7 RNAP

    Plasmid

    #137735

    Purpose

    pCS4333 CEN/ARS vector, PGAL1-yeTadA1.0-100AA linker-T7 RNAP-TCYC1, TRP1 selectable marker

    Depositor

    Christina Smolke

    Article

    Cravens et al Nat Commun. 2021 Mar 11;12(1):1579. doi:

    Insert

    yeTadA1.0-T7 RNAP
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    E366G (See depositor comment below)

    Promoter

    Available since

    Jan. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti6.3-enCjCas9-BlastR

    Plasmid

    #220978

    Purpose

    Expresses human codon-optimized enCjCas9

    Depositor

    Hyongbum Kim

    Article

    Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

    Insert

    enCjCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLKO-shBRCA1 #1

    Plasmid

    #44594

    Depositor

    Eros Lazzerini Denchi

    Article

    Okamoto et al Nature. 2013 Feb 6. doi: 10.1038/nature1

    Insert

    BRCA1 (Brca1 Mouse)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pLKO-shBRCA1 #2

    Plasmid

    #44595

    Depositor

    Eros Lazzerini Denchi

    Article

    Okamoto et al Nature. 2013 Feb 6. doi: 10.1038/nature1

    Insert

    BRCA1 (Brca1 Mouse)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pLenti6.3-sRGN3.1-BlastR

    Plasmid

    #220964

    Purpose

    Expresses human codon-optimized sRGN3.1

    Depositor

    Hyongbum Kim

    Article

    Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

    Insert

    sRGN3.1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCali2

    Plasmid

    #86119

    Purpose

    Multiplex temperature-gated expression of mWasabi and mCherry reporters

    Depositor

    Mikhail Shapiro

    Article

    Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

    Inserts

    TlpA36
    mWasabi
    TcI
    mCherry
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    A67T and D341A, P60L, D135V, K187R, K202I, L208Q

    Promoter

    pR/pL, pR/pL, pLacI, pTlpA, and pTlpA, pLacI

    Available since

    Feb. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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