We narrowed to 4,214 results for: Abo;

1. Ydf NanoLuc

   Plasmid

   #225656

   Purpose

   Reporter plasmid expressing NanoLuciferase under the control of the promoter PydfJ115, cognate to YdfI. Responds to NarL/NarX chimeras from this study.

   Depositor

   Neha Kamat

   Article

   Peruzzi et al Proc Natl Acad Sci U S A. 2023 May 9;120

   Insert

   NanoLuciferase
   

   Use

   Cell free expression

   Tags

   Myc

   Expression

   Bacterial

   Mutation

   Promoter

   PydfJ115

   Available since

   Dec. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJL NarX Myc

   Plasmid

   #225651

   Purpose

   Expresses WT NarX capable of sensing nitrate. For use with NarL-YdfI and reporter plasmids from this study.

   Depositor

   Neha Kamat

   Article

   Peruzzi et al Proc Natl Acad Sci U S A. 2023 May 9;120

   Insert

   NarX (narX E. coli)
   

   Use

   Cell free expression

   Tags

   Myc

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pUC-hU6-2O-crScaffold_SV40-BFP

   Plasmid

   #224861

   Purpose

   crRNA scaffold for RfxCas13d expressed from hU6-2xTetO promoter and reporter BFP protein expressed from SV40 promoter

   Depositor

   Gabor Balazsi

   Article

   Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6-2xTetO

   Available since

   Oct. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pUC-hU6-2O-crScaffold_EF1a-BFP

   Plasmid

   #224862

   Purpose

   crRNA scaffold for RfxCas13d expressed from hU6-2xTetO promoter and reporter BFP protein expressed from EF1a promoter

   Depositor

   Gabor Balazsi

   Article

   Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6-2xTetO

   Available since

   Oct. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. LPUtopia-8

   Plasmid

   #224781

   Purpose

   Landing Pad donor vector for AAVS1 site integration in human and VeroE6 cells

   Depositor

   Gabor Balazsi

   Article

   Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

   Inserts

   puromycin N-acetyltransferase
   enhanced Green Fluorescence Protein
   Red Fluorescence Protein
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pUC-ODN-hsRPL3-CterTwStrep-ΔPAM62&56

   Plasmid

   #208645

   Purpose

   Remove PAM region in HomoArms by introduce SNP rs7495557 and rs760194863, to prevent cleavage after success edit. Digest with nt.BspQI and nb.BsrDI to get ssDNA.

   Depositor

   Quanfu Ma

   Insert

   Twin Strep tag with RPL3 genome HomoArm (RPL3 Human, Synthetic)
   

   Use

   CRISPR; Ssdna donnor preparation for knock-in of…

   Tags

   Twin Strep

   Expression

   Mutation

   Two SNP (rs749555789, rs760194863) were introduce…

   Promoter

   Available since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pQE60 G121A wgMDH

   Plasmid

   #204252

   Purpose

   Bacterial expression of G121A wgMDH with IPTG induction - Active site loop mutant

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Article

   Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

   Insert

   MDHG_CITLA
   

   Use

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   G121A

   Promoter

   Available since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pQE60 R196A wgMDH

   Plasmid

   #204271

   Purpose

   Bacterial expression of R196A wgMDH with IPTG induction - Active site mutant

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Article

   Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

   Insert

   MDHG_CITLA
   

   Use

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   R196A

   Promoter

   Available since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pQE60 R196E wgMDH

   Plasmid

   #204272

   Purpose

   Bacterial expression of R196E wgMDH with IPTG induction - Active site mutant

   Depositor

   Malate dehydrogenase CUREs Community (MCC)

   Article

   Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

   Insert

   MDHG_CITLA
   

   Use

   Tags

   6X His

   Expression

   Bacterial

   Mutation

   R196E

   Promoter

   Available since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pQE60 P123A wgMDH

    Plasmid

    #204254

    Purpose

    Bacterial expression of P123A wgMDH with IPTG induction - Active site loop mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    P123A

    Promoter

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pQE60 R130Q wgMDH

    Plasmid

    #204268

    Purpose

    Bacterial expression of R130Q wgMDH with IPTG induction - Active site mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    R130Q

    Promoter

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pQE60 G127F wgMDH

    Plasmid

    #204264

    Purpose

    Bacterial expression of G127F wgMDH with IPTG induction - Active site loop mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    G127F

    Promoter

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pQE60 P126R wgMDH

    Plasmid

    #204261

    Purpose

    Bacterial expression of P126R wgMDH with IPTG induction - Active site loop mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    P126R

    Promoter

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pQE60 G127A wgMDH

    Plasmid

    #204263

    Purpose

    Bacterial expression of G127A wgMDH with IPTG induction - Active site loop mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    G127A

    Promoter

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pQE60 M66L wgMDH

    Plasmid

    #204247

    Purpose

    Bacterial expression of M66L wgMDH with IPTG induction - Subunit interface mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    M66L

    Promoter

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pQE60 G127P wgMDH

    Plasmid

    #204265

    Purpose

    Bacterial expression of G127P wgMDH with IPTG induction - Active site loop mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    G127P

    Promoter

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pQE60 G121F wgMDH

    Plasmid

    #204253

    Purpose

    Bacterial expression of G121F wgMDH with IPTG induction - Active site loop mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    G121F

    Promoter

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pQE60 M91Q wgMDH

    Plasmid

    #204250

    Purpose

    Bacterial expression of M91Q wgMDH with IPTG induction - Subunit interface mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    M91Q

    Promoter

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pQE60 D87L wgMDH

    Plasmid

    #204248

    Purpose

    Bacterial expression of D87L wgMDH with IPTG induction - Subunit interface mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    D87L

    Promoter

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pQE60 P126G wgMDH

    Plasmid

    #204260

    Purpose

    Bacterial expression of P126G wgMDH with IPTG induction - Active site loop mutant

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Article

    Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

    Insert

    MDHG_CITLA
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    P126G

    Promoter

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge