We narrowed to 236 results for: Weissman Jonathan

1. pMJ117

   Plasmid

   #85997

   Purpose

   One-guide Perturb-seq vector backbone; modified human U6 promoter; constant region 3

   Depositor

   Jonathan Weissman

   Article

   Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

   Inserts

   EGFP-NT2_cr3
   puro-T2A-BFP
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   modified human U6

   Available Since

   Feb. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMJ179

   Plasmid

   #85996

   Purpose

   One-guide Perturb-seq vector backbone; modified mouse U6 promoter; constant region 2

   Depositor

   Jonathan Weissman

   Article

   Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

   Inserts

   EGFP-NT2_cr2
   puro-T2A-BFP
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   modified mouse U6

   Available Since

   Feb. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBA900

   Plasmid

   #122237

   Purpose

   Lentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs2 incorporated at the 3' end of the sgRNA constant region.

   Depositor

   Jonathan Weissman

   Article

   Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.

   Insert

   sgRNA with cs2 at the 3' end of the constant region
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBA950

   Plasmid

   #122239

   Purpose

   CROP-seq vector optimized for sgRNA expression and CRISPRi activity. This vector expresses a eGFP-NT2 sgRNA with modified U6 promotor and sgRNA constant region.

   Depositor

   Jonathan Weissman

   Article

   Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.

   Insert

   BFP selectable marker, plus a modified mU6 promoter and sgRNA constant region for optimized CRISPRi activity
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   April 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTMB301_ZFcharm_Kv1_Prnp_SPM

   Plasmid

   #220840

   Purpose

   AAV genome expressing ZFcharm Kv1 targeting Prnp; single perfect match self-silencing binding site

   Depositor

   Jonathan Weissman

   Article

   Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

   Insert

   ZFcharm Kv1
   

   Use

   AAV

   Tags

   HAtag

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   EFS

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBA407

   Plasmid

   #85970

   Purpose

   UPRE-mCh-Ubc-Neo

   Depositor

   Jonathan Weissman

   Article

   Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

   Inserts

   mCherry
   Neo
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Feb. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAG26

   Plasmid

   #226743

   Purpose

   Expresses a reporter for a mitochondrial intermembrane space protein

   Depositor

   Jonathan Weissman

   Article

   Guna et al Science. 2022 Oct 21;378(6617):317-322.

   Insert

   MICU1 (targeting sequence) (MICU1 Human)
   

   Use

   Lentiviral

   Tags

   GFP1-10

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAG496

   Plasmid

   #226750

   Purpose

   Expresses exogenous MTCH2

   Depositor

   Jonathan Weissman

   Article

   Guna et al Science. 2022 Oct 21;378(6617):317-322.

   Insert

   MTCH2 (MTCH2 Human)
   

   Use

   Lentiviral

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBA409

   Plasmid

   #85971

   Purpose

   EF1a-sfGFP-Ubc-Neo

   Depositor

   Jonathan Weissman

   Article

   Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

   Inserts

   sfGFP
   Neo
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Feb. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJW1512

    Plasmid

    #62366

    Purpose

    N-terminal 3X FLAG fused to wt BirA - mCherry - sec61ß

    Depositor

    Jonathan Weissman

    Article

    Jan et al Science. 2014 Nov 7;346(6210):1257521. d

    Insert

    3xFLAG-BirA-mCherry-Sec61beta tail anchor (SEC61B Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    pTRE2

    Available Since

    April 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTMB304_ZFcharm_Kv1_Prnp_SCR

    Plasmid

    #220842

    Purpose

    AAV genome expressing ZFcharm Kv1 targeting Prnp; scrambled self-silencing binding site

    Depositor

    Jonathan Weissman

    Article

    Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

    Insert

    ZFcharm Kv1
    

    Use

    AAV

    Tags

    HAtag

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    EFS

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGM227

    Plasmid

    #220182

    Purpose

    Expresses exogenous wild-type TOMM70A

    Depositor

    Jonathan Weissman

    Article

    Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

    Insert

    TOMM70A (TOMM70 Human)
    

    Use

    Lentiviral

    Available Since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. PCT62

    Plasmid

    #170391

    Purpose

    expresses three sgRNA cassettes

    Depositor

    Jonathan Weissman

    Article

    Quinn et al Science. 2021 Feb 26;371(6532). pii: sci

    Insert

    PCT62-targetsite
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGM234

    Plasmid

    #220177

    Purpose

    Expresses exogenous wild-type NAC complex members (NACA and BTF3)

    Depositor

    Jonathan Weissman

    Article

    Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

    Insert

    NACA and BTF3 (NACA, BTF3 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG (NACA); 6xHis (BTF3)

    Available Since

    June 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAG491

    Plasmid

    #226747

    Purpose

    CRISPRi guide RNA against MTCH2 (dual guide)

    Depositor

    Jonathan Weissman

    Article

    Guna et al Science. 2022 Oct 21;378(6617):317-322.

    Insert

    MTCH2 (MTCH2 Human)
    

    Use

    Lentiviral

    Available Since

    Nov. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAG397

    Plasmid

    #226733

    Purpose

    Expresses a reporter for an alpha-helical OMM protein

    Depositor

    Jonathan Weissman

    Article

    Guna et al Science. 2022 Oct 21;378(6617):317-322.

    Insert

    OMP25 (SYNJ2BP Human)
    

    Use

    Lentiviral

    Tags

    GFP11

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAG43

    Plasmid

    #226736

    Purpose

    Expresses a reporter for an alpha-helical OMM protein

    Depositor

    Jonathan Weissman

    Article

    Guna et al Science. 2022 Oct 21;378(6617):317-322.

    Insert

    MICU1 (targeting sequence) (MICU1 Human)
    

    Use

    Lentiviral

    Tags

    GFP11

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGM238

    Plasmid

    #220178

    Purpose

    Expresses exogenous ribosomal-binding domain mutant NAC complex members (K78E NACA and K43E BTF3)

    Depositor

    Jonathan Weissman

    Article

    Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

    Insert

    NACA and BTF3 (NACA, BTF3 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG (NACA); 6xHis (BTF3)

    Mutation

    K78E (NACA) + K43B (BTF3)

    Available Since

    June 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDYT001

    Plasmid

    #186549

    Purpose

    Lineage tracing vector (with barcoded Target Sites and triple sgRNAs)

    Depositor

    Jonathan Weissman

    Article

    Yang et al Cell. 2022 Apr 28. pii: S0092-8674(22)00

    Insert

    14-bp random integration barcode and three target sites and 3x sgRNAs
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    Aug. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJW1661

    Plasmid

    #112035

    Purpose

    OsTIR1 expression cassette marked with URA

    Depositor

    Jonathan Weissman

    Article

    Costa et al Science. 2018 Jan 18. pii: science.aar36

    Insert

    TIR1
    

    Expression

    Yeast

    Available Since

    Sept. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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