We narrowed to 8,316 results for: Set

1. PCT62

   Plasmid

   #170391

   Purpose

   expresses three sgRNA cassettes

   Depositor

   Jonathan Weissman

   Article

   Quinn et al Science. 2021 Feb 26;371(6532). pii: sci

   Insert

   PCT62-targetsite
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-UBC-mARG3

   Plasmid

   #173793

   Purpose

   Mammalian acoustic reporter gene 1 cassette 3 on PB-UBC plasmid

   Depositor

   Xiaojun Lian

   Article

   Howells et al Bioeng Transl Med. 2023 Aug 2;9(2):e1058

   Insert

   GvpJ, F, G, L, K
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   UBC promoter

   Available since

   July 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a_SUMO_AcCas12n

   Plasmid

   #203808

   Purpose

   pET28a-based plasmid containing a T7-driven His6-SUMO-HRV3c-AcCas12n cassette. Kanamycin resistance.

   Depositor

   Quanjiang Ji

   Article

   Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

   Insert

   SUMO_AcCas12n
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Aug. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pT2ST

   Plasmid

   #59384

   Purpose

   Template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a trimethoprim resistance gene.

   Depositor

   Shelley Copley

   Article

   Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

   Insert

   TT-ISceI-dfrA
   

   Use

   Template

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDGB3_alpha1-AtUBQ10:HR1b-GFP-T35S

   Plasmid

   #211837

   Purpose

   Plant transient expression cassette for HR1b-GFP fusion under UBQ10 promoter.

   Depositor

   Marcela Rojas-Pierce

   Article

   Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

   Insert

   HR1b
   

   Use

   Synthetic Biology

   Tags

   GFP

   Expression

   Plant

   Mutation

   Promoter

   UBIQUITIN10

   Available since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSP618

   Plasmid

   #169235

   Purpose

   Spear-ATAC lentiviral backbone - includes a mU6-sgRNA cassette with flanking Nextera sequencing adapters

   Depositor

   William Greenleaf

   Article

   Pierce et al Nat Commun. 2021 May 20;12(1):2969. doi:

   Insert

   mU6-sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTmpGm

   Plasmid

   #176229

   Purpose

   A template plasmid for amplification of the cI-hok-aacC4 cassette

   Depositor

   Sergey Sineoky

   Article

   Bubnov et al Nucleic Acids Res. 2022 Aug 26;50(15):89

   Insert

   cI of phage lambda, hok of the R1 plasmid, aacC4
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLCE-P2A-Puro

   Plasmid

   #123318

   Purpose

   Lentiviral vector for expression of eGFP-P2A-Puro cassette from a CMV promoter

   Depositor

   Eva Gottwein

   Article

   Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 1

   Insert

   eGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPB-R1R2-EM7neoPheS

   Plasmid

   #112923

   Purpose

   piggyBac Transposon vector with a Gateway destination cassette (neoPheS selection)

   Depositor

   Kosuke Yusa

   Article

   Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

   Insert

   neoPheS
   

   Use

   Piggybac transposon gateway destination vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. RP4-11

    Plasmid

    #79812

    Purpose

    Modified RP4 plasmid with Km resistance and sacB gene. Easily curable helper plasmid

    Depositor

    Michael Hynes

    Article

    Quandt et al Plasmid. 2004 Jul;52(1):1-12.

    Insert

    Kanamycin cassette and SacB gene (sacB )
    

    Use

    Helper plasmid for mobilization

    Tags

    Expression

    Mutation

    Promoter

    Km

    Available since

    Aug. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pCfB8644

    Plasmid

    #126919

    Purpose

    Plasmid containing integration cassette at IntC2 integration site in Yarrowia lipolytica for overexpression of XdCrtI and XdCrtYB

    Depositor

    Irina Borodina

    Article

    Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

    Insert

    XdCrtI and HMG1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJ204-loxP-100RPU.2-ETN-lox2272

    Plasmid

    #154833

    Purpose

    Donor plasmid for Cre/lox-based recombinase-mediated cassette exchange (RMCE), expressing one copy of etanercept

    Depositor

    Lise Marie Grav

    Article

    Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

    Insert

    ETN
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    100RPU.2

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAGD289

    Plasmid

    #228113

    Purpose

    pET-21(+) deriviative with retron Kva1 containing a donor to edit E. coli rpoB gene cloned downstream of a T7 promoter

    Depositor

    Seth Shipman

    Article

    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

    Insert

    Kva1 retron, ncRNA with 90 bp rpoB donor
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSW071

    Plasmid

    #140037

    Purpose

    PCC 11901 fadD locus KO with a kanamycin resistance cassette

    Depositor

    Peter Nixon

    Article

    Wlodarczyk et al Commun Biol. 2020 May 7;3(1):215. doi: 1

    Insert

    Kanamycin resistance gene
    

    Use

    Gene knockout in cyanobacteria

    Tags

    Expression

    Mutation

    Promoter

    KanR

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCfB8787

    Plasmid

    #126921

    Purpose

    Plasmid containing integration cassette at IntD1 integration site in Yarrowia lipolytica for overexpression of HMG1

    Depositor

    Irina Borodina

    Article

    Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

    Insert

    HMG1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCfB8748

    Plasmid

    #126918

    Purpose

    Plasmid containing integration cassette at IntE1 integration site in Yarrowia lipolytica for overexpression of XdCrtE

    Depositor

    Irina Borodina

    Article

    Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

    Insert

    XdCrtE
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBTK622

    Plasmid

    #180592

    Purpose

    tdk/kanR dual selection cassette for engineering the genome of Acinetobacter baylyi ADP1

    Depositor

    Jeffrey Barrick

    Article

    Suarez et al Nucleic Acids Res. 2020 May 7;48(8):4585

    Inserts

    tdk
    kanR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRGD-TcR

    Plasmid

    #74110

    Purpose

    Plasmid containing tetracycline resistance gene cassette flanked by multiple restriction sites

    Depositor

    Hans-Martin Fischer

    Article

    Ledermann et al Appl Environ Microbiol. 2016 Feb 26. pii

    Insert

    tetA(C)
    

    Use

    Bacterial vector based on pmb1 replicon

    Tags

    Expression

    Mutation

    C-to-T transition at pos. no. 1049 of tetA(C) lea…

    Promoter

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJH136

    Plasmid

    #47554

    Purpose

    pJH136 carries a URA3 cassette flanked by PCR priming sites U2 and D2. URA3 was derived from S288c and extends from 243 bp upstream of the start codon to 79 bp downstream of the stop codon.

    Depositor

    Ronald Davis

    Article

    Horecka et al Yeast. 2014 Mar;31(3):103-12. doi: 10.10

    Insert

    URA3
    

    Use

    Pcr template

    Tags

    Expression

    Mutation

    Promoter

    Native URA3 promoter, -243 to -1.

    Available since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCfB8788

    Plasmid

    #126922

    Purpose

    Plasmid containing integration cassette at IntD1 integration site in Yarrowia lipolytica for overexpression of XdCrtI and HMG1

    Depositor

    Irina Borodina

    Article

    Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

    Insert

    XdCrtI and HMG1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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21. pSEED-mGreenLantern

    Plasmid

    #219643

    Purpose

    Permits GoldenGate assembly of donor plasmids (BsmBI) to knock-in mGreenLantern using the SEED/Harvest method

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    mGreenLantern SEED Cassette
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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22. ptRNAfMet-A37

    Plasmid

    #196657

    Purpose

    Plasmid for overexpression of native initiator tRNA (A37)

    Depositor

    Markus Jeschek

    Article

    Hollerer et al Nucleic Acids Res. 2023 Mar 21;51(5):237

    Insert

    tRNAfMet-A37 expression cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    metY native promoter

    Available since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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23. pKAd5f11pES-PmeI

    Plasmid

    #122698

    Purpose

    To generate an E1/E3-deleted human adenovirus 5 (HAdV-5) bearing a chimeric fiber and the exogenous gene expression cassette.

    Depositor

    Zhuo-Zhuang Lu

    Article

    Liu et al Hum Gene Ther. 2019 Feb 22. doi: 10.1089

    Type

    Empty backbone

    Use

    Adenoviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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24. pCfB8786

    Plasmid

    #126920

    Purpose

    Plasmid containing integration cassette at IntD1 integration site in Yarrowia lipolytica for overexpression of XdCrtI

    Depositor

    Irina Borodina

    Article

    Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

    Insert

    XdCrtI
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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25. OCT4-2A-mOrange

    Plasmid

    #66986

    Purpose

    Generate OCT4 mOrange reporter line without selection cassette

    Depositor

    Danwei Huangfu

    Article

    Zhu et al Stem Cell Reports. 2015 May 28. pii: S22

    Insert

    2A-mOrange
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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26. pRS-QUE7mu

    Plasmid

    #129311

    Purpose

    Low concentration ATP measurement

    Depositor

    Hiroyuki Noji

    Article

    Yaginuma et al Sci Rep. 2014 Oct 6;4:6522. doi: 10.1038

    Insert

    QUEEN-7mu
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pT2SK

    Plasmid

    #59383

    Purpose

    Template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a kanamycin resistance gene.

    Depositor

    Shelley Copley

    Article

    Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

    Insert

    TT-ISceI-kan
    

    Use

    Template

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pT2SC

    Plasmid

    #59382

    Purpose

    Template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a chloramphenicol resistance gene.

    Depositor

    Shelley Copley

    Article

    Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

    Insert

    TT-ISceI-cat
    

    Use

    Template

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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29. pBBK43 pHomL-TDH3p-GFPdropout-SSA1t-HomR (AmpR)

    Plasmid

    #179027

    Purpose

    Parent vector for markerless yeast integration cassettes with untagged genes

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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30. pME-FlEx switch

    Plasmid

    #82406

    Purpose

    Gateway-compatible FlEx cassette containing two pairs of heterotypic, antiparallel loxP-type recombination sites

    Depositor

    Judith Eisen

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge