1. pSpCas9(BB)-2A-Puro (PX459)

   Plasmid

   #48139

   Purpose

   NOTE: A new version of this plasmid is now available. See Addgene plasmid 62988.

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Insert

   hSpCas9-2A-Puro
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   Oct. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

These results may also be relevant.

1. pX459-dMET

   Plasmid

   #176031

   Purpose

   A knock-out vector for dog MET

   Depositor

   Michiyuki Matsuda

   Article

   Hino et al Dev Cell. 2022 Oct 10;57(19):2290-2304.e

   Insert

   A gRNA targeting the dog MET gene and the cDNA of Cas9 (MET )
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. PX459 mDnd1#1

   Plasmid

   #106345

   Purpose

   small guide RNA #1 against RRM-coding region of Dnd1 gene locus

   Depositor

   Thomas Tuschl

   Article

   Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

   Insert

   Dnd1 (Dnd1 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. PX459 mDnd1#2

   Plasmid

   #106346

   Purpose

   small guide RNA #2 against RRM-coding region of Dnd1 gene locus

   Depositor

   Thomas Tuschl

   Article

   Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

   Insert

   Dnd1 (Dnd1 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PX459 mDnd1#3

   Plasmid

   #106347

   Purpose

   small guide RNA #3 against RRM-coding region of Dnd1 gene locus

   Depositor

   Thomas Tuschl

   Article

   Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

   Insert

   Dnd1 (Dnd1 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pT7-V5-SBP-C1-HshnRNPA2-B1_Z

   Plasmid

   #148139

   Purpose

   Mammalian Expression of HshnRNPA2-B1

   Depositor

   Elisa Izaurralde

   Insert

   HshnRNPA2-B1 (HNRNPA2B1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   splicing variant of hnRNPA2 variant B1. Exon 6 an…

   Promoter

   Available since

   March 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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