We narrowed to 8,593 results for: Set

1. Anti-Pancortin [K96/7R]

   Plasmid

   #182098

   Purpose

   Mammalian Expression Plasmid of anti-Pancortin (Human). Derived from hybridoma K96/7.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   anti-Pancortin (Homo sapiens) recombinant mouse monoclonal antibody (OLFM1 Mouse)
   

   Expression

   Mammalian

   Promoter

   Dual CMV

   Available Since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Rufy3 scFv [N483/126]

   Plasmid

   #182089

   Purpose

   Mammalian Expression of Rufy3 scFv. Derived from hybridoma N483/126.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   recombinant mouse scFv targeting Rufy3 (Mus musculus) (Rufy3 Mouse)
   

   Tags

   Sortase, His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Anti-Synapsin-3 [L125/121R]

   Plasmid

   #177471

   Purpose

   Mammalian Expression Plasmid of anti-Synapsin-3 (Human). Derived from hybridoma L125/121.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   anti-Synapsin-3 (Homo sapiens) recombinant mouse monoclonal antibody (SYN3 Mouse)
   

   Expression

   Mammalian

   Promoter

   Dual CMV

   Available Since

   Jan. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Anti-Pan-MAGUK [K28/86R]

   Plasmid

   #177440

   Purpose

   Mammalian Expression Plasmid of anti-Pan-MAGUK (Human). Derived from hybridoma K28/86.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   anti-Pan-MAGUK (Homo sapiens) recombinant mouse monoclonal antibody
   

   Expression

   Mammalian

   Promoter

   Dual CMV

   Available Since

   Nov. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGG442

   Plasmid

   #165616

   Purpose

   Vector for expression of SpCas9-Zif268 in yeast after assembly with PID fragments: TEF1p-SpCas9::KanR-1xNLS-3xHA-1xNLS-Zif268-1xNLS-CYC1t (KanR cassette in place of the PID)

   Depositor

   Marcus Noyes

   Article

   Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

   Insert

   TEF1p-SpCas9::KanR(in place of PID)-1xNLS-3xHA-1xNLS-Zif268-1xNLS-CYC1t
   

   Use

   CRISPR

   Tags

   3x HA, SV40 NLS, Zif268, and c-Myc-like NLS

   Expression

   Yeast

   Mutation

   Corrected homology relative to wild type SpCas9 (…

   Promoter

   TEF1

   Available Since

   April 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJEP324-pAAV-FullH1TO-SaCa9sgRNAi(emx1sg1)-CMV-TetR-2A-EGFP-KASH-WPRE-shortPA

   Plasmid

   #113701

   Purpose

   Dox-inducible H1 driven SaCas9 gRNA expression cassette with a gRNA targeting EMX1. Followed by an EFS driven GFP-KASH in a separate reading frame.

   Depositor

   Jonathan Ploski

   Article

   Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

   Inserts

   SaCas9 gRNA Cassete
   GFP
   

   Use

   AAV and CRISPR

   Tags

   KASH

   Available Since

   Feb. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJEP322-pAAV-FullU6TO-SaCa9sgRNAi(emx1-sg1)-CMV-TetR-2A-EGFP-KASH-WPRE-shortPA

   Plasmid

   #113699

   Purpose

   Dox-inducible U6 driven SaCas9 gRNA expression cassette with a gRNA targeting EMX1. Followed by an EFS driven GFP-KASH in a separate reading frame.

   Depositor

   Jonathan Ploski

   Article

   Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

   Inserts

   SaCas9 gRNA Cassete
   GFP
   

   Use

   AAV and CRISPR

   Tags

   KASH

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3.1 Flag YFP hNKCC1 intracellular-cysteineless (NT855)

   Plasmid

   #49080

   Purpose

   Expresses human NKCC1 mutant lacking cysteine residues within intracellular loops and with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

   Depositor

   Biff Forbush

   Article

   Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

   Insert

   human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
   

   Tags

   3x Flag and mVenus

   Expression

   Mammalian

   Mutation

   C791S, C820S, C1052S in hNKCC1 in NT17

   Promoter

   CMV

   Available Since

   Nov. 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1 Flag YFP hNKCC1 ECL4 deletion (NT865)

   Plasmid

   #49070

   Purpose

   Expresses human NKCC1 truncation mutant lacking extracellular loop #4 (aa562-582) and with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

   Depositor

   Biff Forbush

   Article

   Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

   Insert

   human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
   

   Tags

   3x Flag and mVenus

   Expression

   Mammalian

   Mutation

   ECL4 deletion of amino acids 562-582 in hNKCC1 in…

   Promoter

   CMV

   Available Since

   Nov. 19, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1 Flag YFP hNKCC1 cysteineless (NT868)

    Plasmid

    #49079

    Purpose

    Expresses human NKCC1 mutant lacking cysteine residues and with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Tags

    3x Flag and mVenus

    Expression

    Mammalian

    Mutation

    C295A, C543M, C563S,C568S,C577S,C582S, C630S, C72…

    Promoter

    CMV

    Available Since

    Nov. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.1 Flag YFP hNKCC1 TM-cysteineless (NT859)

    Plasmid

    #49078

    Purpose

    Expresses human NKCC1 mutant lacking cysteine residues within transmembrane domains and with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic,containing convenient restriction sites

    Depositor

    Biff Forbush

    Article

    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Tags

    3x Flag and mVenus

    Expression

    Mammalian

    Mutation

    C295A, C543M, C630S, C723S, C724V in hNKCC1 in NT…

    Promoter

    CMV

    Available Since

    Nov. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEJS1099: Dual-sgRNA.Design 4

    Plasmid

    #159537

    Purpose

    Delivery of dual sgRNA cassettes and Nme2Cas9 in a single AAV vector

    Depositor

    Erik Sontheimer

    Article

    Ibraheim et al Nat Commun. 2021 Nov 1;12(1):6267. doi:

    Insert

    Nme2Cas9 with two guide RNA cassettes
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    NLS

    Expression

    Mammalian

    Promoter

    U1a

    Available Since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W

    Plasmid

    #67974

    Purpose

    CRISPR gRNA expression vector with an improved scaffold and puro/BFP markers

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKpuro2ABFP cassette, WPRE
    

    Use

    Lentiviral

    Mutation

    Deleted BbsI site within WPRE

    Promoter

    human U6 promoter, mouse PGK promoter

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. FLAG-JAK1

    Plasmid

    #174572

    Purpose

    Human FLAG-tagged JAK1 expression (N-term. tag) (CMV prom.)

    Depositor

    Mathew Garnett

    Article

    Coelho et al Cancer Cell. 2023 Feb 13;41(2):288-303.e

    Insert

    JAK1 (JAK1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pUCmini-iCAP-AAV.CAP-B10

    Plasmid

    #175004

    Purpose

    non-standard AAV2 rep-AAV.CAP-B10 cap plasmid with AAV cap expression controlled by a tTA-TRE amplifcation system

    Depositor

    Viviana Gradinaru

    Article

    Goertsen et al Nat Neurosci. 2022 Jan;25(1):106-115. do

    Insert

    Synthetic construct isolate AAV.CAP-B10 VP1 gene
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    7 amino acid substitution between VP1 452 and VP1…

    Promoter

    p41

    Available Since

    Nov. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pKLV2-U6gRNA5(gBFP)-PGKmCherry2ABFP-W

    Plasmid

    #67986

    Purpose

    Cas9 activity reporter with mCherry and BFP

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Inserts

    U6gRNA cassette, PGKmCherryABFP cassette, WPRE
    Guide RNA targeting modified BFP
    

    Use

    CRISPR and Lentiviral

    Mutation

    Deleted BbsI site within WPRE, modified BFP

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. AICSDP-49: MYL2-mEGFP

    Plasmid

    #114414

    Purpose

    Homology arms and linker-mEGFP sequence for C-terminus tagging of human MYL2, via Cas9-excisable CAGGS-mCherry selection cassette

    Depositor

    Allen Institute for Cell Science

    Article

    Roberts et al Stem Cell Reports. 2019 May 14;12(5):114

    Insert

    MYL2 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (MYL2 Human)
    

    Use

    CRISPR; Donor template

    Tags

    linker-mEGFP

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Available Since

    Sept. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Anti-Nav1.2 Na+ channel [K69/33R]

    Plasmid

    #199396

    Purpose

    Mammalian expression plasmid of Anti-Nav1.2 Na+ channel [K69/33R] (Rat). Derived from hybridoma K69/33.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    Anti-Nav1.2 Na+ channel (Rat) recombinant mouse monoclonal antibody (Scn2a Mouse)
    

    Expression

    Mammalian

    Promoter

    Dual CMV

    Available Since

    Sept. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Anti-Nav1.2 Na+ channel [K69/3R]

    Plasmid

    #206520

    Purpose

    Mammalian Expression Plasmid of anti-Nav1.2 Na+ channel (Rat). Derived from hybridoma K69/3.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-Nav1.2 Na+ channel (Rattus norvegicus) recombinant Mouse monoclonal antibody (Scn2a Mouse)
    

    Expression

    Mammalian

    Promoter

    Dual CMV

    Available Since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUCmini-iCAP-AAV.CAP-B22

    Plasmid

    #175005

    Purpose

    non-standard AAV2 rep-AAV.CAP-B22 cap plasmid with AAV cap expression controlled by a tTA-TRE amplifcation system

    Depositor

    Viviana Gradinaru

    Article

    Goertsen et al Nat Neurosci. 2022 Jan;25(1):106-115. do

    Insert

    Synthetic construct isolate AAV.CAP-B22 VP1 gene
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    7 amino acid substitution between VP1 452 and VP1…

    Promoter

    p41

    Available Since

    Nov. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge