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pnYC-NpM-DmNot1_1468-1719_AF

Plasmid

#148740

Purpose

Bacterial Expression of DmNot1_1468-1719

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

DmNot1_1468-1719 (Not1 Human)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pACT2-μ1B (137-423)

Plasmid

#198172

Purpose

Expression of GAL4 transcriptional activation domain (AD)-AP-1 μ1B (137-423) fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

Insert

AP-1 μ1B (137-423)

Tags

GAL4 transcriptional activation domain (AD) fragm…

Expression

Yeast

Promoter

ADH1

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGBT9-AAGAB

Plasmid

#198332

Purpose

Expression of GAL4 DNA-binding domain (BD)-AAGAB fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

Insert

AAGAB (AAGAB Human)

Tags

GAL4-DNA binding domain (BD) fragment

Expression

Yeast

Promoter

ADH1

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpH-DmCaf40_25-291-Y139DG146W_AF

Plasmid

#148736

Purpose

Bacterial Expression of DmCaf40_25-291-Y139DG146W

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

DmCaf40_25-291-Y139DG146W (Rcd-1 Fly)

Expression

Bacterial

Available Since

Jan. 13, 2023

Availability

Academic Institutions and Nonprofits only

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Chinchilla–insM

Plasmid

#191226

Purpose

Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

Depositor

Susanne Dobler

Article

Mohammadi et al Mol Biol Evol. 2022 Dec 6:msac258. doi:

Inserts

ATP1A1
ATP1B1

Expression

Insect

Mutation

removed methionine at 122 in the protein sequence

Promoter

PH and p10

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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Chinchilla+N122D–insM

Plasmid

#191227

Purpose

Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

Depositor

Susanne Dobler

Article

Mohammadi et al Mol Biol Evol. 2022 Dec 6:msac258. doi:

Inserts

ATP1A1
ATP1B1

Expression

Insect

Mutation

removed methionine at 122 and replaced asparagine…

Promoter

PH and p10

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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Sandgrouse–insD

Plasmid

#191228

Purpose

Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

Depositor

Susanne Dobler

Article

Mohammadi et al Mol Biol Evol. 2022 Dec 6:msac258. doi:

Inserts

ATP1A1
ATP1B1

Expression

Insect

Mutation

removed aspartic acid at 121 in the protein seque…

Promoter

PH and p10

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-MBP-GGSG-DmBam_13-36_AG

Plasmid

#148841

Purpose

Mammalian Expression of DmBam_13-36

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

DmBam_13-36 (bam Fly)

Expression

Mammalian

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-HUWE1_UIM-mut

Plasmid

#187147

Purpose

Entry clone

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Entry clone

Mutation

A1376G/S1382A

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST_FLAG-HUWE1_F3194S

Plasmid

#187140

Purpose

mammalian cell expression of FLAG-HUWE1 F3194S

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Tags

1xFLAG

Expression

Mammalian

Mutation

F3194S

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pDARMO_CMVT_3xFLAG-HUWE1_H669Q

Plasmid

#187138

Purpose

mammalian cell expression of 3xFLAG-HUWE1 H669Q

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

H669Q

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pAC8-HUWE1-HECTonly

Plasmid

#187119

Purpose

insect cell expression of HUWE1 HECT domain

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Tags

Strep

Expression

Insect

Mutation

delta1-3992

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-HUWE1_UBA/UIM-mut

Plasmid

#187151

Purpose

Entry clone

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Entry clone

Mutation

M1328A/F1330A/A1376G/S1382A

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-HUWE1_UBM-mut

Plasmid

#187149

Purpose

Entry clone

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Entry clone

Mutation

L2976A/P2977A

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-HUWE1_UBA-mut

Plasmid

#187145

Purpose

Entry clone

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Entry clone

Mutation

M1328A/F1330A

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-HUWE1_BH3-4E

Plasmid

#187143

Purpose

Entry clone

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Entry clone

Mutation

V1976E/V1780E/L1983E/M1987E

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-HUWE1_delta169-189

Plasmid

#187134

Purpose

Entry clone

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Entry clone

Mutation

delta169-189

Available Since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-HUWE1_delta3896-3951

Plasmid

#187132

Purpose

Entry clone

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Entry clone

Mutation

delta3896-3951

Available Since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-HUWE1_I3969A/F3982A

Plasmid

#187128

Purpose

Entry clone

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Entry clone

Mutation

I3969A/F3982A

Available Since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-HUWE1_YH/GG_delta3291-3298

Plasmid

#187126

Purpose

Entry clone

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Entry clone

Mutation

Y355G/H356G, delta3291-3298

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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