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SIN40C.TRE.Luciferase.IRES.dTomato.PGK.sfGFP.P2A.Tet3G

Plasmid

#169317

Purpose

Doxycycline-inducible expression of Luciferase cDNA with dTomato as reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

Luciferase

Use

Lentiviral

Tags

IRES-dTomato

Expression

Mutation

Promoter

TRE

Available since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP_IRES_tdTomato

Plasmid

#184046

Purpose

Expresses EGFP and tdTomato in mammalian cells under control of a CMV promoter by using an IRES sequence

Depositor

Angela Pannier

Article

Kozisek et al J Biol Eng. 2023 Dec 7;17(1):76. doi: 10

Inserts

Enhanced Green Fluorescent Protein
tdTomato
Internal Ribosome Entry Site

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pMyrPalm_mEGFP_IRES_puro2b

Plasmid

#21038

Depositor

Daniel Gerlich

Article

Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

Insert

pMyrPalm

Use

Tags

mEGFP

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

May 13, 2009

Availability

Academic Institutions and Nonprofits only
SIN40C.TRE.MCS.IRES.dTomato.PGK.sfGFP.P2A.Tet3G

Plasmid

#169283

Purpose

Doxycycline-inducible overexpression of cDNA with dTomato as a reporter fluorescent protein

Depositor

Dirk Heckl

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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SIN40C.TRE.HA.RUNX1A.IRES.dTomato.PGK.sfGFP.P2A.Tet3G

Plasmid

#181977

Purpose

Human RUNX1A gene overexpression

Depositor

Jan-Henning Klusmann

Insert

RUNX1A (human) (RUNX1 Human)

Use

Lentiviral

Tags

HA (Human influenza hemagglutinin)

Expression

Mutation

Promoter

Available since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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pRRL.PPT.SFFV.HA.RUNX1A.IRES.eGFP

Plasmid

#181979

Purpose

Human RUNX1A gene overexpression

Depositor

Jan-Henning Klusmann

Insert

RUNX1A (human) (RUNX1 Human)

Use

Lentiviral

Tags

HA (Human influenza hemagglutinin)

Expression

Mutation

Promoter

Available since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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pIRES_EGFP_GA_KCNE1

Plasmid

#173160

Purpose

For mammalian expression of KCNE1 and creating variants

Depositor

Al George

Article

Kuenze et al Elife. 2020 Oct 23;9. pii: 57680. doi: 1

Insert

KCNE1 (KCNE1 Human)

Use

Tags

IRES2-EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 16, 2021

Availability

Academic Institutions and Nonprofits only

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SIN40C.TRE.ARID3a.IRES.dTomato.PGK.sfGFP.P2A.Tet3G

Plasmid

#169314

Purpose

Doxycycline-inducible expression of human ARID3A cDNA with dTomato as reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

ARID3A (ARID3A Human)

Use

Lentiviral

Tags

IRES-dTomato

Expression

Mutation

Promoter

TRE

Available since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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SIN40C.TRE.mArid3a3xFLAG.IRES.dTomato.PGK.sfGFP.P2A.Tet3G

Plasmid

#174666

Purpose

Doxycycline-inducible expression of murine Arid3a-3xFLAG cDNA with dTomato as reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

Arid3a-3xFLAG (Arid3a Mouse)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

TRE

Available since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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SIN40C.SFFV.ARID3a.IRES.GFP

Plasmid

#169300

Purpose

Constitutive overexpression of human ARID3A cDNA with GFP as reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

ARID3A (ARID3A Human)

Use

Lentiviral

Tags

IRES-EGFP

Expression

Mutation

Promoter

SFFV

Available since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

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SFG.CNa12_opt.IRES.eGFP

Plasmid

#22490

Depositor

Martin Pule

Article

Brewin et al Blood. 2009 Sep 21. ():.

Insert

Codon optimised Calcineurin A mutant 12 (PPP3CA Human)

Use

Retroviral

Tags

Expression

Mutation

T351E; L354A

Promoter

Available since

Dec. 16, 2009

Availability

Academic Institutions and Nonprofits only

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SIN40C.TRE.HA.RUNX1C.IRES.dTomato.PGK.sfGFP.P2A.Tet3G

Plasmid

#181978

Purpose

Human RUNX1C gene overexpression

Depositor

Jan-Henning Klusmann

Insert

RUNX1C (human) (RUNX1 Human)

Use

Lentiviral

Tags

HA (Human influenza hemagglutinin)

Expression

Mutation

Promoter

Available since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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pIRES.Puro.EGFP

Plasmid

#24176

Depositor

Robert Sobol

Article

Jelezcova et al Mutat Res. 2010 Jan 22. ():.

Insert

EGFP

Use

Tags

Expression

Mammalian

Mutation

x

Promoter

Available since

April 9, 2010

Availability

Academic Institutions and Nonprofits only
pmPAGFP_IRES_puro2

Plasmid

#21046

Depositor

Daniel Gerlich

Article

Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

Insert

pmPAGFP

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

May 15, 2009

Availability

Academic Institutions and Nonprofits only
SIN40C.SFFV.mArid3a.IRES.GFP

Plasmid

#169309

Purpose

Constitutive overexpression of murine Arid3a cDNA with GFP as reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

Arid3a (Arid3a Mouse)

Use

Lentiviral

Tags

IRES-EGFP

Expression

Mutation

Promoter

SFFV

Available since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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SFG.CNa22_opt.IRES.eGFP

Plasmid

#22491

Depositor

Martin Pule

Article

Brewin et al Blood. 2009 Sep 21. ():.

Insert

Codon optimised Calcineurin A mutant 22 (PPP3CA Human)

Use

Retroviral

Tags

Expression

Mutation

V314R; Y341F

Promoter

Available since

Dec. 16, 2009

Availability

Academic Institutions and Nonprofits only

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pFoxd3IRESGFPtv

Plasmid

#37266

Depositor

Patricia Labosky

Article

Hanna et al Genes Dev. 2002 Oct 15;16(20):2650-61.

Insert

Foxd3 (Foxd3 Mouse)

Use

Mouse Targeting

Tags

IRES-GFP

Expression

Mutation

Promoter

Available since

Aug. 1, 2012

Availability

Academic Institutions and Nonprofits only
pUC_cohNaV1.1_2009aa_IRES2_GFP

Plasmid

#227642

Purpose

Expresses Nav 1.1 and IRES-GFP

Depositor

Dravet Syndrome Foundation Spain

Insert

NaV1.1 (SCN1A Human)

Use

Tags

IRES-GFP

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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SIN40C.TRE.mArid3a.IRES.dTomato.PGK.sfGFP.P2A.Tet3G

Plasmid

#169318

Purpose

Doxycycline-inducible expression of murine Arid3a cDNA with dTomato as reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

Arid3a (Arid3a Mouse)

Use

Lentiviral

Tags

IRES-dTomato

Expression

Mutation

Promoter

TRE

Available since

Sept. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-protein1-IRES-protein2-IRESatt-EGFP-2A-PuroR-SMAR-g5

Plasmid

#208377

Purpose

Episomal plasmid for expression of 2 proteins with puromycin selection and EGFP monitoring

Depositor

Daniel Hawiger

Article

Fallahee et al Antibodies (Basel). 2024 Mar 11;13(1):18

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

human CMV

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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