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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 70,757 results for: LAS

Showing: 5911 - 5940 of 70757 results

  1. pQE30del

    Plasmid
    #106902
    Purpose
    pQE30del is conditionally replicating empty vector. Replication of the plasmid is inhibited in presense of IPTG or lactose. pQE30del can be used for construction of any E. coli helper plasmids.
    Depositor
    Sergey Sineoky
    Article
    Bubnov et al J Microbiol Methods. 2018 Jun 7. pii: S0
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    106902-plasmid-map-sequence-id-327626

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  4. KLHL3 (KLHL3A-c003)

    Plasmid
    #110251
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    KLHL3 (KLHL3 Human)
    Use
    Tags
    His-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7 - lacO
    Available since
    June 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    110251-plasmid-map-sequence-id-212338

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  6. SARS-CoV-2 Spike-d18

    Plasmid
    #164583
    Purpose
    A mammalian expression vector to express codon-optimized SARS-CoV-2 Spike protein, with a C terminal truncation of 18 amino acids to improve pseudoparticle generation.
    Depositor
    Jason Sheltzer
    Insert
    SARS-CoV-2 Spike (S SARS-CoV-2)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Deleted amino acids 1256-1273
    Promoter
    EF1a
    Available since
    Jan. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    164583-plasmid-map-sequence-id-322362

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  7. KEAP1 (KEAP1A-c029)

    Plasmid
    #110247
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    KEAP1 (KEAP1 Human)
    Use
    Tags
    His-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7 - lacO
    Available since
    June 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    110247-plasmid-map-sequence-id-212283

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  10. pAAV-CMV-FLEX-SaCas9-U6-sgKcnd3

    Plasmid
    #159912
    Purpose
    Mutagenesis of Kcnd3
    Depositor
    Larry Zweifel
    Insert
    Kcnd3 (Kcnd3 Mouse)
    Use
    AAV, CRISPR, and Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    159912-plasmid-map-sequence-id-317319

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  14. ACE2 g1 + Cas9

    Plasmid
    #153011
    Purpose
    A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2
    Depositor
    Jason Sheltzer
    Insert
    Cas9 + ACE2 gRNA (ACE2 Human)
    Use
    CRISPR and Lentiviral
    Tags
    TagBFP2
    Expression
    Mutation
    Promoter
    Available since
    June 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    153011-plasmid-map-sequence-id-298673

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  16. pAAV Syn 2xLyn-ERex-mKate2-bPAC(F198Y)

    Plasmid
    #219654
    Purpose
    Neuronal expression of PACmn with mKate2, a membrane targeted version of the photoactivatable adenylyl cyclase from Beggiatoa with lowered dark activity.
    Depositor
    Thomas Oertner
    Insert
    2xLyn-ERex-mKate2-bPAC(F198Y)
    Use
    AAV
    Tags
    ER exit ( FCYENE) and Lyn11 (GCIKSKGKDS)
    Expression
    Mammalian
    Mutation
    Promoter
    Syn
    Available since
    May 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    219654-plasmid-map-sequence-id-434848

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  18. pAAV-Ef1a-DIO-Synaptophysin-GCaMP6s

    Plasmid
    #105715
    Purpose
    In the presence of Cre, the plasmid can be used to express GCaMP6s fused to the presynaptic protein synaptophysin. Drives enriched expression of GCaMP6s in neuronal axon boutons.
    Depositor
    Rylan Larsen
    Has Service
    AAV5
    Insert
    synaptophysin-GCaMP6s
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Ef1a, Human elongation factor-1 alpha
    Available since
    April 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    105715-plasmid-map-sequence-id-207274

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  19. ANO10 (TMEM16KA-c001)

    Plasmid
    #110267
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    TMEM16K (ANO10 Human)
    Use
    Tags
    TEV-His10-Flag
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin promoter
    Available since
    April 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    110267-plasmid-map-sequence-id-358733

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  20. pELK3.1.0-gDNA

    Plasmid
    #112418
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ELK3
    Depositor
    Kevin White
    Insert
    ELK3 (ELK3 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112418-plasmid-map-sequence-id-230297

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  21. pATF4.1.0-gDNA

    Plasmid
    #113772
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ATF4
    Depositor
    Kevin White
    Insert
    ATF4 (ATF4 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    113772-plasmid-map-sequence-id-230558

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  24. JMJD1CA-c089

    Plasmid
    #79644
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    JMJD1C (JMJD1C Human)
    Use
    Tags
    6x His and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    79644-plasmid-map-sequence-id-150204

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  25. pJUNB.1.0-gDNA

    Plasmid
    #112409
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor JUNB
    Depositor
    Kevin White
    Insert
    JUNB (JUNB Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112409-plasmid-map-sequence-id-230268

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  26. ACE2 g2

    Plasmid
    #153012
    Purpose
    A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing mCherry
    Depositor
    Jason Sheltzer
    Insert
    ACE2 gRNA (ACE2 Human)
    Use
    CRISPR and Lentiviral
    Tags
    mCherry
    Expression
    Mutation
    Promoter
    Available since
    June 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    153012-plasmid-map-sequence-id-298678

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  27. pJYDNgp

    Plasmid
    #162453
    Purpose
    Positive control plasmid for pJYDNg carrying the eUnaG2 bilirubin dependent fluorescent protein fused to the C-terminus of Aga2p protein. This plasmid contains a long 2G linker.
    Depositor
    Gideon Schreiber
    Article
    Zahradník et al bioRxiv Preprint 2020
    Insert
    Aga2p-2Glinker-eUnaG2 (AGA2 )
    Use
    Shuttle vector bacteria/yeast
    Tags
    HA nad myc and eUnaG2 fluorescent protein
    Expression
    Mutation
    Promoter
    GAL1
    Available since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    162453-plasmid-map-sequence-id-321460

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  28. ACE2 g4 + Cas9

    Plasmid
    #153014
    Purpose
    A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2
    Depositor
    Jason Sheltzer
    Insert
    Cas9 + ACE2 gRNA (ACE2 Human)
    Use
    Lentiviral
    Tags
    TagBFP2
    Expression
    Mutation
    Promoter
    Available since
    June 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    153014-plasmid-map-sequence-id-298677

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Showing: 5911 - 5940 of 70757 results