We narrowed to 47 results for: fucci

1. Fucci(CA) hCdt1-iRFP hGeminin-iRFP

   Plasmid

   #190182

   Purpose

   Fluorescent reporter vector for visualization of G0 and other cell cycle phases.

   Depositor

   Toshiro Sato

   Article

   Ohta et al Nature. 2022 Jul 7. pii: 10.1038/s41586-

   Inserts

   iRFP
   hCdt1(1/100)Cy(-) (Cdt1 Synthetic)
   iRFP
   hGeminin(1/110)
   

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV[Exp]-Hygro-CMV-FUCCIplex

   Plasmid

   #240221

   Purpose

   Multiplexable FUCCI sensor (FUCCIplex). The CMV promoter drives the expression of the fluorescent sensor.

   Depositor

   Francesco Pasqualini

   Article

   Di Sante et al bioRxiv 2024.12.19.629259

   Insert

   FUCCIplex
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pME-zFUCCI-P2A-H2A-tdiRFP (JDW 1380)

   Plasmid

   #242559

   Purpose

   Gateway middle entry clone containing a zebrafish FUCCI reporter followed by H2A tdiRFP reporter; For visualizing cell cycle state.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   mCerulean-zGeminin(1-100) P2A mCherry-zCdt1(1-190)
   

   Use

   Gateway subcloning

   Available Since

   Sept. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-PGK-Neo-PIP-NLS-mVenus

   Plasmid

   #118619

   Purpose

   Lentiviral expression of the cell cycle marker PIP-NLS-mVenus

   Depositor

   Jean Cook

   Article

   Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

   Insert

   PIP-NLS-mVenus
   

   Use

   Lentiviral

   Tags

   mVenus

   Expression

   Mammalian

   Promoter

   hPGK

   Available Since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-PGK-Puro-TK-NLS-mTurq2-PCNA

   Plasmid

   #118617

   Purpose

   Lentiviral expression of mTurq2 tagged PCNA

   Depositor

   Jean Cook

   Article

   Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

   Insert

   TK-NLS-mTurq2-PCNA (PCNA Human)
   

   Use

   Lentiviral

   Tags

   mTurq2

   Expression

   Mammalian

   Promoter

   hPGK

   Available Since

   May 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDNA5/FRT/Halo-Tag7-Geminin(1/110)_P2A_HaloTag9-Cdt(1-100)Cy+

   Plasmid

   #169337

   Purpose

   Expression of the LT-Fucci(SCA) in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   Fucci(SCA) (GMNN, CDT1 Human)
   

   Tags

   HaloTag7-Geminin(1/110) and HaloTag9-Cdt(1-100)Cy…

   Expression

   Mammalian

   Mutation

   HaloTag9 = HaloTag7-Q165H-P174R

   Available Since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA5/FRT/HaloTag7-Geminin(1/110)_P2A_HaloTag9-Cdt(1-110)Cy-

   Plasmid

   #169338

   Purpose

   Expression of the LT-Fucci(CA) in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   Fucci(CA) (GMNN, CDT1 Human)
   

   Tags

   HaloTag7-Geminin(1/110) and HaloTag9-Cdt(1-110)Cy…

   Expression

   Mammalian

   Mutation

   HaloTag9 = HaloTag7-Q165H-P174R

   Available Since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA5/FRT/HaloTag7-Geminin(1/110)_P2A_HaloTag9-Cdt(30-120)

   Plasmid

   #169336

   Purpose

   Expression of the LT-Fucci(SA) in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   Fucci(SA) (GMNN, CDT1 Human)
   

   Tags

   HaloTag7-Geminin(1/110) and HaloTag9-Cdt(30-120)

   Expression

   Mammalian

   Mutation

   HaloTag9 = HaloTag7-Q165H-P174R

   Available Since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pITGA5_1745G>U

   Plasmid

   #173812

   Purpose

   mir218 binding site2 point mutation

   Depositor

   Andrew M. Siedlecki

   Article

   Liu et al J Am Soc Nephrol. 2022 Mar;33(3):565-582

   Insert

   NT1745 ITGA5 point mutation (ITGA5 Human)
   

   Expression

   Mammalian

   Promoter

   AmpR

   Available Since

   Sept. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pITGA5_473G>A

    Plasmid

    #173811

    Purpose

    mir218 binding site1 point mutation

    Depositor

    Andrew M. Siedlecki

    Article

    Liu et al J Am Soc Nephrol. 2022 Mar;33(3):565-582

    Insert

    NT473 ITGA5 point mutation (ITGA5 Human)
    

    Expression

    Mammalian

    Promoter

    AmpR

    Available Since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMIR218-2_nt5G>U

    Plasmid

    #174009

    Purpose

    miR218 nt5 point mutation in SLIT3

    Depositor

    Andrew M. Siedlecki

    Article

    Liu et al J Am Soc Nephrol. 2022 Mar;33(3):565-582

    Insert

    SLIT3 (SLIT3, MIR218-2 Human)
    

    Expression

    Mammalian

    Mutation

    NT5 G>U point mutation in SLIT3

    Available Since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pITGA5_Q853L

    Plasmid

    #174008

    Purpose

    Fibronectin receptor binding site point mutation

    Depositor

    Andrew M. Siedlecki

    Article

    Liu et al J Am Soc Nephrol. 2022 Mar;33(3):565-582

    Insert

    FibronR ITGA5 point mutation (ITGA5 Human)
    

    Expression

    Mammalian

    Promoter

    AmpR

    Available Since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAVS1_Puro_hPGK1_hGEM(1-110)_mNG_hSLBP(18-108)

    Plasmid

    #176932

    Purpose

    Donor for targeted integration of S-phase specific probe (FUCCI-S) to the AAVS1 safe harbor locus

    Depositor

    Yannick Doyon

    Insert

    hGEM(1-110)_mNG_hSLBP(18-108)
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    PGK1

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAVS1_Puro_hPGK1_hGEM(1-110)_mNG_hSLBP(18-108)_FS

    Plasmid

    #191527

    Purpose

    Donor for targeted integration of 3xFLAG-2xSTREP tagged S-phase specific probe (FUCCI-S) to the AAVS1 safe harbor locus

    Depositor

    Yannick Doyon

    Insert

    hGEM(1-110)_mNG_hSLBP(18-108)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    mNeonGreen (mNG) + 3xFLAG_TwinStrep

    Expression

    Mammalian

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCCL-CellCycle

    Plasmid

    #132429

    Purpose

    Expressing Fucci cell cycle indicator in mammalian cells

    Depositor

    John M. Greally

    Article

    Sato et al J Cell Biol. 2019 Jun 3;218(6):1810-1823

    Insert

    hCdt, hGeminin
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. Ac5-STABLE2-RFP-NLS-CycB(1-266)_GFP-E2F1(1-230)_neo

    Plasmid

    #73164

    Purpose

    multi-cistronic Vector for expression for Fly_FUCCI probes in insect cells

    Depositor

    Bruce Edgar

    Article

    Zielke et al Cell Rep. 2014 Apr 24;7(2):588-98. doi:

    Insert

    mRFP-CycB(1-266)-T2A-GFP-E2F1(1-230)-T2A-neoR (E2f1, CycB Fly)
    

    Tags

    mRFP1, GFP

    Expression

    Insect

    Mutation

    Cyclin B Fragment aa 1-266 & E2F1 Fragment aa…

    Available Since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAVS1-Puro CAG eGFP NLS

    Plasmid

    #170830

    Purpose

    Donor plasmid for knocking eGFP NLS into AAVS1 safe harbor locus

    Depositor

    Xiaoping Bao

    Article

    Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

    Insert

    eGFP
    

    Use

    CRISPR, Synthetic Biology, and TALEN

    Tags

    NLS

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. MultiMate-Cell-cycle

    Plasmid

    #206280

    Purpose

    Expression of a FUCCI cell cycle sensor with a H2B iRFP713 chromatin reporter. Can be used to generate recombinant baculovirus particles.

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    H2B iRFP713, mAG-hGeminin, mKO2-hCdt1
    

    Use

    Recombinant baculovirus production

    Expression

    Mammalian

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAVS1-Puro CAG IL13-CAR

    Plasmid

    #157742

    Purpose

    knock IL13-CAR into AAVS1 safe harbor locus for constitutive expression

    Depositor

    Xiaoping Bao

    Article

    Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

    Insert

    Chimeric antigen receptor targeting IL13
    

    Use

    CRISPR, Synthetic Biology, and TALEN

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. AAVS1-Puro CAG CLTX-CAR

    Plasmid

    #157743

    Purpose

    knock CLTX-CAR into AAVS1 safe harbor locus for constitutive expression

    Depositor

    Xiaoping Bao

    Article

    Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

    Insert

    chlorotoxin containing chimeric antigen receptor targeting glioblastoma
    

    Use

    CRISPR, Synthetic Biology, and TALEN

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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