We narrowed to 7,281 results for: 11

1. MLF2-GFP-3xFLAG

   Plasmid

   #230986

   Purpose

   Expresses GFP tagged MLF2 in mammalian cells

   Depositor

   Christian Schlieker

   Article

   Prophet et al Nat Cell Biol. 2022 Nov;24(11):1630-1641

   Insert

   MLF2 (MLF2 Human)
   

   Tags

   3xFLAG and GFP

   Expression

   Mammalian

   Promoter

   T7

   Available Since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGL4 mIL-17 2kb RORgamma-t mut

   Plasmid

   #20127

   Depositor

   Warren Strober

   Article

   Fuping et al 2008 Nov;9(11):1297-306. Epub 2008 Oct 1

   Insert

   mIL-17 (Il17a Mouse)
   

   Use

   Luciferase

   Mutation

   RORgamma-t binding site in 0.9kb of 2kb promoter …

   Available Since

   Jan. 16, 2009

   Availability

   Academic Institutions and Nonprofits only

3. pAS_4xMmaPylT(UCA)_EF1_MmaPylRS

   Plasmid

   #174899

   Purpose

   Mma PylRS expression for opal suppression in mammalian cells; transient or piggy bac mediated integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

   Insert

   Mma PylRS (pylS Methanosarcina mazei)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   EF1

   Available Since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. VRQR variant

   Plasmid

   #138562

   Purpose

   Expresses human codon-optimized SpCas9-VRQR and blasticidin resistance: EFS promoter-VRQR-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

   Insert

   VRQR
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   D1135V, G1218R, R1335Q, T1337R

   Promoter

   EFS

   Available Since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pB-CAG-FLEX-F14F15-BFP-(mCherry)'

   Plasmid

   #201831

   Purpose

   piggyBac transposon plasmid for making Cre and Flpo reporter cell lines using piggyBac transposase

   Depositor

   Ian Wickersham

   Article

   Jin et al Cell Rep Methods. 2023 Nov 20;3(11):1006

   Inserts

   CAG
   BFP
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   April 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKD068

   Plasmid

   #136471

   Purpose

   SSB-GFP IDL fusion expression plasmid

   Depositor

   James Keck

   Article

   Dubiel et al Nucleic Acids Res. 2020 Jun 19;48(11):60

   Insert

   SSB-GFP (ssb E. coli)
   

   Tags

   superfolder GFP

   Expression

   Bacterial

   Mutation

   superfolder GFP inserted between Phe148 and Ser149

   Promoter

   T7 promoter

   Available Since

   April 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCfB2904(XI-3 MarkerFree)

   Plasmid

   #73276

   Purpose

   EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-3 (Chr XI: 93378..94567)

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Yeast

   Available Since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. GB1-A11(89-196)

   Plasmid

   #196215

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(89-196).

   Depositor

   Lalit Deshmukh

   Article

   Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

   Insert

   Annexin A11 (ANXA11 Human)
   

   Tags

   GB1-6xHis-TEV and Strep-II tag

   Expression

   Bacterial

   Available Since

   July 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. GB1-A11(2-68)-Strep

   Plasmid

   #196216

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-68)-Strep.

   Depositor

   Lalit Deshmukh

   Article

   Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

   Insert

   Annexin A11 (ANXA11 Human)
   

   Tags

   GB1-6xHis-TEV and Strep-II tag

   Expression

   Bacterial

   Available Since

   July 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. GB1-A11(2-52)-Strep

    Plasmid

    #196213

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-52)-Strep.

    Depositor

    Lalit Deshmukh

    Article

    Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

    Insert

    Annexin A11 (ANXA11 Human)
    

    Tags

    GB1-6xHis-TEV and Strep-II tag

    Expression

    Bacterial

    Available Since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET21-pelB-LaM-3

    Plasmid

    #172769

    Purpose

    Bacterial expression of anti-mCherry nanobody LaM-3, with pelB leader and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaM-3
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. RSV/hGATA3

    Plasmid

    #83814

    Purpose

    Mammalian expression of human GATA3

    Depositor

    Douglas Engel

    Article

    Ko et al Mol Cell Biol. 1991 May . 11(5):2778-84.

    Insert

    GATA3 (GATA3 Human)
    

    Expression

    Mammalian

    Promoter

    RSV

    Available Since

    Oct. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCfB2909(XII-5 MarkerFree)

    Plasmid

    #73281

    Purpose

    EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-5 (Chr XII: 839226..840357)

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDNA3 Flag Jnk2a2(apf)

    Plasmid

    #13761

    Depositor

    Roger Davis

    Article

    Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

    Insert

    Jnk2a2(apf) (MAPK9 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    replaced dual phosporylation motif THr(183)-Pro-T…

    Available Since

    Feb. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET21-pelB-LaM-8

    Plasmid

    #172772

    Purpose

    Bacterial expression of anti-mCherry nanobody LaM-8, with pelB leader and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaM-8
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-SNAP-CRY2-VHH(GFP)

    Plasmid

    #58370

    Purpose

    For use in light-induced inactivation of GFP fused protein through the interaction with CIB1-MP

    Depositor

    Won Do Heo

    Article

    Lee et al Nat Methods. 2014 Jun;11(6):633-6. doi:

    Insert

    CRY2[1-498] (CRY2 Mustard Weed)
    

    Tags

    SNAP and vhhGFP4

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET21-pelB-LaG-30

    Plasmid

    #172754

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-30; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-30
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET21-pelB-LaM-2

    Plasmid

    #172768

    Purpose

    Bacterial expression of anti-mCherry nanobody LaM-2, with pelB leader and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaM-2
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-CBX1mut-PA-mCherry

    Plasmid

    #138251

    Purpose

    Triple-mutant CBX1 chromodomain (residues 20-73) tagged with photoactivatable mCherry (PAmCherry). Mutations V3E/K6E/D40S correspond to positions V22/K25/D59 in full-length CBX1.

    Depositor

    Jin Zhang

    Article

    Hard et al Sci Adv. 2018 Nov 7;4(11):eaau1447. doi:

    Insert

    CBX1mut-PAmCherry
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCRI006-pYFAC-riboB-PgpdA-dSpCas9-VPR-TtrpC

    Plasmid

    #140199

    Purpose

    Episomal expression of dSpCas9-VPR. Filamentous fungi vector with AMA1 and riboB selection marker.

    Depositor

    Yit Heng Chooi

    Article

    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

    Insert

    dSpCas9-VPR
    

    Use

    CRISPR and Synthetic Biology; Expression in asper…

    Tags

    VPR (VP64-p65-Rta), NLS

    Promoter

    PgpdA

    Available Since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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