We narrowed to 17,688 results for: SHE

1. pDual_dsCas9_Venus_hs_NC_chr2

   Plasmid

   #214683

   Purpose

   Lentiviral expression vector for an inducible Cas9-P2A-Venus with two sgRNA sequences against human non-coding DNA region of chromosome 2 (negative control)

   Depositor

   Kevin Janes

   Insert

   dgRNA_Chr2
   

   Use

   CRISPR and Lentiviral

   Available Since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDual_dsCas9_Venus_hs_TP73

   Plasmid

   #214685

   Purpose

   Lentiviral expression vector for an inducible Cas9-P2A-Venus with two sgRNA sequences against human TP73

   Depositor

   Kevin Janes

   Insert

   dgRNA_TP73 (TP73 Human)
   

   Use

   CRISPR and Lentiviral

   Available Since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDual_dsCas9_BFP_hs_NC_chr1

   Plasmid

   #214686

   Purpose

   Lentiviral expression vector for an inducible Cas9-P2A-BFP with two sgRNA sequences against human non-coding DNA region of chromosome 1 (negative control)

   Depositor

   Kevin Janes

   Insert

   dgRNA_Chr1
   

   Use

   CRISPR and Lentiviral

   Available Since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDual_dsCas9_BFP_hs_TP73

   Plasmid

   #214687

   Purpose

   Lentiviral expression vector for an inducible Cas9-P2A-BFP with two sgRNA sequences against human TP73

   Depositor

   Kevin Janes

   Insert

   dgRNA_TP73 (TP73 Human)
   

   Use

   CRISPR and Lentiviral

   Available Since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOliSpec5'

   Plasmid

   #228551

   Purpose

   Template for PCR amplification for gRNA cloning. The PCR product recombines in vivo with a PCRs products from pOliSpec3' and oligo-spacer amplifications, to clone gRNA plasmid

   Depositor

   Jeferson Gross

   Insert

   Truncated 5' region of aadA
   

   Use

   CRISPR

   Available Since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pOliSpec3'

   Plasmid

   #228550

   Purpose

   Template for PCR amplification for gRNA cloning. The PCR product recombines in vivo with a PCRs products from pOliSpec5' and oligo-spacer amplifications, to clone gRNA plasmid.

   Depositor

   Jeferson Gross

   Insert

   Truncated 3' region of aadA
   

   Use

   CRISPR

   Available Since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHR-TRE-miRFP703_PGK-Puro

   Plasmid

   #216667

   Purpose

   Expresses tTa-inducible miRFP703, with puromycin resistance

   Depositor

   Leonardo Morsut

   Inserts

   miRFP703
   PuroR
   

   Use

   Lentiviral

   Available Since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti puro HA-TUBA1B

   Plasmid

   #227587

   Purpose

   Lentiviral plasmid expressing human TUBA1B protein

   Depositor

   Addgene Research Program, Melina Fan

   Insert

   TUBA1B (TUBA1B Human)
   

   Use

   Lentiviral

   Tags

   HA

   Promoter

   CMV

   Available Since

   Nov. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-phSyn-flex-SypHaloTag

   Plasmid

   #172280

   Purpose

   Cre-dependent HaloTag labeling of neuronal presynaptic terminals from the synapsin promoter.

   Depositor

   Danny Winder

   Insert

   synaptophysin-HaloTag
   

   Use

   AAV and Cre/Lox

   Tags

   HaloTag

   Expression

   Mammalian

   Promoter

   rat synapsin

   Available Since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. MDH2_EC

    Plasmid

    #224146

    Purpose

    IPTG induces expression of C. elegans protein MDH-2 with a N-terminal His tag.

    Depositor

    Katherine Walstrom

    Insert

    MDH-2 (mdh-2 Nematode)
    

    Tags

    His tag, Xpress tag, enterokinase cleaveage site

    Expression

    Bacterial

    Available Since

    Sept. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pIND4-GFP-MmXYRS

    Plasmid

    #222745

    Purpose

    Expresses sfGFP and aaRS-tRNA pair for incorporating haloalkane crosslinker

    Depositor

    Steven Boxer

    Inserts

    sfGFP
    MmXYRS
    Mm Pyl tRNA
    

    Tags

    6xHis

    Mutation

    Contains the following substitutions: V346A, W348…

    Promoter

    rrnB

    Available Since

    Sept. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. AiP1999 - pAAV-AiE2255m-minBG-iCre(R297T)-BGHpA

    Plasmid

    #223843

    Purpose

    Enhancer-driven expression of the iCre(R297T) recombinase in thalamic PVT neurons

    Depositor

    Bosiljka Tasic

    Insert

    iCre(R297T)
    

    Use

    AAV

    Mutation

    Argenine in aa297 was replaced with Threonine

    Promoter

    minBG

    Available Since

    Aug. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. H2B-Electra2

    Plasmid

    #218929

    Purpose

    Electra2 fused to human H2B protein for nuclear localization

    Depositor

    Amro Hamdoun

    Insert

    H2B-Electra2 (H2B Entacmaea quadricolor, Human, Synthetic)
    

    Use

    Synthetic Biology

    Tags

    Electra2 and H2B

    Expression

    Mammalian

    Available Since

    Aug. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. LvPolyUb

    Plasmid

    #218982

    Purpose

    L.variegatus polyubiquitin-C promoter

    Depositor

    Amro Hamdoun

    Insert

    L.variegatus polyubiquitinC promoter
    

    Use

    Sea urchin

    Promoter

    L.variegatus polyubiquitinC promoter

    Available Since

    Aug. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pet28a+_Yjjg

    Plasmid

    #220228

    Purpose

    Bacterial expression of Yjjgin E. coli BL21_DE3 (pLys) or similar

    Depositor

    Bernd Nidetzky

    Insert

    Pyrimidine 5'-nucleotidase YjjG (yjjG E.coli)
    

    Tags

    Histag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. MUT_S1-spacer-H1

    Plasmid

    #218399

    Purpose

    3-exon minigene, with SRSF1 motif-rich middle exon, hnRNPA1 motif-rich region in second intron following a neutral spacer, and AG>GG mutation in final 3' splice site

    Depositor

    Oded Regev

    Insert

    Modified CHO DHFR minigene
    

    Expression

    Mammalian

    Promoter

    tetracycline-responsive promoter

    Available Since

    June 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. MUT_S1-H1,NR

    Plasmid

    #218395

    Purpose

    3-exon minigene, with SRSF1 motif-rich middle exon, hnRNPA1 motif-rich region in second intron, both randomly mutated to avoid sequence repeats, and AG>GG mutation in final 3' splice site

    Depositor

    Oded Regev

    Insert

    Modified CHO DHFR minigene
    

    Expression

    Mammalian

    Promoter

    tetracycline-responsive promoter

    Available Since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. 1167A

    Plasmid

    #218233

    Purpose

    HDR plasmid for inserting Ceratitis capitata transformer female intron into the white pupae gene

    Depositor

    Omar Akbari

    Insert

    putative metabolite transport protein
    

    Expression

    Insect

    Available Since

    May 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28-Flag-RBM24

    Plasmid

    #208621

    Purpose

    for E. coli expression of Flag tagged human RBM24 (ENST00000379052)

    Depositor

    Quanfu Ma

    Insert

    human RBM24 (ENST00000379052) coding region (RBM24 Human)
    

    Tags

    6xHis and Flag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET-29b(+)_notufA-EmEndoF2-CBD-His

    Plasmid

    #218197

    Purpose

    Bacterial expression of a His-tagged Chitin Binding fusion of Elizabethkingia miricola EndoF2

    Depositor

    Florence Pojer

    Insert

    EndoF2
    

    Tags

    CBD-His

    Expression

    Bacterial

    Mutation

    codon optimized Ecoli, residues 45-335

    Promoter

    T7

    Available Since

    April 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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21. pAG426-GAL-Prepro-3x-Beta-Amyloid

    Plasmid

    #181730

    Purpose

    Galactose inducible expression of Prepro-3x-Beta-Amyloid

    Depositor

    Alejandro Chavez

    Insert

    Prepro-3x-Beta-Amyloid
    

    Expression

    Yeast

    Available Since

    April 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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22. E3_pENTR_L3-L2_NI_G1333-DddA11-N

    Plasmid

    #191609

    Purpose

    ptpTALECD_v2mod vector assembly, Entry clone 1 with attL2 and L3

    Depositor

    Shin-ichi Arimura

    Insert

    C- and N termini of platinum TALEN, a half of the evolved DddA (DddA11), and a uracil glycosylase inhibitor
    

    Use

    Other

    Available Since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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23. E3_pENTR_L3-L2_NN_G1333-DddA11-N

    Plasmid

    #191610

    Purpose

    ptpTALECD_v2mod vector assembly, Entry clone 1 with attL2 and L3

    Depositor

    Shin-ichi Arimura

    Insert

    C- and N termini of platinum TALEN, a half of the evolved DddA (DddA11), and a uracil glycosylase inhibitor
    

    Use

    Other

    Available Since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. E1_pENTR_L1-L4_HD_G1333-DddA11-C

    Plasmid

    #191599

    Purpose

    ptpTALECD_v2mod vector assembly, Entry clone 1 with attL1 and L4

    Depositor

    Shin-ichi Arimura

    Insert

    C- and N termini of platinum TALEN, a half of the evolved DddA (DddA11), and a uracil glycosylase inhibitor
    

    Use

    Other

    Available Since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. E1_pENTR_L1-L4_NG_G1333-DddA11-C

    Plasmid

    #191600

    Purpose

    ptpTALECD_v2mod vector assembly, Entry clone 1 with attL1 and L4

    Depositor

    Shin-ichi Arimura

    Insert

    C- and N termini of platinum TALEN, a half of the evolved DddA (DddA11), and a uracil glycosylase inhibitor
    

    Use

    Other

    Available Since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. E1_pENTR_L1-L4_NI_G1333-DddA11-C

    Plasmid

    #191601

    Purpose

    ptpTALECD_v2mod vector assembly, Entry clone 1 with attL1 and L4

    Depositor

    Shin-ichi Arimura

    Insert

    C- and N termini of platinum TALEN, a half of the evolved DddA (DddA11), and a uracil glycosylase inhibitor
    

    Use

    Other

    Available Since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. E1_pENTR_L1-L4_NN_G1333-DddA11-C

    Plasmid

    #191602

    Purpose

    ptpTALECD_v2mod vector assembly, Entry clone 1 with attL1 and L4

    Depositor

    Shin-ichi Arimura

    Insert

    C- and N termini of platinum TALEN, a half of the evolved DddA (DddA11), and a uracil glycosylase inhibitor
    

    Use

    Other

    Available Since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. E3_pENTR_L3-L2_HD_G1333-DddA11-N

    Plasmid

    #191607

    Purpose

    ptpTALECD_v2mod vector assembly, Entry clone 1 with attL2 and L3

    Depositor

    Shin-ichi Arimura

    Insert

    C- and N termini of platinum TALEN, a half of the evolved DddA (DddA11), and a uracil glycosylase inhibitor
    

    Use

    Other

    Available Since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. E3_pENTR_L3-L2_NG_G1333-DddA11-N

    Plasmid

    #191608

    Purpose

    ptpTALECD_v2mod vector assembly, Entry clone 1 with attL2 and L3

    Depositor

    Shin-ichi Arimura

    Insert

    C- and N termini of platinum TALEN, a half of the evolved DddA (DddA11), and a uracil glycosylase inhibitor
    

    Use

    Other

    Available Since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pT7CFE1_polio_VP12A

    Plasmid

    #212988

    Purpose

    Encodes Poliovirus type I Mahoney VP12A optimized for expression in mammalian transcription/translation extracts

    Depositor

    Karla Kirkegaard

    Insert

    Poliovirus type I Mahoney VP12A
    

    Use

    Mammalian cell free protein expression

    Tags

    HA tag

    Promoter

    T7

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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