We narrowed to 4,995 results for: mcs

1. MSCV-N BFLF2

   Plasmid

   #37931

   Depositor

   Karl Munger

   Article

   Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

   Insert

   BFLF2 (BFLF2 )
   

   Use

   Retroviral

   Tags

   Flag and HA

   Expression

   Mammalian

   Promoter

   PKG

   Available Since

   Aug. 1, 2012

   Availability

   Academic Institutions and Nonprofits only

2. MSCV-C 6bE6

   Plasmid

   #37916

   Depositor

   Karl Munger

   Article

   Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

   Insert

   HPV 6b E6 (E6 HPV)
   

   Use

   Retroviral

   Tags

   Flag and HA

   Expression

   Mammalian

   Promoter

   PKG

   Available Since

   July 27, 2012

   Availability

   Academic Institutions and Nonprofits only

3. AAV-GfaABC1D-Cre-4x6T

   Plasmid

   #196410

   Purpose

   Astrocytic expression of Cre recombinase in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette

   Depositor

   Stanley Thomas Carmichael

   Article

   Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

   Has Service

   AAV5

   Insert

   Cre
   

   Use

   AAV; Astrocyte-selective

   Expression

   Mammalian

   Promoter

   CAG and GfaABC1D

   Available Since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMK444 (EF1-OsTIR1(F74G) Puro)

   Plasmid

   #214374

   Purpose

   EF1-OsTIR1(F74G) (PiggyBac)

   Depositor

   Masato Kanemaki

   Article

   Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

   Insert

   EF1-OsTIR1(F74G)
   

   Expression

   Mammalian

   Promoter

   EF1 promoter

   Available Since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. CaMKIIP-mEGFP-P2A-paAIP2/pAAV

   Plasmid

   #91718

   Purpose

   AAV-mediated expression of mEGFP and paAIP2 for optogenetic control of CaMKII

   Depositor

   Ryohei Yasuda

   Article

   Murakoshi et al Neuron. 2017 Apr 5;94(1):37-47.e5. doi:

   Insert

   mEGFP-P2A-paAIP2
   

   Use

   AAV

   Promoter

   CaMKII 1.3

   Available Since

   June 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV CAGG eGFP

   Plasmid

   #107707

   Purpose

   express eGFP in variety of cell types

   Depositor

   Troy Margrie

   Article

   Velez-Fort et al Neuron. 2018 Apr 4;98(1):179-191.e6. doi

   Insert

   eGFP
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CAGG

   Available Since

   Sept. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYC-C

   Plasmid

   #207114

   Purpose

   HDR donor template with Puromycin resistance and mNeonGreen marker flanked by two multiple cloning sites (MCS) for insertion of left and right homology arms (L- and R-arms) for the targeted locus

   Depositor

   Yifan Cheng

   Article

   Choi et al Proc Natl Acad Sci U S A. 2023 Aug;120(3

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Nov. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYC-N

   Plasmid

   #207113

   Purpose

   HDR donor template with Puromycin resistance and mNeonGreen marker flanked by two multiple cloning sites (MCS) for insertion of left and right homology arms (L- and R-arms) for the targeted locus

   Depositor

   Yifan Cheng

   Article

   Choi et al Proc Natl Acad Sci U S A. 2023 Aug;120(3

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Oct. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMS114

   Plasmid

   #215677

   Purpose

   Empty repair plasmid for ChrI split hygromycinR landing pad

   Depositor

   Patrick Phillips

   Article

   Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

   Insert

   5'HA + MCS + loxN + rps-0p::5'ΔHygR
   

   Use

   CRISPR and Cre/Lox

   Expression

   Worm

   Mutation

   5' ∆HYGR encodes aa1-226

   Available Since

   March 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMS158

    Plasmid

    #215679

    Purpose

    Empty repair plasmid for ChrIII split hygromycinR landing pad

    Depositor

    Patrick Phillips

    Article

    Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

    Insert

    5'HA + MCS + lox2272 + rps-0p::5'ΔHygR
    

    Use

    CRISPR and Cre/Lox

    Expression

    Worm

    Mutation

    5' ∆HYGR encodes aa1-226

    Available Since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV2-DEST(r)

    Plasmid

    #190238

    Purpose

    Gateway destination vector, empty AAV vector backbone

    Depositor

    Hiroyuki Hioki

    Article

    Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

    Type

    Empty backbone

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-TRE-EGFP

    Plasmid

    #89875

    Purpose

    EGFP reporter vector of iTango system for AAV production

    Depositor

    Hyungbae Kwon

    Article

    Lee et al Nat Methods. 2017 May;14(5):495-503. doi

    Insert

    tetO-EGFP
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    tetO

    Available Since

    May 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBI-p53 R175H/EGFP

    Plasmid

    #16544

    Depositor

    Bert Vogelstein

    Article

    Yu et al Proc Natl Acad Sci U S A. 1999 Dec 7. 96

    Insert

    p53 (TP53 Human)
    

    Expression

    Mammalian

    Mutation

    R175H

    Available Since

    June 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLentiRhoA2G

    Plasmid

    #40179

    Depositor

    Olivier Pertz

    Article

    Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

    Insert

    RhoA Biosensor
    

    Use

    Lentiviral

    Tags

    His-Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. Stat3 pcDNA3

    Plasmid

    #8706

    Purpose

    See Depositor Comments below for variant information.

    Depositor

    Jim Darnell

    Article

    Zhong et al Proc Natl Acad Sci U S A 1994 May 24;91(

    Insert

    Stat3 (Stat3 Mouse)
    

    Expression

    Mammalian

    Mutation

    * see comments

    Available Since

    Sept. 26, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFH2.32_LV-mCherry-MS2x8-BCS

    Plasmid

    #205539

    Purpose

    Express mCherry-MS2x8 with barcode

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    mCherry
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    EF1

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-Cdc42-2G

    Plasmid

    #68813

    Purpose

    FRET-based biosensor reporting on endogenous Cdc42 activation (for Lentivirus production)

    Depositor

    Olivier Pertz

    Article

    Martin et al Sci Rep. 2016 Feb 25;6:21901. doi: 10.10

    Insert

    Cdc42 activation reporter
    

    Use

    Lentiviral

    Tags

    His and Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHAGE-GFP

    Plasmid

    #106281

    Purpose

    A version of the pHAGE vector with ZsGreen dsRed expressed separated by an IRES. Note that there is little-to-no expression of dsRed.

    Depositor

    Jay Shendure

    Article

    Hill et al Nat Methods. 2018 Feb 19. pii: nmeth.460

    Insert

    CMV-GFP-IRES-DSRED(NOT-EXPRESSED)-WPRE-MCS
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pME-Cas9

    Plasmid

    #63154

    Purpose

    Middle Entry clone for Gateway containing a zebrafish codon-optimized Cas9 flanked by 2 NLS

    Depositor

    Leonard Zon

    Article

    Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15

    Insert

    Cas9
    

    Use

    CRISPR; Tol2 middle entry vector for gateway

    Available Since

    April 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV2-DEST(f)

    Plasmid

    #190226

    Purpose

    Gateway destination vector, empty AAV vector backbone

    Depositor

    Hiroyuki Hioki

    Article

    Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

    Type

    Empty backbone

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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