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pcDNA4/HisMaxB-YAP1-S236A

Plasmid

#18991

Depositor

Marius Sudol

Article

Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

Insert

Yes-kinase associated protein S236A mutant (YAP1 Human)

Tags

His

Expression

Mammalian

Mutation

Serine 236 changed to Alanine

Available Since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
huCofilin WT SSR

Plasmid

#252616

Purpose

Expresses human wild-type cofilin (shRNA-resistant)

Depositor

James Bamburg

Article

Wiggan et al Dev Cell. 2012 Mar 13;22(3):530-43. doi:

Insert

cofilin 1 (CFL1 Human)

Expression

Mammalian

Mutation

silent mutations at nt 67 to 75 in which TCTTCAAC…

Promoter

CMV

Available Since

March 5, 2026

Availability

Industry, Academic Institutions, and Nonprofits

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ePB-Puro_TT-hCDX2-P2A-tdTomato-CAAX

Plasmid

#252249

Purpose

A PiggyBac donor vector for Dox-inducible expression of CDX2 and membrane-localized tdTomato.

Depositor

Jun Wu

Article

Oura et al Cell Stem Cell. 2025 Oct 2;32(10):1509-1

Insert

CDX2 (CDX2 Human)

Use

Piggybac transposon donor vector

Tags

P2A-tdTomato-CAAX

Expression

Mammalian

Promoter

tight TRE

Available Since

March 2, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-N-ALFA-bRho-EGFP

Plasmid

#248792

Purpose

Expresses ALFA-bRho-EGFP in mammalian cells. Modified from Addgene plasmid #45399

Depositor

Michael Robichaux

Article

Hekmatara et al bioRxiv [Preprint]. 2025 Sep 25:2025.09.

Insert

RHO (RHO Bovine)

Tags

ALFA and EGFP

Expression

Mammalian

Available Since

Jan. 22, 2026

Availability

Academic Institutions and Nonprofits only

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NanoBRET Assay, TEAD2 / YAP Bidirectional Vector

Plasmid

#238669

Purpose

Express TEAD2 / YAP Fusion Proteins in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Inserts

TEAD2 (TEAD2 Human)
YAP (YAP1 Human)

Tags

HaloTag (R) and NanoLuc (R)

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

Jan. 20, 2026

Availability

Industry, Academic Institutions, and Nonprofits

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pAvA143

Plasmid

#248147

Purpose

mutated LuxR transcriptional activator for expression in yeast: fused with Gal4 activation domain with 'gen2' mutations

Depositor

Emil Jensen

Article

van Aalst et al Commun Biol. 2025 Nov 27. doi: 10.1038/s

Insert

luxR (gen3 mutations)

Use

Integration vector

Tags

Gal4 activation domain and nuclear localization s…

Expression

Yeast

Mutation

Gal4_AD: F51 (TTC→TTT), G88E. LuxR: K53E, N86Y, I…

Promoter

pPGK1

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1/YAP-mScarlet3-H

Plasmid

#249171

Purpose

Mammalian expression vector of YAP fused with mScarlet3-H

Depositor

Pakorn Kanchanawong

Article

Nishimura et al J Cell Sci. 2025 Nov 24:jcs.264110. doi:

Insert

YAP (Yap1 Mouse)

Tags

mScarlet3-H

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/CALR wtΔKDEL YD/FL

Plasmid

#214805

Purpose

Mammalian expression of human CALR wtΔKDEL YD/FL

Depositor

Norio Komatsu

Article

Masubuchi et al Haematologica. 2025 Nov 6. doi: 10.3324/

Insert

CALR wtΔKDEL YD/FL (CALR Human)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pFastBac1/CALR ins5-His

Plasmid

#214796

Purpose

Baculovirus expression of human CALR ins5

Depositor

Norio Komatsu

Article

Masubuchi et al Haematologica. 2025 Nov 6. doi: 10.3324/

Insert

human CALR ins5 (CALR Human)

Tags

His

Expression

Insect

Available Since

Dec. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/CALR wt

Plasmid

#214797

Purpose

Mammalian expression of human CALR wt

Depositor

Norio Komatsu

Article

Masubuchi et al Haematologica. 2025 Nov 6. doi: 10.3324/

Insert

human CALR wt (CALR Human)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/CALR del52

Plasmid

#214798

Purpose

Mammalian expression of human CALR del52

Depositor

Norio Komatsu

Article

Masubuchi et al Haematologica. 2025 Nov 6. doi: 10.3324/

Insert

human CALR del52 (CALR Human)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/CALR ins5

Plasmid

#214799

Purpose

Mammalian expression of human CALR ins5

Depositor

Norio Komatsu

Article

Masubuchi et al Haematologica. 2025 Nov 6. doi: 10.3324/

Insert

human CALR ins5 (CALR Human)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/CALR WTΔKDEL

Plasmid

#214790

Purpose

Mammalian expression of human CALR wtΔKDEL

Depositor

Norio Komatsu

Article

Masubuchi et al Haematologica. 2025 Nov 6. doi: 10.3324/

Insert

human CALR wtΔKDEL (CALR Human)

Tags

non

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pGCas9_scr

Plasmid

#207567

Purpose

GeoCas9-mediated cleavage of genomic DNA in E. coli

Depositor

John van der Oost

Article

Trasanidou et al Commun Biol. 2023 Oct 13;6(1):1042. doi:

Insert

Plac/tet_GeoCas9_PlacI_lacI_PJ23119_sgRNA(scrambled non-targeting spacer)

Expression

Bacterial

Mutation

Wild-type

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pdTCas9_scr

Plasmid

#207568

Purpose

dThermoCas9-mediated silencing of genomic gene in E. coli

Depositor

John van der Oost

Article

Trasanidou et al Commun Biol. 2023 Oct 13;6(1):1042. doi:

Insert

Plac/tet_dThermoCas9_PlacI_lacI_PJ23119_sgRNA(scrambled non-targeting spacer)

Expression

Bacterial

Mutation

ThermoCas9(D8A,H582A)

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pdGCas9_scr

Plasmid

#207569

Purpose

dGeoCas9-mediated silencing of genomic gene in E. coli

Depositor

John van der Oost

Article

Trasanidou et al Commun Biol. 2023 Oct 13;6(1):1042. doi:

Insert

Plac/tet_dGeoCas9_PlacI_lacI_PJ23119_sgRNA(scrambled non-targeting spacer)

Expression

Bacterial

Mutation

GeoCas9(D8A,H582A)

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pAcrTCas9_scr

Plasmid

#207571

Purpose

AcrIIC1-mediated inhibition of ThermoCas9 cleavage activity in E. coli

Depositor

John van der Oost

Article

Trasanidou et al Commun Biol. 2023 Oct 13;6(1):1042. doi:

Insert

Prha_AcrIIC1Nme_Plac/tet_ThermoCas9_PlacI_lacI_PJ23119_sgRNA(scrambled non-targeting spacer)

Expression

Bacterial

Mutation

Wild-type

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pAcrGCas9_scr

Plasmid

#207572

Purpose

AcrIIC1-mediated inhibition of GeoCas9 cleavage activity in E. coli

Depositor

John van der Oost

Article

Trasanidou et al Commun Biol. 2023 Oct 13;6(1):1042. doi:

Insert

Prha_AcrIIC1Nme_Plac/tet_GeoCas9_PlacI_lacI_PJ23119_sgRNA(scrambled non-targeting spacer)

Expression

Bacterial

Mutation

Wild-type

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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mVenus-synaptojanin1(498-901)-CAAX

Plasmid

#241303

Purpose

Mammalian expression of truncated form of synaptojanin-1 with CAAX motif fused to mVenus

Depositor

Justin Taraska

Article

Obashi et al Structure. Volume 34, Issue 1, 8 January

Insert

Synaptojanin-1 (SYNJ1 Human)

Tags

mVenus

Expression

Mammalian

Mutation

498-901 aa

Promoter

CMV

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pX459-Cd109 gRNA

Plasmid

#246571

Purpose

CRISPR vector co-expressing Cas9 and a mouse Cd109 gRNA

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

Cd109 gRNA (Cd109 Mouse)

Use

CRISPR

Promoter

U6

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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