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Showing: 1 - 20 of 231 results

  1. pFastBac1-melHis10TEV

    Plasmid
    #159428
    Purpose
    empty pFastBac1 secretion vector (polyhedrin promoter) adds N-term melittin signal sequence & TEV-protease-removable His10; avoids unwanted residues in final protein by using BseRI restriction sites
    Depositor
    Debra Hansen
    Type
    Empty backbone
    Use
    Tags
    melittin signal sequence (removed in vivo by sign…
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin
    Available since
    June 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    159428-plasmid-map-sequence-id-333931

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  2. pFastBac1-His10FLAG

    Plasmid
    #157736
    Purpose
    empty pFastBac1 vector (polyhedrin promoter) adds enterokinase-removable N-terminal His10-tag + 3xFLAG tag; avoids unwanted residues in the final protein sequence by using the BseRI restriction sites
    Depositor
    Debra Hansen
    Article
    Olson et al Protein Expr Purif. 2021 May 7:105890. d
    Type
    Empty backbone
    Use
    Tags
    His10 + 3xFLAG, enterokinase-cleavable
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin
    Available since
    June 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    157736-plasmid-map-sequence-id-333926

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  3. pFastBac1-pGC-A

    Plasmid
    #186626
    Purpose
    pFastBac1 (baculovirus polyhedrin promoter) encoding HA signal peptide, TEV-protease cleavable His10-tag, and full-length human pGC-A (NP_000897.3 amino acids 33-1061; expression-optimized DNA)
    Depositor
    Debra Hansen
    Article
    Zhang et al Sci Rep. 2022 Jul 12;12(1):11824. doi: 1
    Insert
    NPR1 (NPR1 Human)
    Use
    Tags
    hemagglutinin (HA) signal peptide + His10-tag + T…
    Expression
    Insect
    Mutation
    deleted amino acids 1-32
    Promoter
    polyhedring
    Available since
    July 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    186626-plasmid-map-sequence-id-366906

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  4. pFastBac1-His10TEV

    Plasmid
    #159427
    Purpose
    empty pFastBac1 vector (polyhedrin promoter) adds TEV-protease-removable N-terminal His10-tag; avoids unwanted residues in the final protein sequence by using the BseRI restriction sites
    Depositor
    Debra Hansen
    Article
    Olson et al Protein Expr Purif. 2021 May 7:105890. d
    Type
    Empty backbone
    Use
    Tags
    TEV-protease-cleavable His10-tag
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin
    Available since
    June 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    159427-plasmid-map-sequence-id-333995

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  8. pFastBac1-Nucleocapsid

    Plasmid
    #179696
    Purpose
    Expresses H6 tagged SARS CoV-2 nucleocapsid in Sf9 cells
    Depositor
    Mark Arbing
    Insert
    SARS-CoV-2 Nucleocapsid (N protein) (N Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    His6-TEV protease site
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin
    Available since
    Jan. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    179696-plasmid-map-sequence-id-352199

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  14. pFastBac1-C17orf53

    Plasmid
    #211445
    Purpose
    generation of Bacmid for insect cell expression of C17orf53
    Depositor
    Helmut Pospiech
    Insert
    C17orf53 (HROB Human)
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Jan. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    211445-plasmid-map-sequence-id-419422

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  15. pFastBac1_FLAG-BPTF

    Plasmid
    #102242
    Purpose
    Expression of human Flag-BPTF using baculovirus from pFastBac1
    Depositor
    Joe Landry
    Insert
    Flag-BPTF (BPTF Human)
    Use
    Tags
    Flag
    Expression
    Insect
    Mutation
    Promoter
    Polyhedron
    Available since
    Dec. 11, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    102242-plasmid-map-sequence-id-197767

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Showing: 1 - 20 of 231 results