We narrowed to 10,893 results for: ENA;

1. pLyGo-Bs-2

   Plasmid

   #163140

   Purpose

   pLyGo cloning vector for a sequence of interest (LPMO) in Bacillus subtilis. Vector encoding the AmyQ signal peptide and the LyGo cassette (SapI-ccdB-SapI)

   Depositor

   Morten Norholm

   Article

   Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PamyL-PamyQ-PcryIIIA-cryIIIAstab49

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLyGo-Ec-3

   Plasmid

   #163129

   Purpose

   pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding OmpA signal peptide and the LyGo cassette (SapI-ccdB-SapI)

   Depositor

   Morten Norholm

   Article

   Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   May 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLyGo-Ec-5

   Plasmid

   #163131

   Purpose

   pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding DsBA signal peptide and the LyGo cassette (SapI-ccdB-SapI)

   Depositor

   Morten Norholm

   Article

   Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   April 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. YCplac33-NAT_NDT1

   Plasmid

   #140593

   Purpose

   Express NDT1 in S. cerevisiae

   Depositor

   David Sabatini

   Article

   Kory et al Sci Adv. 2020 Oct 21;6(43):eabe5310. doi

   Insert

   NDT1 with 5' and 3' UTRs (YIA6 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   NDT1 promoter and 3'UTR

   Available Since

   Sept. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. sg2

   Plasmid

   #113967

   Purpose

   Single short guide RNA targeting TACCACATTTGTAGAGGTT

   Depositor

   Jordan Green

   Article

   Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

   Insert

   sg2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   May 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. sg3

   Plasmid

   #113968

   Purpose

   Single short guide RNA targeting CAATGTATCTTATCATGTC

   Depositor

   Jordan Green

   Article

   Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

   Insert

   sg3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   May 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. sg2+sg3

   Plasmid

   #113970

   Purpose

   Double short guide RNA targeting TACCACATTTGTAGAGGTT & CAATGTATCTTATCATGTC

   Depositor

   Jordan Green

   Article

   Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

   Insert

   sg2+sg3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   May 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYPQ290-D570A-SRDX

   Plasmid

   #129682

   Purpose

   Gateway entry clone for CRISPR-Cas12b systems

   Depositor

   Yiping Qi

   Article

   Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

   Insert

   AacCas12b-SRDX
   

   Use

   CRISPR; Gateway compatible aaccas12b entry clone

   Tags

   Expression

   Plant

   Mutation

   D570A; AacCas12b is rice codon optimized

   Promoter

   Available Since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYPQ291-D573A-SRDX

   Plasmid

   #129683

   Purpose

   Gateway entry clone for CRISPR-Cas12b systems

   Depositor

   Yiping Qi

   Article

   Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

   Insert

   BthCas12b-SRDX
   

   Use

   CRISPR; Gateway compatible bthcas12b entry clone

   Tags

   Expression

   Plant

   Mutation

   D573A; BthCas12b is rice codon optimized

   Promoter

   Available Since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEF1a-T2-GpNLuc

    Plasmid

    #135949

    Purpose

    Expresses the GpNLuc LumiFluor targeted to the mitochondira in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

    Insert

    GpNLuc LumiFluor
    

    Use

    Tags

    T2 mitochondrial localization sequence

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEF1a-T2-OgNLuc

    Plasmid

    #135951

    Purpose

    Expresses the OgNLuc LumiFluor targeted to the mitochondira in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

    Insert

    OgNLuc LumiFluor
    

    Use

    Tags

    T2 mitochondrial localization sequence

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAWH-IRP

    Plasmid

    #133896

    Purpose

    Positive control when used with pMS22H-IRE. Expression of Gal4AD-IRP hybrid protein. Homology regions for recombination with pMS22H

    Depositor

    Sebastian Maurer

    Article

    Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

    Insert

    IRP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    T7

    Available Since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLY151

    Plasmid

    #130947

    Purpose

    pspFΔHTH::λN22plus gene controlled by promoter PrhaB

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Inserts

    rhaS
    pspFΔHTH::λN22plus
    

    Use

    Synthetic Biology

    Tags

    pspFΔHTH::λN22plus

    Expression

    Bacterial

    Mutation

    The HTH domain of the pspF gene was deleted (297-…

    Promoter

    Pcon and PrhaB

    Available Since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1(+)/HP + GUG-nLuc-3XFLAG

    Plasmid

    #127323

    Purpose

    Express 3xFLAG tagged nanoLuciferase (nLuc) from GUG start codon from mRNA with hairpin in 5' UTR

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1(+)/AUU-nLuc-3XFLAG

    Plasmid

    #127305

    Purpose

    Express 3xFLAG tagged nanoLuciferase (nLuc) from AUU start codon

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1(+)/AAA-nLuc-3XFLAG

    Plasmid

    #127306

    Purpose

    Express 3xFLAG tagged nanoLuciferase (nLuc) from AAA start codon (Negative control plasmid)

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGL4.13/AUG(30)-FFLuc-3XFLAG

    Plasmid

    #127336

    Purpose

    Express truncated 3xFLAG tagged firefly luciferase (FFLuc) from AUG at codon 30

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    Firefly luciferase
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available Since

    June 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1(+)/AGG-nLuc-3XFLAG

    Plasmid

    #127304

    Purpose

    Express 3xFLAG tagged nanoLuciferase (nLuc) from AGG start codon

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1(+)/ACG-nLuc-3XFLAG

    Plasmid

    #127303

    Purpose

    Express 3xFLAG tagged nanoLuciferase (nLuc) from ACG start codon

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1(+)/GGG-nLuc-3XFLAG

    Plasmid

    #127307

    Purpose

    Express 3xFLAG tagged nanoLuciferase (nLuc) from GGG start codon (Negative control plasmid)

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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