We narrowed to 10,318 results for: plasmids 101

1. pSIN4-EF2-O2S

   Plasmid

   #21162

   Depositor

   James Thomson

   Article

   Yu et al Science. 2009 Mar 26.

   Insert

   Oct4, Sox2 (SOX2, POU5F1 Human)
   

   Use

   Lentiviral; Reprogramming

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 9, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV.PGK.mFoxa2

   Plasmid

   #33014

   Depositor

   Malin Parmar

   Article

   Pfisterer et al Proc Natl Acad Sci U S A. 2011 Jun 21;10

   Insert

   Forkhead box A2 (Foxa2 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   H10L

   Promoter

   Available Since

   Jan. 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. scFv-GCN4-DNMT3a(R887E)-DNMT3l

   Plasmid

   #154141

   Purpose

   Expresses a higher specificity variant of scFv-GCN4-DNMT3a-DNMT3l, contains R887E mutation in DNMT3a. To be used with the dCas9-SunTag system for targeted DNA methylation.

   Depositor

   Albert Jeltsch

   Article

   Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i

   Insert

   scFv-GCN4, DNMT3a (catalytic domain), DNMT3l (C-terminal part), sfGFP
   

   Use

   Tags

   HA and sfGFP

   Expression

   Mammalian

   Mutation

   R887E

   Promoter

   SFFV

   Available Since

   Oct. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCEP4-FLAG-CCR5

   Plasmid

   #98949

   Purpose

   Mammalian expression plasmid for N-terminal FLAG-tagged human CCR5

   Depositor

   Erik Procko

   Article

   Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

   Insert

   CCR5 (CCR5 Human)
   

   Use

   Tags

   FLAG (dykdddd) epitope tag and Signal/leader sequ…

   Expression

   Mammalian

   Mutation

   Full-length, wildtype

   Promoter

   CMV

   Available Since

   April 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEMS2232

   Plasmid

   #158401

   Purpose

   High Copy number plasmid containing Ple316 (NR2E1 MiniPromoter) insert

   Depositor

   Elizabeth Simpson

   Article

   Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

   Insert

   Ple316 (NR2E1 Human)
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Ple316

   Available Since

   Feb. 4, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pCEP4-HA-CCR5

   Plasmid

   #98950

   Purpose

   Mammalian expression plasmid for N-terminal HA-tagged human CCR5

   Depositor

   Erik Procko

   Article

   Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

   Insert

   CCR5 (CCR5 Human)
   

   Use

   Tags

   HA (YPYDVPDYA) epitope tag and Signal/leader sequ…

   Expression

   Mammalian

   Mutation

   Full-length, wildtype

   Promoter

   CMV

   Available Since

   April 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCEP4-myc-CCR5

   Plasmid

   #98948

   Purpose

   Mammalian expression plasmid for N-terminal myc-tagged human CCR5

   Depositor

   Erik Procko

   Article

   Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

   Insert

   CCR5 (CCR5 Human)
   

   Use

   Tags

   Signal/leader sequence from Influenza A virus HA …

   Expression

   Mammalian

   Mutation

   Full-length, wildtype

   Promoter

   CMV

   Available Since

   April 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. ATP9A-luciferase

   Plasmid

   #113354

   Purpose

   evaluate FOXA1 DIV motif enhancer activity near 50 kb of ATP9A gene

   Depositor

   Ralf Jauch

   Article

   Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

   Insert

   ATP9A-enhancer (ATP9A Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV CAG ChR2 E123T T159C 2A tDimer

   Plasmid

   #85399

   Purpose

   High efficiency channelrhodopsin with cytomegalovirus enhancer fused to chicken β-actin (CAG) promoter

   Depositor

   Thomas Oertner

   Article

   Berndt et al Proc Natl Acad Sci U S A. 2011 May 3;108

   Inserts

   Channelrhodopsin-2
   red fluorescent protein
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   E123T , T159C

   Promoter

   CAG and ribosomal skip sequence 2A

   Available Since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-DYRK4-2A-GFP

    Plasmid

    #118288

    Purpose

    Expresses mouse DYRK4 tagged with 2A peptide at it's C-terminus and GFP in mammalian cells.

    Depositor

    Vance Lemmon

    Article

    Slepak et al Cytoskeleton (Hoboken). 2012 Jul;69(7):5

    Insert

    dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 4 (Dyrk4 Mouse)
    

    Use

    AAV

    Tags

    2A peptide and EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV with beta global intron

    Available Since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-DYRK4-2A-mCherry

    Plasmid

    #118289

    Purpose

    Expresses mouse DYRK4 tagged with 2A peptide at it's C-terminus and mCherry in mammalian cells.

    Depositor

    Vance Lemmon

    Article

    Slepak et al Cytoskeleton (Hoboken). 2012 Jul;69(7):5

    Insert

    dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 4 (Dyrk4 Mouse)
    

    Use

    AAV

    Tags

    2A peptide and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV with beta global intron

    Available Since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV_FLAG_hSF3B1

    Plasmid

    #176590

    Purpose

    Adenoviral construct to insert a N-terminal FLAG to one allele of human SF3B1

    Depositor

    Manoj Pillai

    Article

    Boddu et al Commun Biol. 2021 Oct 13;4(1):1184. doi:

    Insert

    SF3B1 (SF3B1 Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET-Duet1-6xHis-TEV-OPTN

    Plasmid

    #190192

    Purpose

    Plasmid for the expression of 6xHis-TEV-OPTN. Internal reference: SMC397

    Depositor

    Sascha Martens

    Article

    Zaffagnini et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

    Insert

    optineurin (OPTN Human)
    

    Use

    Tags

    6xHis and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-EF1α1.1-FLEX-rc [ChromeQ-GFP]

    Plasmid

    #153542

    Purpose

    AAV-mediated expression of ChromeQ-GFP under the EF1α promoter (1.1kb short version), in floxed/reversed (Cre-dependent) manner.

    Depositor

    Edward Boyden

    Article

    Cho et al J Biol Chem. 2019 Jan 4. pii: RA118.0069

    Insert

    ChromeQ-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α1.1

    Available Since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-EF1α1.1-FLEX-rc [ChromeT-GFP]

    Plasmid

    #153541

    Purpose

    AAV-mediated expression of ChromeT-GFP under the EF1α promoter (1.1kb short version), in floxed/reversed (Cre-dependent) manner.

    Depositor

    Edward Boyden

    Article

    Cho et al J Biol Chem. 2019 Jan 4. pii: RA118.0069

    Insert

    ChromeT-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α1.1

    Available Since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKLV2-U6gRNA5(Pggt1b-g1)-PGKpuroBFP-W

    Plasmid

    #105018

    Purpose

    Lentiviral gRNA plasmid targeting mouse Pggt1b , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Pggt1b (Pggt1b Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKLV2-U6gRNA5(Znrf1-g1)-PGKpuroBFP-W

    Plasmid

    #105020

    Purpose

    Lentiviral gRNA plasmid targeting mouse Znrf1 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Znrf1 (Znrf1 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDDGFP-Leu2d-GgKDELR2 R159K

    Plasmid

    #123626

    Purpose

    Expresses GgKDELR2 with Arg159Lys mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

    Depositor

    Simon Newstead

    Article

    Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

    Insert

    KDELR2 (KDELR2 Chicken)
    

    Use

    Tags

    TEV-GFP-His

    Expression

    Yeast

    Mutation

    Arg159Lys

    Promoter

    Available Since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDDGFP-Leu2d-GgKDELR2 H12A

    Plasmid

    #123619

    Purpose

    Expresses GgKDELR2 with His12Ala mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

    Depositor

    Simon Newstead

    Article

    Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

    Insert

    KDELR2 (KDELR2 Chicken)
    

    Use

    Tags

    TEV-GFP-His

    Expression

    Yeast

    Mutation

    His12Ala

    Promoter

    Available Since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDDGFP-Leu2d-GgKDELR2 R47A

    Plasmid

    #123620

    Purpose

    Expresses GgKDELR2 with Arg47Ala mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

    Depositor

    Simon Newstead

    Article

    Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

    Insert

    KDELR2 (KDELR2 Chicken)
    

    Use

    Tags

    TEV-GFP-His

    Expression

    Yeast

    Mutation

    Arg47Ala

    Promoter

    Available Since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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