We narrowed to 10,320 results for: plasmids 101

1. Gal4-TET1-878-911-L897A(pL84)

   Plasmid

   #124401

   Purpose

   Gal4/VP16 luciferase reporter assay. Gal4 DBD fusion of TET1 SID with L897A mutation.

   Depositor

   Shaun Cowley

   Article

   Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

   Insert

   Gal4-TET1-878-911(L897A mutation) (TET1 Human)
   

   Use

   Tags

   gal4 dna binding domain

   Expression

   Mammalian

   Mutation

   L897A

   Promoter

   Available Since

   May 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-CMV-LNI-bGH

   Plasmid

   #233203

   Purpose

   AAV expression of Lhx3, Ngn2x3HA, and Isl1 for motor neuron reprogramming

   Depositor

   Kate Galloway

   Article

   Wang et al Cell Syst. 2025 Mar 10:101206. doi: 10.1

   Insert

   mLhx3-P2A-mNgn2x3HA-T2A-Isl1 (Neurog2, Lhx3, Isl1 Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-CAG-SNAP-p53DD-IRES-HRAS(G12V)-bGH

   Plasmid

   #233204

   Purpose

   AAV expression of SNAP-p53DD and HRAS(G12V)

   Depositor

   Kate Galloway

   Article

   Wang et al Cell Syst. 2025 Mar 10:101206. doi: 10.1

   Insert

   SNAP-p53DD-IRES-HRAS(G12V) (Trp53, HRAS Human, Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   G12V

   Promoter

   Available Since

   March 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-T7-TadCBEd-SpRY-P2A-EGFP (BKS428)

   Plasmid

   #223125

   Purpose

   pCMV and pT7 Human expression plasmid for TadCBEd-SpRY with C-term BPNLS-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized TadCBEd-SpRY-2xUGI-P2A-EGFP
   

   Use

   CRISPR

   Tags

   2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

   Expression

   Mammalian

   Mutation

   TadA-CDd mutations; nSpRY=D10A/A61R/L1111R/D1135L…

   Promoter

   CMV and T7

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLY080b_AAV_EFS-4D5-CD8-28BBz (HER2 CAR)

   Plasmid

   #192190

   Purpose

   HER2 CAR AAV vector PRODH2 KI (pLY080b)

   Depositor

   Sidi Chen

   Article

   Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

   Insert

   HER2 CAR AAV vector PRODH2 KI (pLY080b) (ERBB2 Synthetic)
   

   Use

   AAV; Mammalian expression

   Tags

   Expression

   Mutation

   NA

   Promoter

   Available Since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-T7-ABE8e-SpCas9(LWKYQS)-P2A-EGFP (AHK404)

   Plasmid

   #223113

   Purpose

   pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with LWKYQS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized TadA8e-SpCas9(LWKYQS)-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS-P2A-EGFP and BPNLS-TadA8e

   Expression

   Mammalian

   Mutation

   TadA8e mutations; nSpCas9(D10A/LWKYQS)=D1135L, S1…

   Promoter

   CMV and T7

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV-T7-ABE8e-SpCas9(LWRYEK)-P2A-EGFP (BKS1028)

   Plasmid

   #223114

   Purpose

   pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with LWRYEK amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized TadA8e-SpCas9(LWRYEK)-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS-P2A-EGFP and BPNLS-TadA8e

   Expression

   Mammalian

   Mutation

   TadA8e mutations; nSpCas9(D10A/LWRYEK)=D1135L, S1…

   Promoter

   CMV and T7

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTwist-SARS-CoV-2 Δ18 B.1.617.2v1

   Plasmid

   #179905

   Purpose

   Encodes SARS-CoV-2 B.1.1.529 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Garcia-Beltran et al Cell. 2022 Jan 6. pii: S0092-8674(21)014

   Insert

   SARS-CoV-2 B.1.617.2v1 Spike truncated to remove 18 amino acids (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   T19R; G142D; E156G; Δ157-158; L452R; T478K; D614G…

   Promoter

   CAG

   Available Since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-T7-ABE8e-SpCas9(MKRCMV)-P2A-EGFP (BKS1030)

   Plasmid

   #223115

   Purpose

   pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with MKRCMV amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized TadA8e-SpCas9(MKRCMV)-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS-P2A-EGFP and BPNLS-TadA8e

   Expression

   Mammalian

   Mutation

   TadA8e mutations; nSpCas9(D10A/MKRCMV)=D1135M, S1…

   Promoter

   CMV and T7

   Available Since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV-T7-ABE8e-SpCas9(MKRCKS)-P2A-EGFP (RAS3455)

    Plasmid

    #223116

    Purpose

    pCMV and pT7 Human expression plasmid for ABE8e using SpCas9 enzyme with MKRCKS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadA8e-SpCas9(MKRCKS)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    BPNLS-P2A-EGFP and BPNLS-TadA8e

    Expression

    Mammalian

    Mutation

    TadA8e mutations; nSpCas9(D10A/MKRCKS)=D1135M, S1…

    Promoter

    CMV and T7

    Available Since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Spike Display_B.1.1.7

    Plasmid

    #172735

    Purpose

    Expression vector for SARS-CoV-2 (B.1.1.7) spike used in the Spike Display system

    Depositor

    IIya Finkelstein

    Article

    Javanmardi et al Mol Cell. 2021 Dec 16;81(24):5099-5111.e

    Insert

    SARS-CoV-2 S B.1.1.7 (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…

    Expression

    Mammalian

    Mutation

    Ectodomain only (AAs 1-1208); 682-685 (furin site…

    Promoter

    CMV

    Available Since

    Aug. 17, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. Spike Display_B.1.1.248

    Plasmid

    #172737

    Purpose

    Expression vector for SARS-CoV-2 (B.1.1.248) spike used in the Spike Display system

    Depositor

    IIya Finkelstein

    Article

    Javanmardi et al Mol Cell. 2021 Dec 16;81(24):5099-5111.e

    Insert

    SARS-CoV-2 S B.1.1.248 (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…

    Expression

    Mammalian

    Mutation

    Ectodomain only (AAs 1-1208); 682-685 (furin site…

    Promoter

    CMV

    Available Since

    Aug. 18, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. Spike Display_B.1.351

    Plasmid

    #172736

    Purpose

    Expression vector for SARS-CoV-2 (B.1.351) spike used in the Spike Display system

    Depositor

    IIya Finkelstein

    Article

    Javanmardi et al Mol Cell. 2021 Dec 16;81(24):5099-5111.e

    Insert

    SARS-CoV-2 S B.1.351 (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…

    Expression

    Mammalian

    Mutation

    Ectodomain only (AAs 1-1208); 682-685 (furin site…

    Promoter

    CMV

    Available Since

    Aug. 18, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. Spike Display_B.1.427/B.1.429

    Plasmid

    #172738

    Purpose

    Expression vector for SARS-CoV-2 (B.1.427/B.1.429) spike used in the Spike Display system

    Depositor

    IIya Finkelstein

    Article

    Javanmardi et al Mol Cell. 2021 Dec 16;81(24):5099-5111.e

    Insert

    SARS-CoV-2 S B.1.427/B.1.429 (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…

    Expression

    Mammalian

    Mutation

    Ectodomain only (AAs 1-1208); 682-685 (furin site…

    Promoter

    CMV

    Available Since

    Aug. 8, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pLIB_3xFlag-His-nsp5CS-nsp9 (SARS-CoV-2)

    Plasmid

    #169177

    Purpose

    Baculoviral transfer vector to express SARS-CoV-2 nsp9 with nsp5-cleavable 3xFlag-His6 tag in insect cells

    Depositor

    John Diffley

    Article

    Milligan et al Biochem J. 2021 Jul 16;478(13):2499-2515

    Insert

    3xFlag-His-nsp5CS-nsp9 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    3xFlag-His6 (nsp5-cleavable)

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDMJ2-SARS-CoV-2-Spike (XBB)

    Plasmid

    #241118

    Purpose

    Expresses SARS-CoV-2 spike (XBB) protein for creating pseudotyped virus

    Depositor

    Jeremy Luban

    Article

    Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

    Insert

    Spike (XBB) (S SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T19I,L24del,P25del,P26del,A27S,V83A,G142D,Y144del…

    Promoter

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDMJ2-SARS-CoV-2-Spike (BQ.1.1)

    Plasmid

    #241111

    Purpose

    Expresses SARS-CoV-2 spike (BQ.1.1) protein for creating pseudotyped virus

    Depositor

    Jeremy Luban

    Article

    Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

    Insert

    Spike (BQ.1.1) (S SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T19I,L24del,P25del,P26del,A27S,H69del,V70del,G142…

    Promoter

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDMJ2-SARS-CoV-2-Spike (BQ.1)

    Plasmid

    #241110

    Purpose

    Expresses SARS-CoV-2 spike (BQ.1) protein for creating pseudotyped virus

    Depositor

    Jeremy Luban

    Article

    Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

    Insert

    Spike (BQ.1) (S SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T19I,L24del,P25del,P26del,A27S,H69del,V70del,G142…

    Promoter

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDMJ2-SARS-CoV-2-Spike (BA.5-2f)

    Plasmid

    #241103

    Purpose

    Expresses SARS-CoV-2 spike (BA.5-2f) protein for creating pseudotyped virus

    Depositor

    Jeremy Luban

    Article

    Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

    Insert

    Spike (BA.5-2f) (S SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T76I,M153S,T19I,L24del,P25del,P26del,A27S,H69del,…

    Promoter

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDMJ2-SARS-CoV-2-Spike (BA.5-2c)

    Plasmid

    #241100

    Purpose

    Expresses SARS-CoV-2 spike (BA.5-2c) protein for creating pseudotyped virus

    Depositor

    Jeremy Luban

    Article

    Youssef et al Immunity. 2025 May 6:S1074-7613(25)00178

    Insert

    Spike (BA.5-2c) (S SARS-CoV-2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Y473F,Q493R,T19I,L24del,P25del,P26del,A27S,H69del…

    Promoter

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge