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pAAV-FLEX-hGTB

Plasmid

#26196

Depositor

Edward Callaway

Article

Wall et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

Insert

Tet transactivator, enhanced green fluorescent protein, TVA, rabies B19 glycoprotein

Use

AAV and Cre/Lox; Adeno-associated virus

Mutation

F2A has 2A cleavage site deleted, producing htTA-…

Available Since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only

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pF149 pSOD1G85RAcGFP1

Plasmid

#26410

Depositor

Elizabeth Fisher

Article

Stevens et al PLoS One. 2010 . 5(3):e9541.

Insert

SOD1 (SOD1 Human)

Tags

GFP

Expression

Mammalian

Mutation

G85R

Available Since

Nov. 8, 2010

Availability

Academic Institutions and Nonprofits only
pMIND

Plasmid

#24730

Depositor

Brian Robertson

Article

Blokpoel et al Nucleic Acids Res. 2005 . 33(2):e22.

Insert

TetRO promoter

Expression

Bacterial

Available Since

Aug. 4, 2010

Availability

Academic Institutions and Nonprofits only

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UbG76V-eGFP-V5His_20aa

Plasmid

#23970

Depositor

Nico Dantuma

Article

Beskow et al J Mol Biol. 2009 Dec 11. 394(4):732-46.

Insert

Ub-G76V-eGFP-V5HIS_20aa

Tags

eGFP

Expression

Mammalian

Mutation

G76V mutation in Ubiquitin. Ub fused to N-termin…

Available Since

March 22, 2010

Availability

Academic Institutions and Nonprofits only

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pDsRed-Sensor

Plasmid

#22743

Depositor

Lynn Hudson

Article

Lau et al J Neurosci. 2008 Nov 5. 28(45):11720-30.

Insert

modified pCMV-DsRed-Express (Clontech)

Expression

Mammalian

Available Since

Dec. 18, 2009

Availability

Academic Institutions and Nonprofits only

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GFP-N2-PKCgamma

Plasmid

#21204

Depositor

Tobias Meyer

Article

Oancea et al J Cell Biol. 1998 Feb 9. 140(3):485-98.

Insert

PKC gamma (Prkcg Rat)

Tags

GFP

Expression

Mammalian

Mutation

PKC gamma (rat). (Lab plasmid WC 0013)

Available Since

July 15, 2009

Availability

Academic Institutions and Nonprofits only

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pEX-CMV-SP-CFP-STM2(15-746)

Plasmid

#18863

Depositor

Tobias Meyer

Article

Brandman et al Cell. 2007 Dec 28. 131(7):1327-39.

Insert

Stromal Interaction Molecule 2 (STIM2 Human)

Tags

CFP

Expression

Mammalian

Mutation

CFP inserted between signal peptide and residues …

Available Since

March 24, 2009

Availability

Academic Institutions and Nonprofits only

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pCM1.57

Plasmid

#17252

Depositor

Geraldine Seydoux

Article

Merritt et al WormBook. 2010 Feb 8:1-21. doi: 10.1895/

Insert

L3788 promoter NO ATG in pDONRP4P1R

Use

Gateway Cloning

Available Since

Feb. 26, 2008

Availability

Academic Institutions and Nonprofits only

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pN1-PinkyCaMP

Plasmid

#232861

Purpose

A vector expressing the mScarlet based calcium indicator PinkyCaMP under CMV promoter

Depositor

Olivia Masseck

Article

Fink et al Nat Methods. 2026 Apr 24. doi: 10.1038/s

Insert

PinkyCaMP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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Cys-60nmSAH-SPYcatcher

Plasmid

#214840

Purpose

This plasmid encodes a protein (Cys-60nmSAH-SPYcatcher) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

Depositor

Hironori Funabiki

Article

Arimura et al eLife. 2025 May 20;13:RP103486. doi: 10.

Insert

Cys-60nmSAH-SPYcatcher

Tags

His-TEV-SUMO-

Expression

Bacterial

Promoter

T5

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pGD-P1/HC-Pro

Plasmid

#196328

Purpose

35S promoter-driven expression of the tobacco etch virus P1/HC-Pro RNA silencing suppressor

Depositor

Zhenghe Li

Article

Ganesan et al J Virol. 2013 Oct;87(19):10598-611. doi:

Insert

tobacco etch virus P1/HC-Pro

Expression

Plant

Promoter

duplicated cauliflower mosaic virus 35S promoter

Available Since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pEB3-mScarlet3_N1

Plasmid

#189770

Purpose

Construct for labelling microtubule plus ends in mammalian cells with EB3-mScarlet3

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

EB3-mScarlet3

Expression

Mammalian

Promoter

CMV

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_DNAseI

Plasmid

#182408

Purpose

Bacterial expression of DNaseI

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

DNase I (DNASE1 Bovine)

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pET N6his GFP1-9

Plasmid

#182240

Purpose

Tripartite split-GFP. E. coli vector. Expression of GFP1-9 detector fragment.

Depositor

Stéphanie Cabantous

Article

Pedelacq et al Methods Mol Biol. 2019;2025:423-437. doi

Insert

GFP1-9

Tags

6HIS

Expression

Bacterial

Promoter

T7

Available Since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-uvsX-H6

Plasmid

#163913

Purpose

Expresses uvsX for bacterial expression and affinity purification

Depositor

Paul Freemont

Article

Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

Insert

uvsX

Tags

His

Expression

Bacterial

Promoter

T7 promoter

Available Since

March 21, 2022

Availability

Academic Institutions and Nonprofits only

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pML433

Plasmid

#177190

Purpose

Expresses recombinant HyPro enzyme in E. coli

Depositor

Eugene Makeyev

Article

Yap et al Mol Cell. 2021 Nov 2. pii: S1097-2765(21

Insert

HyPro enzyme

Expression

Bacterial

Promoter

T7

Available Since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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NM9-SpCas9-NLS3

Plasmid

#128177

Purpose

Expresses R. toruloides codon-optimized SpCas9 under PGK1 promoter

Depositor

Huimin Zhao

Article

Schultz et al Biotechnol Bioeng. 2019 Apr 30. doi: 10.

Insert

SpCas9-NLS3

Use

CRISPR

Expression

Yeast

Promoter

pPGK1

Available Since

April 1, 2020

Availability

Academic Institutions and Nonprofits only

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Cherry-Fibrillarin-C1

Plasmid

#134539

Purpose

Expresses Cherry-Fibrillarin fusion protein

Depositor

Susan Janicki

Article

Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

Insert

Fibrillarin (FBL Human)

Tags

Cherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pJOE7706.1

Plasmid

#135075

Purpose

A shuttle vector for heterologous HA production with Corynebacterium glutamicum

Depositor

Josef Altenbuchner

Article

Hoffmann et al J Appl Microbiol. 2014 Sep;117(3):663-78

Type

Empty backbone

Expression

Bacterial

Available Since

Feb. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pTfR-mNeonGreen

Plasmid

#129608

Purpose

In vivo visualization of Transferrin receptors (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

Transferrin

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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