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miniTol2 x EF1a_EKAREN4 + PGK-blast

Plasmid

#167825

Purpose

Optimized EKAREV FRET biosensor sensor for ERK

Depositor

Hugo Snippert

Article

Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

Insert

EKAREN4

Tags

nls localization motif

Expression

Mammalian

Mutation

K424P, K426W

Promoter

EF1a

Available Since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5 rtTA BirA eIF4G

Plasmid

#158789

Purpose

pcDNA5 based transfection plasmid for the exogenous expression of N-terminal BirA-Flag-tagged eIF4G1 lacking the microexon for generation of N2A FlpIn cell lines for BioID

Depositor

Benjamin Blencowe

Article

Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1

Insert

eIF4G1

Tags

3xFlag and BirA*

Expression

Mammalian

Promoter

miniCMV

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pLV-hU6-FOXP2_sgRNA1-hUbC-dCas9-KRAB-T2a-GFP

Plasmid

#231022

Purpose

All-in-one lentiviral vector (Gersbach lab design) for CRISPRi targeting FOXP2

Depositor

Tomasz Nowakowski

Article

Shin et al Cell Stem Cell. 2024 Mar 7;31(3):421-432

Insert

FOXP2 sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-hU6-FOXP2_sgRNA2-hUbC-dCas9-KRAB-T2a-GFP

Plasmid

#231023

Purpose

All-in-one lentiviral vector (Gersbach lab design) for CRISPRi targeting FOXP2

Depositor

Tomasz Nowakowski

Article

Shin et al Cell Stem Cell. 2024 Mar 7;31(3):421-432

Insert

FOXP2 sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-hU6-ROBO2_sgRNA-hUbC-dCas9-KRAB-T2a-GFP

Plasmid

#231024

Purpose

All-in-one lentiviral vector (Gersbach lab design) for CRISPRi targeting ROBO2

Depositor

Tomasz Nowakowski

Article

Shin et al Cell Stem Cell. 2024 Mar 7;31(3):421-432

Insert

ROBO2 sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-hU6-NTC_sgRNA-hUbC-dCas9-KRAB-T2a-GFP

Plasmid

#231025

Purpose

All-in-one lentiviral vector (Gersbach lab design) for CRISPRi with non-targeting control sgRNA

Depositor

Tomasz Nowakowski

Article

Shin et al Cell Stem Cell. 2024 Mar 7;31(3):421-432

Insert

Non-targeting control sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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PB CRIV Gc Spike H6

Plasmid

#240163

Purpose

Plasmid for making PiggyBac stable cell line expressing Cristoli virus (CRIV) glycoprotein Gc spike with a C-terminal His6 tag

Depositor

Stephen Graham

Article

Merchant et al EMBO Mol Med. 2025 Aug 11. doi: 10.1038/

Insert

CRIV Gc spike

Use

Piggybac

Tags

6xHis

Expression

Mammalian

Mutation

contains residues 478-906 only

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pMWCAVI_H6BAP-OROV_N

Plasmid

#240162

Purpose

Plasmid encoding Oropouche virus (OROV) nucleoprotein (N) with an N-terminal His6 and biotin acceptor peptide (BAP, a.k.a. AviTag) for expression in E. coli

Depositor

Stephen Graham

Article

Merchant et al EMBO Mol Med. 2025 Aug 11. doi: 10.1038/

Insert

Nucleoprotein

Tags

6xHis, Biotin acceptor peptide (BAP, a.k.a. AviTa…

Expression

Bacterial

Available Since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-GSDMD_H270A

Plasmid

#224701

Purpose

Expression of mutated Gasdermin D_H270A (GSDMD_H270A) in transfected mammalian cell lines

Depositor

Christopher Overall

Article

Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

Insert

GSDMD_H270A (GSDMD Human)

Tags

FLAG

Expression

Mammalian

Mutation

H270A

Promoter

CMV

Available Since

July 2, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1-GSDMD_Q29A

Plasmid

#224702

Purpose

Expression of mutated Gasdermin D_Q29A (GSDMD_Q29A) in transfected mammalian cell lines

Depositor

Christopher Overall

Article

Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

Insert

GSDMD_Q29A (GSDMD Human)

Tags

FLAG

Expression

Mammalian

Mutation

Q29A

Promoter

CMV

Available Since

July 2, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1-GSDMD_Q29A_H270A

Plasmid

#224703

Purpose

Expression of mutant Gasdermin D_Q29A_H270A (GSDMD_Q29A_H270A) in transfected mammalian cell lines

Depositor

Christopher Overall

Article

Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

Insert

GSDMD_Q29A_H270A (GSDMD Human)

Tags

FLAG

Expression

Mammalian

Mutation

Q29A and H270A

Promoter

CMV

Available Since

July 2, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1-GSDMD_D275A

Plasmid

#224704

Purpose

Expression of mutated Gasdermin D_D275A (GSDMD_D275A) in transfected mammalian cell lines

Depositor

Christopher Overall

Article

Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

Insert

GSDMD_D275A (GSDMD Human)

Tags

FLAG

Expression

Mammalian

Mutation

D275A

Promoter

CVM

Available Since

July 2, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1-GSDMD_H270A_D275A

Plasmid

#224705

Purpose

Expression of mutated Gasdermin D_H270A_D275A (GSDMD_D275A) in transfected mammalian cell lines

Depositor

Christopher Overall

Article

Grin et al Cell Rep. 2024 Dec 24;43(12):115080. doi

Insert

GSDMD_Q29A_H270A (GSDMD Human)

Tags

FLAG

Expression

Mammalian

Mutation

H270A and D275A

Promoter

CMV

Available Since

July 2, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pMRX-IP-EGFP-PA-BAX(ER, G108V)

Plasmid

#233344

Purpose

Stable expression of EGFP-LOV2(N538E)-BAX(G108V)-Cyb5a in mammalian cells. The G108V mutation suppresses pore-forming activity of BAX.

Depositor

Noboru Mizushima

Article

Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.

Inserts

LOV2 domain of phototropin1
BAX (BAX Human)
Cyb5a (Cyb5a Rat)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

BAX(3-171), N-terminus and C-terminus are deleted…

Available Since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-MCP-Lbr-V5-mCherry

Plasmid

#235093

Purpose

Lbr mCherry fusion protein with MCP domain

Depositor

Mitchell Guttman

Article

Bhat et al Nature. 2024 May;629(8014):1165-1173. do

Insert

pCAG-MCP-Lbr-V5-mCherry (LBR Human)

Expression

Mammalian

Promoter

CAG

Available Since

May 7, 2025

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-EGFP-PA-BAX(lyso, G108V)

Plasmid

#233345

Purpose

Stable expression of EGFP-LOV2(G528A, N538E)-BAX-TMEM106B in mammalian cells. The G108V mutation suppresses pore-forming activity of BAX.

Depositor

Noboru Mizushima

Article

Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.

Inserts

LOV2 domain of phototropin1
BAX (BAX Human)
TMEM106B (TMEM106B Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

BAX(3-171), N-terminus and C-terminus are deleted…

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pBIG1a_StrepII-Hook2_FTS_FHIP2A

Plasmid

#222302

Purpose

Co-expresses full-length human FHF (FTS, StrepII-HOOK2 and FHIP2A) in a pBIG1a vector

Depositor

Andrew Carter

Article

Abid Ali et al Nat Struct Mol Biol. 2025 Apr;32(4):756-

Inserts

HOOK2 (HOOK2 Human)
FTS (AKTIP Human)
FHIP2A (FHIP2A Human)

Tags

StrepII-Psc

Expression

Insect

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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Lenti-N1303K-ngRNA+14_EF1a-puroR

Plasmid

#207359

Purpose

Lentiviral transfer plasmid encoding hU6-driven expression of a ngRNA for correction of N1303K-CFTR via prime editing and EF1a-driven puromycin resistance gene.

Depositor

Marianne Carlon

Article

Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

Insert

N1303K-CFTR +14 ngRNA

Use

Lentiviral

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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Lenti-L227R-ngRNA+15_EF1a-puroR

Plasmid

#207360

Purpose

Lentiviral transfer plasmid encoding hU6-driven expression of a ngRNA for correction of L227R-CFTR via prime editing and EF1a-driven puromycin resistance gene.

Depositor

Marianne Carlon

Article

Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

Insert

L227R-CFTR +15 ngRNA

Use

Lentiviral

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3_ATP10B-G671R-N865K

Plasmid

#203699

Purpose

expresses ATP10B with the pathogenic mutations G671R and N865K

Depositor

Peter Vangheluwe

Article

Martin et al Acta Neuropathol. 2020 Jun;139(6):1001-1

Insert

ATP10B (ATP10B Human)

Expression

Mammalian

Mutation

G671R, N865K

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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