We narrowed to 4,634 results for: HRE

1. pME-mTagBFP

   Plasmid

   #75148

   Purpose

   TagBFP (blue) fluorophore for use with 5’ promoters.

   Depositor

   Nicholas Cole

   Article

   Don et al Zebrafish. 2016 Sep 15.

   Insert

   mTagBFP
   

   Use

   Middle entry vector for multisite gateway three f…

   Tags

   Expression

   Mutation

   Promoter

   n/a

   Available Since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. PCT62

   Plasmid

   #170391

   Purpose

   expresses three sgRNA cassettes

   Depositor

   Jonathan Weissman

   Article

   Quinn et al Science. 2021 Feb 26;371(6532). pii: sci

   Insert

   PCT62-targetsite
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGK401red-­rtetTAK8N, L131L

   Plasmid

   #165968

   Purpose

   Expresses rTetTA (K8N, L131L) mutant

   Depositor

   Jun Ishii

   Article

   Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

   Insert

   tetR
   

   Use

   Synthetic Biology

   Tags

   three copies of VP16 (VP48)

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRST11B-AS3_4

   Plasmid

   #102789

   Purpose

   Expresses tryptophanyl tRNA synthetase (ScWRS) and supressor tryptophanyl tRNA

   Depositor

   Andrew Ellington

   Article

   Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

   Inserts

   tryptophanyl tRNA synthetase (WRS1 Budding Yeast)
   suppressor tRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 3, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pGP-FB-orig BA

   Plasmid

   #13420

   Depositor

   Keith Joung

   Article

   Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

   Insert

   orig BA
   

   Use

   Tags

   Gal11P

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 8, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGL3 GATA Luc

   Plasmid

   #85695

   Purpose

   Luciferase reporter containing three repeats of the GATA consensus site

   Depositor

   Licio Collavin, Giannino Del Sal

   Article

   Collavin et al Proc Natl Acad Sci U S A. 2004 Jun 15;10

   Insert

   3xGATA and minimal MT promoter
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   3xGATA and minimal MT promoter

   Available Since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

7. pCH1 - pBait-empty

   Plasmid

   #174663

   Purpose

   arabinose-induced synthesis of an RNA containing a single MS2 hairpin. Used as a three-hybrid control. For cloning purposes, see plasmid #174662.

   Depositor

   Katherine Berry

   Article

   Pandey et al Nucleic Acids Res. 2020 May 7;48(8):4507

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEYFP-N1-HsSmg7_H

   Plasmid

   #146476

   Purpose

   Mammalian Expression of HsSmg7

   Depositor

   Elisa Izaurralde

   Article

   Unterholzner et al Mol Cell. 2004 Nov 19. 16(4):587-96.

   Insert

   HsSmg7 (SMG7 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   one non silent mutation G2560A (V854I) and three …

   Promoter

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAc5.1B-EGFP-DmFMR1_B

   Plasmid

   #145920

   Purpose

   Insect Expression of DmFMR1

   Depositor

   Elisa Izaurralde

   Insert

   DmFMR1 (Fmr1 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Two amino acid mutations (T214I and N501S) and th…

   Promoter

   Available Since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. YFP-Rac2(N17)

    Plasmid

    #11398

    Depositor

    Joel Swanson

    Article

    Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

    Insert

    ras-related C3 botulinum toxin substrate 2 (RAC2 Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Obtained with Threonine 17 changed to Asparagine.

    Promoter

    Available Since

    Feb. 21, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEYFP-N1-HsSmg5_H

    Plasmid

    #146474

    Purpose

    Mammalian Expression of HsSmg5

    Depositor

    Elisa Izaurralde

    Article

    Unterholzner et al Mol Cell. 2004 Nov 19. 16(4):587-96.

    Insert

    HsSmg5 (SMG5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    three non silent mutation A254G (K85R), T980C (L3…

    Promoter

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEG476

    Plasmid

    #40198

    Depositor

    Geraldine Seydoux

    Article

    Griffin et al Cell. 2011 Sep 16;146(6):955-68.

    Insert

    Mex-5 (S404A) (mex-5 Nematode)
    

    Use

    Tags

    Dendra2 PAFP (sequence optimized for worm express…

    Expression

    Worm

    Mutation

    S404A

    Promoter

    pie-1

    Available Since

    Oct. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

13. Venus-Bik-DD-pEGFP-C1

    Plasmid

    #177429

    Purpose

    To express Venus fused to the N-terminus of the Bcl-2 family protein, Bik, phosphomimetic mutant. T33D and S35D mutations made to Addgene #166737.

    Depositor

    David Andrews

    Insert

    Bik, Bcl-2-interacting killer, or Apoptosis inducer NBK (BIK Human)
    

    Use

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Threonine 33 and Serine 35 replaced with aspartic…

    Promoter

    CMV

    Available Since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-pOTC-3H-GFP

    Plasmid

    #205725

    Purpose

    Expression of GFP fused with pOTC-3H, a leader peptide from mitochondrial matrix enzyme ornithine transcarbamylase and a thermostable three helix-bundle

    Depositor

    Randy Schekman

    Article

    Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

    Insert

    pOTC-3H-GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDNA3 10 mut B-Myb

    Plasmid

    #25966

    Depositor

    Rob Lewis

    Article

    Johnson et al J Biol Chem. 1999 Dec 17. 274(51):36741-

    Insert

    B-Myb (Mybl2 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    changed threonines 267, 408, 497, 519, 522 and 52…

    Promoter

    Available Since

    Sept. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

16. pGK415-­rtetTAK8N, L131L

    Plasmid

    #165969

    Purpose

    Expresses rTetTA (K8N, L131L) mutant

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

    Insert

    tetR
    

    Use

    Synthetic Biology

    Tags

    three copies of VP16 (VP48)

    Expression

    Yeast

    Mutation

    Promoter

    pPGK1

    Available Since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. p3PA-Puro-iTk

    Plasmid

    #109067

    Purpose

    inserts three polyA sites to end transcription of a targetted gene and a puromycin cassette for selection

    Depositor

    Eric Lander

    Article

    Engreitz et al Nature. 2016 Nov 17;539(7629):452-455. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available Since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAc5.1B-lambdaN-HA-DmFMR1_B

    Plasmid

    #145940

    Purpose

    Insect Expression of DmFMR1

    Depositor

    Elisa Izaurralde

    Insert

    DmFMR1 (Fmr1 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Two amino acid mutations (N45D and G558R) and thr…

    Promoter

    Available Since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLenti-CaMKIIa-hChR2(C128T)-EYFP-WPRE

    Plasmid

    #20295

    Purpose

    Lentiviral expression of ChR2 (C128T) Step Function Opsin (SFO) fused to EYFP, for bi-stable optogenetic excitation

    Depositor

    Karl Deisseroth

    Article

    Berndt et al Nat Neurosci. 2009 Feb . 12(2):229-34.

    Insert

    channelrhodopsin-2
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    changed Cysteine 128 to Threonine

    Promoter

    Available Since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. pR184

    Plasmid

    #47952

    Purpose

    rtTA positive feedback loop with three tet operators in the GAL1 promoter

    Depositor

    Attila Becskei

    Article

    Hsu et al Nat Commun. 2012 Feb 21;3:682. doi: 10.1

    Insert

    rtTA
    

    Use

    Tet inducible expression

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Gal1

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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