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Plasmid#162581PurposeExpresses norovirus GI.1 VP1 protein with mutations Q62C-A140C in Sf9 insect cellsDepositorInsertVP1 protein from human norovirus GI.1
UseTagsExpressionInsectMutationGlutamine 62 changed to Cysteine and Alanine 140 ā¦PromoterpolyhedringAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1/MBP-His
Plasmid#38008DepositorInsertMBP (Maltose binding protein)
UseBaculovirus expressionTags6xHISExpressionInsectMutationPromoterPolyhedrinAvailable sinceOct. 1, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFastBac1-NEDD4L_ĪC2 (157)
Plasmid#187720PurposeExpress GST-tagged NEDD4L ĪC2 (157) in insect cellsDepositorInsertNEDD4L residues 157-975 (NEDD4L Human)
UseTagsGST-HRV 3C siteExpressionInsectMutationdeletion of residues 1-156PromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-C17orf53-Strep
Plasmid#211446Purposegeneration of Bacmid for insect cell expression of C17orf53 C-terminally fused to Strep-TagDepositorInsertC17orf53 (HROB Human)
UseTagsStrep-TagExpressionInsectMutationPromoterAvailable sinceMarch 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-C17orf53-FLAG
Plasmid#211447Purposegeneration of Bacmid for insect cell expression of C17orf53 C-terminally fused to FLAG-TagDepositorInsertC17orf53 (HROB Human)
UseTagsFLAG-TagExpressionInsectMutationPromoterAvailable sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-bullfrog saxiphilin
Plasmid#140595Purposeprotein expressionDepositorInsertsaxiphilin bullfrog
UseTagsGFP-His10ExpressionInsectMutationDeletion of M20 - please see depositor commentPromoterpolyhedrin promoterAvailable sinceNov. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1_GI.1_A37C-A44C
Plasmid#162582PurposeExpresses norovirus GI.1 VP1 protein with mutations A37C-A44C in Sf9 insect cellsDepositorInsertVP1 protein from human norovirus GI.1
UseTagsExpressionInsectMutationAlanine 37 changed to Cysteine and Alanine 44 chaā¦PromoterpolyhedringAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1_GI.1_A37I-A44L
Plasmid#162583PurposeExpresses norovirus GI.1 VP1 protein with mutations A37I-A44L in Sf9 insect cellsDepositorInsertVP1 protein from human norovirus GI.1
UseTagsExpressionInsectMutationA37I and A44LPromoterpolyhedrinAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1_GI.1_Q141V-P221L
Plasmid#162584PurposeExpresses norovirus GI.1 VP1 protein with mutations Q141V-P221L in Sf9 insect cellsDepositorInsertVP1 protein from human norovirus GI.1
UseTagsExpressionInsectMutationGlutamine 141 changed to Valine and Proline 221 cā¦PromoterpolyhedrinAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1_GI.1_L144C-P221C
Plasmid#162585PurposeExpresses norovirus GI.1 VP1 protein with mutations L144C-P221C in Sf9 insect cellsDepositorInsertVP1 protein from human norovirus GI.1
UseTagsExpressionInsectMutationLeucine 144 changed to Cysteine and Proline 221 cā¦PromoterpolyhderinAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1_GI.1_G131C-N172C
Plasmid#162586PurposeExpresses norovirus GI.1 VP1 protein with mutations G131C-N172C in Sf9 insect cellsDepositorInsertVP1 protein from human norovirus GI.1
UseTagsExpressionInsectMutationGlycine 131 changed to Cysteine and Asparagine 17ā¦PromoterpolyhedrinAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1_GI.1_N167C-L169C
Plasmid#162587PurposeExpresses norovirus GI.1 VP1 protein with mutations N167C-L169C in Sf9 insect cellsDepositorInsertVP1 protein from human norovirus GI.1
UseTagsExpressionInsectMutationAsparagine 167 changed to Cysteine and Leucine 16ā¦PromoterpolyhedrinAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-OSF-TRIM5alpha(human)
Plasmid#79034PurposeBaculoviral entry vector to produce Bac-to-bac baculovirusesDepositorInsertOSF-TRIM5alpha(human) (TRIM5 Human)
UseTagsOneSTrEP-FLAGExpressionInsectMutationPromoterpolyhedrinAvailable sinceMarch 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-OSF-TRIM5alpha(chimpanzee)
Plasmid#79032PurposeBaculoviral entry vector to produce Bac-to-bac baculovirusesDepositorInsertOSF-TRIM5alpha(chimpanzee)
UseTagsOneSTrEP-FLAGExpressionInsectMutationPromoterpolyhedrinAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-OSF-TRIM5alpha(rhesus)
Plasmid#79033PurposeBaculoviral entry vector to produce Bac-to-bac baculovirusesDepositorInsertOSF-TRIM5alpha(rhesus)
UseTagsOneSTrEP-FLAGExpressionInsectMutationPromoterpolyhedrinAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-Kozak-rhTRIM5alpha-PFOS
Plasmid#79038PurposeBaculoviral entry vector to produce Bac-to-bac baculovirusesDepositorInsertrhesus TRIM5alha-PFOS
UseTagsOneSTrEP-FLAGExpressionInsectMutationPromoterpolyhedrinAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-NEDD4L_W2-W3-W4-HECT (349)
Plasmid#187721PurposeExpress GST-tagged NEDD4L W2-W3-W4-HECT (349) in insect cellsDepositorInsertNEDD4L residues 349-975 (NEDD4L Human)
UseTagsGST-HRV 3C siteExpressionInsectMutationdeletion of residues 1-348PromoterAvailable sinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-His6-E3(1)-CUbo-TwinStrep
Plasmid#212712PurposeExpression of His6-E3(1)-CUbo-TwinStrep in insect cellsDepositorInsertE3(1)
UseTags6xHis and CUbo-TwinStrepExpressionBacterialMutationPromoterAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-Clover4-LUMABS-S1 RBD
Plasmid#183491PurposeTo express the Clover4-LUMABS-S1 RBD sensor for SARS-CoV-2 antibody dection.DepositorInsertClover4-LUMABS-S1 RBD
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-OSF-TRIM5alpha(human)(R332P)
Plasmid#79037PurposeBaculoviral entry vector to produce Bac-to-bac baculovirusesDepositorInsertOSF-TRIM5alpha(human R332P) (TRIM5 Human)
UseTagsOneSTrEP-FLAGExpressionInsectMutationChanged Arg 332 to ProPromoterpolyhedrinAvailable sinceMarch 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
FLAG-mFzd4-opt(40-537)-9xHis_pFastBac1
Plasmid#216378PurposeBaculovirus transfer vector to express FLAG-tagged mouse Fzd4 (codon-optimized for insect cell expression)DepositorInsertFrizzled-4 (Fzd4 Mouse)
UseBaculovirusTagsHA signal sequence-FLAGExpressionInsectMutationPromoterPolyhedrinAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-OSF-TRIMCyp (owl monkey)
Plasmid#79035PurposeBaculoviral entry vector to produce Bac-to-bac baculovirusesDepositorInsertOSF-TRIM5Cyp (owl monkey)
UseTagsOneSTrEP-FLAGExpressionInsectMutationPromoterpolyhedrinAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-OSF-TRIM5alpha(African green monkey pygerythrus)
Plasmid#79031PurposeBaculoviral entry vector to produce Bac-to-bac baculovirusesDepositorInsertOSF-TRIM5alpha(African green monkey pygerythrus)
UseTagsOneSTrEP-FLAGExpressionInsectMutationPromoterpolyhedrinAvailable sinceMarch 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-OSF-TRIMCyp (owl monkey)(K283D, Q287D)
Plasmid#79036PurposeBaculoviral entry vector to produce Bac-to-bac baculovirusesDepositorInsertOSF-TRIM5Cyp (owl monkey) (K283D, Q287D)
UseTagsOneSTrEP-FLAGExpressionInsectMutationChanged Lys 283 to Asp and Gln 287 to AspPromoterpolyhedrinAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1 human full-length CENP-E-mNeon-6xHis
Plasmid#180484PurposepFastBac1 human CENP-E-mNeon-His, containing a 3c protease cleavage site between mNeon and His tag. Codon optimized for expression in Sf9 cellsDepositorInsertCENP-E (CENPE Human, Synthetic, human)
UseTagsmNeon-6xHisExpressionInsectMutationPromoterpolyhedrin promoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-OSF-TRIM5alpha(African green monkey pygerythrus)(1-293)
Plasmid#79030PurposeBaculoviral entry vector to produce Bac-to-bac baculovirusesDepositorInsertOSF-TRIM5alpha(African green monkey pygerythrus)(1-293)
UseTagsOneSTrEP-FLAGExpressionInsectMutationdeleted amino acid 294-515 from frican green monkā¦PromoterpolyhedrinAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pDest-795
Plasmid#161881PurposeStandard Gateway (attR1-attR2) vector for expression of maltose-binding protein (MBP) fusions using the Bac-to-Bac insect cell systemDepositorTypeEmpty backboneUseTagsMBPExpressionInsectMutationPromoterAvailable sinceJan. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDest-633
Plasmid#161877PurposeStandard Gateway (attR1-attR2) vector for expression of His6-GST fusions using the Bac-to-Bac insect cell systemDepositorTypeEmpty backboneUseTagsHis6-GSTExpressionInsectMutationPromoterAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only