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Showing: 3921 - 3940 of 7413 results

  2. Mut2-RS

    Plasmid
    #182882
    Purpose
    A pEvol-based plasmid containing one inducible (by arabinose) copy of the Mut2-RS aaRS. The 2nd constitutive aaRS copy typically found in pEvol plasmids was deleted.
    Depositor
    Miriam Amiram
    Article
    Hadar et al Chembiochem. 2021 Apr 16;22(8):1379-1384
    Insert
    Mut2-RS
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    182882-plasmid-map-sequence-id-366061

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  6. pGBW-m4046852

    Plasmid
    #145584
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 nsp8
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 nsp8 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 28, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145584-plasmid-map-sequence-id-292508

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  7. pGL3-LRF

    Plasmid
    #66936
    Purpose
    Destination vector; gateway cassette cloned upstream of basal promoter driving luciferase. Forward orientation (attR1, attR2).
    Depositor
    Alvaro Monteiro
    Article
    Buckley et al Nat Protoc. 2016 Jan;11(1):46-60. doi: 1
    Type
    Empty backbone
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SV-40
    Available since
    Aug. 20, 2015
    Availability
    Academic Institutions and Nonprofits only

  9. pTNSQCas

    Plasmid
    #219434
    Purpose
    CRISPR-associated transposases from V. cholerae and used for RNA guided cargo insertion in Vibrio natriegens
    Depositor
    Sheng Yang
    Article
    Xu et al Biotechnol Bioeng. 2024 Mar;121(3):1163-
    Insert
    TnsA, TnsB, TnsC, TniQ, Cas8, Cas7, Cas6
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    219434-plasmid-map-sequence-id-435610

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  13. pAC-crRNA-Cm

    Plasmid
    #158711
    Purpose
    Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells and a non-targeting crRNA
    Depositor
    Yi-Cheng Sun
    Article
    Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1
    Insert
    eforCP (for crRNA cloning)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    TetO
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    158711-plasmid-map-sequence-id-325466

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  17. pCB-CBEv2

    Plasmid
    #221139
    Purpose
    Coxiella burnetii CRISPR-Cas9 cytosine base editing plasmid version 2 without sgRNA construct. Expresses 3xF-BE4-PpAPOBEC1(H122A).
    Depositor
    Craig Roy
    Article
    Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1
    Insert
    3xF-PpAPOBEC1(H122A)-Cas9n-UGI-UGI (BE4-PpAPOBEC1(H122A))
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Bacterial
    Mutation
    Promoter
    lacIq-Ptac
    Available since
    Nov. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    221139-plasmid-map-sequence-id-437031

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Showing: 3921 - 3940 of 7413 results