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CoV2-Spike-D614G-L452R

Plasmid

#177971

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, L452R)

Depositor

Melanie Ott

Article

Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

Insert

SARS-CoV-2 Spike protein (D614G, L452R) (S )

Use

Tags

Expression

Mammalian

Mutation

D614G, L452R

Promoter

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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CoV2-Spike-D614G-S477N

Plasmid

#177972

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, S477N)

Depositor

Melanie Ott

Article

Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

Insert

SARS-CoV-2 Spike protein (D614G, S477N) (S )

Use

Tags

Expression

Mammalian

Mutation

D614G, S477N

Promoter

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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CoV2-Spike-D614G-N501Y

Plasmid

#177975

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, N501Y)

Depositor

Melanie Ott

Article

Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

Insert

SARS-CoV-2 Spike protein (D614G, N501Y) (S )

Use

Tags

Expression

Mammalian

Mutation

D614G, N501Y

Promoter

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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CoV2-Spike-D614G-S13I

Plasmid

#177965

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, S13I)

Depositor

Melanie Ott

Article

Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

Insert

SARS-CoV-2 Spike protein (D614G, S13I) (S )

Use

Tags

Expression

Mammalian

Mutation

D614G, S13I

Promoter

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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CoV2-Spike-D614G-T20N

Plasmid

#177966

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, T20N)

Depositor

Melanie Ott

Article

Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

Insert

SARS-CoV-2 Spike protein (D614G, T20N) (S )

Use

Tags

Expression

Mammalian

Mutation

D614G, T20N

Promoter

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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CoV2-Spike-D614G-P26S

Plasmid

#177967

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, P26S)

Depositor

Melanie Ott

Article

Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

Insert

SARS-CoV-2 Spike protein (D614G, P26S) (S )

Use

Tags

Expression

Mammalian

Mutation

D614G, P26S

Promoter

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
CoV2-Spike-D614G-W152C

Plasmid

#177968

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, W152C)

Depositor

Melanie Ott

Article

Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

Insert

SARS-CoV-2 Spike protein (D614G, W152C) (S )

Use

Tags

Expression

Mammalian

Mutation

D614G, W152C

Promoter

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
CoV2-Spike-D614G-P681H

Plasmid

#177973

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, P681H)

Depositor

Melanie Ott

Article

Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

Insert

SARS-CoV-2 Spike protein (D614G, P681H) (S )

Use

Tags

Expression

Mammalian

Mutation

D614G, P681H

Promoter

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
CoV2-Spike-D614G-D1118H

Plasmid

#177974

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (D614G, D1118H)

Depositor

Melanie Ott

Article

Deng et al Cell. 2021 Jun 24;184(13):3426-3437.e8.

Insert

SARS-CoV-2 Spike protein (D614G, D1118H) (S )

Use

Tags

Expression

Mammalian

Mutation

D614G, D1118H

Promoter

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pOTTC155

Plasmid

#50949

Purpose

An LV packaging vector expressing eGFP

Depositor

Brandon Harvey

Insert

eGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pUC19-T7-Bottom-T

Plasmid

#87310

Purpose

Expresses the "Bottom" transcript of the Split-Broccoli system under the control of the T7 promoter.

Depositor

Donald Burke

Article

Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

Insert

Split-Broccoli system "Bottom" transcript

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 27, 2018

Availability

Academic Institutions and Nonprofits only

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pOTTC590 - pCMV ER-iRFP

Plasmid

#59136

Purpose

A plasmid that express endoplasmic reticulum-targeted iRFP under the CMV promoter.

Depositor

Brandon Harvey

Article

Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

Insert

ER-localized Infrared Fluorescent Protein

Use

Tags

ER retention signal and signal peptide

Expression

Mammalian

Mutation

Promoter

CMV-IE

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pUC19-P70a-Bottom-T

Plasmid

#87313

Purpose

Expresses the "Bottom" transcript of the Split-Broccoli system under the control of the E. coli sigma 70 promoter.

Depositor

Donald Burke

Article

Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

Insert

Split-Broccoli system "Bottom" transcript

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

P70a

Available since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC19-Trigger-Bottom

Plasmid

#87317

Purpose

Expresses the "Bottom" transcript of the Split-Broccoli system downstream of an RNA Toehold switch Trigger sequence.

Depositor

Donald Burke

Article

Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

Insert

Split-Broccoli "Bottom" transcript downstream of RNA Trigger (TS2_AT01)

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

P70a

Available since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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pAM-PAT-ProTTG1 GW

Plasmid

#79753

Purpose

binary DEST vector for plant expression

Depositor

Martin Hulskamp, Nico Dissmeyer

Article

Bouyer et al PLoS Biol. 2008 Jun 10;6(6):e141. doi: 1

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

ProTTG1

Available since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pOTTC1484 - pAAV SYN1 HA-hM4D(Gi)

Plasmid

#121538

Purpose

An adeno-associated viral vector expressing HA-tagged inhibitory DREADD (hM4D(Gi)) from a synapsin promoter

Depositor

Christopher Richie

Article

Galvan et al Eur J Neurosci. 2019 May 7. doi: 10.1111

Has Service

AAV2 and AAV5

Insert

HA-tagged hM4D(Gi) (CHRM4 Human)

Use

AAV

Tags

HA

Expression

Mammalian

Mutation

Promoter

SYN1

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pOTTC407 - pAAV EF1a eGFP

Plasmid

#60058

Purpose

An AAV packaging vector that expresses enhanced green fluorescent protein under control of the EF1a promoter.

Depositor

Brandon Harvey

Article

Savolainen et al Neurobiol Dis. 2014 Aug;68:1-15. doi: 10

Insert

enhanced Green Fluorescent Protein

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Oct. 9, 2014

Availability

Academic Institutions and Nonprofits only

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pOTTC1328 - pAAV EF1a HA-hM3D(Gq)

Plasmid

#89149

Purpose

An AAV packaging plasmid that expresses HA-tagged hM3D(Gq) excitatory DREADD from the EF1a promoter.

Depositor

Brandon Harvey

Article

Gomez et al Science. 2017 Aug 4;357(6350):503-507. d

Insert

hM3D(Gq)

Use

AAV

Tags

HA

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pOTTC476 - pAAV c-fos eYFP

Plasmid

#47907

Purpose

An AAV vector that expresses eYFP under the c-fos promoter.

Depositor

Brandon Harvey

Article

Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

Insert

enhanced Yellow Fluorescent Protein

Use

AAV

Tags

Expression

Mutation

Promoter

c-fos

Available since

Nov. 7, 2013

Availability

Academic Institutions and Nonprofits only

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pOTTC1329 - pAAV EF1a HA-hM4D(Gi)

Plasmid

#89150

Purpose

An AAV packaging plasmid that expresses HA-tagged hM4D(Gi) inhibitory DREADD from the EF1a promoter.

Depositor

Brandon Harvey

Article

Gomez et al Science. 2017 Aug 4;357(6350):503-507. d

Insert

hM4D(Gi)

Use

AAV

Tags

HA

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge