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Plasmid#231376PurposeExpress mutant human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cellsDepositorInsertHuman Argonaute2 (AGO2 Synthetic, Human)
UseTags3xFLAG-SUMO_Eu1ExpressionMammalianMutationK98APromoterCMVAvailable sinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(R710A)
Plasmid#231381PurposeExpress mutant human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cellsDepositorInsertHuman Argonaute2 (AGO2 Synthetic, Human)
UseTags3xFLAG-SUMO_Eu1ExpressionMammalianMutationR710APromoterCMVAvailable sinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(H712A)
Plasmid#231382PurposeExpress mutant human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cellsDepositorInsertHuman Argonaute2 (AGO2 Synthetic, Human)
UseTags3xFLAG-SUMO_Eu1ExpressionMammalianMutationH712APromoterCMVAvailable sinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(R635A)
Plasmid#231384PurposeExpress mutant human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cellsDepositorInsertHuman Argonaute2 (AGO2 Synthetic, Human)
UseTags3xFLAG-SUMO_Eu1ExpressionMammalianMutationR635APromoterCMVAvailable sinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(EI 5xE)
Plasmid#231385PurposeExpress mutant human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cellsDepositorInsertHuman Argonaute2 (AGO2 Synthetic, Human)
UseTags3xFLAG-SUMO_Eu1ExpressionMammalianMutationS824E, S828E, T830E, S831E, S834EPromoterCMVAvailable sinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(D669A)
Plasmid#231371PurposeExpress catalytically dead mutant human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cellsDepositorInsertHuman Argonaute2 (AGO2 Synthetic, Human)
UseTags3xFLAG-SUMO_Eu1ExpressionMammalianMutationD669APromoterCMVAvailable sinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-hsAgo2
Plasmid#221718PurposeExpression of human Ago2 in zebrafish embryos.DepositorInserthsAgo2 (AGO2 Human)
UseFish expressionTagsExpressionMutationPromotersimian CMV IE94Available sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSAG1::CAS9-U6::sgUPRT
Plasmid#54467PurposeEncodes CAS9 nuclease (GFP fusion) under control of the Toxoplasma gondii SAG1 promotor. Vector also provides U6 controlled expression of a single guide RNA for disruption of genes by CRISPR.DepositorInsertSAG1 5' UTR
UseCRISPR; Toxoplasma expressionTagsExpressionMutationPromoterAvailable sinceJune 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsAGO1_F
Plasmid#146314PurposeMammalian Expression of HsAGO1DepositorInsertHsAGO1 (AGO1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pU6-SAG1-DHFR
Plasmid#80322PurposeEncodes a CRISPR sgRNA that allows for mutation of the surface antigen SAG1 in Toxoplasma gondii and confers resistance to pyrimethamineDepositorInsertSAG1 sgRNA
UseCRISPRTagsExpressionMutationPromoterU6Available sinceSept. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMPMA3dPlac-PssaG-GFPLVA
Plasmid#23343DepositorInsertGFP(LVA)
UseTagsGFP(LVA)ExpressionBacterialMutationPromoterAvailable sinceApril 27, 2010AvailabilityAcademic Institutions and Nonprofits only -
pSAG1::CAS9-U6::sg290860-6
Plasmid#59855PurposeEncodes CAS9 nuclease (GFP fusion) under control of the Toxoplasma gondii SAG1 promotor. Vector also provides U6 controlled expression of a single guide RNA for CRISPR disruption of TGME49_290860.DepositorInsertCRISPR sg290860-6
UseCRISPR; ; toxoplasma gondiiTagsExpressionMutationPromoterAvailable sinceOct. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsAGO2_F
Plasmid#146315PurposeMammalian Expression of HsAGO2DepositorInsertHsAGO2 (AGO2 Human)
UseTagsExpressionMammalianMutationone silent mutation C840T and sequence differs in…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsAGO3_F
Plasmid#146316PurposeMammalian Expression of HsAGO3DepositorInsertHsAGO3 (AGO3 Human)
UseTagsExpressionMammalianMutationT25APromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(H56A, K98A)
Plasmid#231377PurposeExpress mutant human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cellsDepositorInsertHuman Argonaute2 (AGO2 Synthetic, Human)
UseTags3xFLAG-SUMO_Eu1ExpressionMammalianMutationH56A, K98APromoterCMVAvailable sinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(R97A, K98A)
Plasmid#231378PurposeExpress mutant human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cellsDepositorInsertHuman Argonaute2 (AGO2 Synthetic, Human)
UseTags3xFLAG-SUMO_Eu1ExpressionMammalianMutationR97A, K98APromoterCMVAvailable sinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(R97E, K98E)
Plasmid#231380PurposeExpress mutant human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cellsDepositorInsertHuman Argonaute2 (AGO2 Synthetic, Human)
UseTags3xFLAG-SUMO_Eu1ExpressionMammalianMutationR97E, K98EPromoterCMVAvailable sinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3_3xFLAG_SUMO_Eu1_HsAGO2(R710A, H712A)
Plasmid#231383PurposeExpress mutant human AGO2 with N-terminal 3xFLAG-SUMO_Eu1 tag in mammalian cellsDepositorInsertHuman Argonaute2 (AGO2 Synthetic, Human)
UseTags3xFLAG-SUMO_Eu1ExpressionMammalianMutationR710A, H712APromoterCMVAvailable sinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-FLAG3-hsAgo2EF-DY
Plasmid#221723PurposeExpression of human Ago2 with E637D and F666Y mutations and N-terminal 3xFLAG tag in zebrafish embryos.DepositorInserthsAgo2EF-DY (AGO2 Human)
UseFish expressionTags3xFLAGExpressionMutationE637D and F666Y in active sitePromotersimian CMV IE94Available sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCS2+-FLAG3-hsAgo2F-Y
Plasmid#221722PurposeExpression of human Ago2 with F666Y mutation and N-terminal 3xFLAG tag in zebrafish embryos.DepositorInserthsAgo2F-Y (AGO2 Human)
UseFish expressionTags3xFLAGExpressionMutationF666Y in active sitePromotersimian CMV IE94Available sinceOct. 15, 2024AvailabilityAcademic Institutions and Nonprofits only