We narrowed to 4,089 results for: PRS

1. Hsh155 Q773A R775A R776A Q778A (C4A)

   Plasmid

   #111946

   Purpose

   This is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has the Q773A R775A R776A Q778A mutation

   Depositor

   Aaron Hoskins

   Article

   Carrocci et al RNA. 2018 May 11. pii: rna.065664.118. d

   Insert

   Hsh155 Q773A R775A R776A Q778A (C4A)
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Q773A R775A R776A Q778A

   Promoter

   Available since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBW106

   Plasmid

   #111138

   Purpose

   For the expression of rat AANAT with Met-Ser-Ile N-terminus in S. cerevisiae

   Depositor

   Alexander Varshavsky

   Article

   Wadas et al J Biol Chem. 2016 Aug 12;291(33):17178-9

   Insert

   MSI-ratAANAT-3FLAG (Leu2 deletion) (Aanat Rat)
   

   Use

   Tags

   3x Flag

   Expression

   Yeast

   Mutation

   Leu2 deletion

   Promoter

   Gal1

   Available since

   June 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTCW018

   Plasmid

   #83531

   Purpose

   Aromatic amino acid inducible expression of alpha pheromone, and pheromone inducible exression of an ARO7 RNA interference hairpin

   Depositor

   Claudia Vickers

   Article

   Williams et al Metab Eng. 2015 May;29:124-134. doi: 10.

   Insert

   pARO9-mf_2-pFUS1J2-ARO7i-CYC1t
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pARO9/pFUS1J2

   Available since

   Oct. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pWHI2-HYG

   Plasmid

   #80589

   Purpose

   CEN plasmid expressing full-length WHI2 from S.cerevisiae S288C background under its native promoter

   Depositor

   John McCusker

   Article

   Vijayraghavan et al Genetics. 2018 Nov 29. pii: genetics.118

   Insert

   WHI2 (WHI2 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   WHI2 native promoter

   Available since

   July 28, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pHAGE NFkB-TA-LUC-UBC-GFP-W

   Plasmid

   #49343

   Purpose

   pHAGE NFkB-TA-LUC-UBC-GFP-W

   Depositor

   Darrell Kotton

   Article

   Wilson et al Mol Ther. 2013 Apr;21(4):825-33. doi: 10

   Inserts

   eGFP (eGFP )
   Luciferase
   NFkB consensus binding sequence
   TA
   UBC
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNCS mCherry

   Plasmid

   #91769

   Purpose

   Bacterial expression of a fluorescent protein, monomeric Cherry (mCherry)

   Depositor

   Erik Rodriguez, Roger Tsien

   Article

   Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

   Insert

   monomeric Cherry
   

   Use

   Tags

   Hexa-Histidine tag, Xpress epitope for detection …

   Expression

   Bacterial

   Mutation

   See Depositor Comments

   Promoter

   T7

   Available since

   June 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRK5-Myc-Parkin

   Plasmid

   #17612

   Depositor

   Ted Dawson

   Article

   Zhang et al Proc Natl Acad Sci U S A 2000 Nov 21;97(

   Insert

   Parkin (PRKN Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDuRCC-K

   Plasmid

   #81193

   Purpose

   Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Kan Resistance

   Depositor

   Eckhard Boles

   Article

   Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

   Inserts

   Cas9
   empty gRNA cassette
   empty gRNA cassette
   

   Use

   CRISPR

   Tags

   NTS

   Expression

   Yeast

   Mutation

   Please see depositor comments below., Please view…

   Promoter

   ROX3 and SNP52p

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAV106-pTDH3-mCherry-10xSumo-6xSIM

   Plasmid

   #116928

   Purpose

   Yeast integrative vector for expression of the SUMO-SIM complex

   Depositor

   Liam Holt

   Article

   Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

   Insert

   10xSUMO-6xSIM
   

   Use

   Tags

   mCherry

   Expression

   Yeast

   Mutation

   Promoter

   TDH3

   Available since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBMN-mCherry-Parkin

    Plasmid

    #59419

    Purpose

    to make mCherry-Parkin stable cell line with retrovirus

    Depositor

    Richard Youle

    Article

    Yamano et al Elife (Cambridge). 2014 Feb 25;3:e01612.

    Insert

    mCherry-Parkin (PRKN Human)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPFO*

    Plasmid

    #120400

    Purpose

    Expresses Perfringolysin O cholesterol sensor, has N-terminal His6-tag and three point mutations, A71T, C459A, and Y181A. Y181A prevents PFO's lytic activity at 4°C.

    Depositor

    Arun Radhakrishnan

    Article

    Das et al Proc Natl Acad Sci U S A. 2013 Jun 25;11

    Insert

    Mutant form of Perfringolysin O
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Amino acids 1-27 are deleted, starts at amino aci…

    Promoter

    Available since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSS146

    Plasmid

    #64770

    Purpose

    For integration of Cre recombinase at the yeast URA3 locus orthe CYC1 terminator

    Depositor

    Fred van Leeuwen

    Article

    Terweij et al G3 (Bethesda). 2013 Aug 7;3(8):1261-72.

    Insert

    Cre-EBD78
    

    Use

    Tags

    fused to the human Estrogen Binding Domain (EBD),…

    Expression

    Yeast

    Mutation

    Promoter

    TDH4

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBMN-YFP-Parkin

    Plasmid

    #59416

    Purpose

    to make YFP-Parkin stable cell line with retrovirus

    Depositor

    Richard Youle

    Article

    Yamano et al Elife (Cambridge). 2014 Feb 25;3:e01612.

    Insert

    YFP-Parkin (PRKN Human)
    

    Use

    Retroviral

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. siRNA-resistant ss-SigmaR1DTM-mNeonGreen R119A F191A

    Plasmid

    #226576

    Purpose

    Encodes mutant siRNA resistant SigmaR1 protein (transmembrane region deletion + substitutions) labeled with mNeonGreen

    Depositor

    Gia Voeltz

    Article

    Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

    Insert

    SigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    R119A, F191A

    Promoter

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-Parkin

    Plasmid

    #213547

    Purpose

    Transient mammalian expresssion of untagged Parkin

    Depositor

    Steven Altschuler, Lani Wu

    Article

    Waters et al Cell Rep. 2023 Oct 31;42(10):113260. doi

    Insert

    Parkin (PRKN Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. siRNA-resistant SigmaR1-mNeonGreen R119A F191A

    Plasmid

    #226574

    Purpose

    Encodes mutant siRNA resistant SigmaR1 protein (substitutions) labeled with mNeonGreen

    Depositor

    Gia Voeltz

    Article

    Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

    Insert

    SigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    R119A, F191A

    Promoter

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGFP-parkin C431S

    Plasmid

    #45877

    Depositor

    Edward Fon

    Article

    Trempe et al Science. 2013 May 9.

    Insert

    Parkin (PRKN Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    C431S mutation (ubiquitin acceptor site)

    Promoter

    CMV

    Available since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pHAGE hSPC-eGFP-W

    Plasmid

    #36450

    Depositor

    Darrell Kotton

    Article

    Longmire et al Cell Stem Cell. 2012 Apr 6;10(4):398-411

    Inserts

    human SP-C promoter
    eGFP (eGFP )
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    N/A and human SP-C promoter

    Available since

    May 11, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. Parkin

    Plasmid

    #187897

    Purpose

    Mammalian cell expression of untagged Parkin

    Depositor

    Erika Holzbaur

    Article

    Harding et al Mol Cell. 2023 Sep 7;83(17):3188-3204.e7

    Insert

    PRKN (PRKN Human)
    

    Use

    Tags

    None

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTW040

    Plasmid

    #64769

    Purpose

    For integration of Cre recombinase at the yeast HIS3 locus orthe CYC1 terminator

    Depositor

    Fred van Leeuwen

    Article

    Terweij et al G3 (Bethesda). 2013 Aug 7;3(8):1261-72.

    Insert

    Cre-EBD78
    

    Use

    Tags

    fused to the human Estrogen Binding Domain (EBD),…

    Expression

    Yeast

    Mutation

    Promoter

    TDH3

    Available since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge