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pRF TpC RE8

Plasmid

#171047

Purpose

Firefly-Renilla luciferase assay for hTERT promoter activity of RE8

Depositor

Agnel Sfeir

Article

Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

Insert

RE8

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pRF TpC RE2

Plasmid

#171041

Purpose

Firefly-Renilla luciferase assay for hTERT promoter activity of RE2

Depositor

Agnel Sfeir

Article

Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

Insert

RE2

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pRF TpC RE4

Plasmid

#171043

Purpose

Firefly-Renilla luciferase assay for hTERT promoter activity of RE4

Depositor

Agnel Sfeir

Article

Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

Insert

RE4

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pRF TpC RE1

Plasmid

#171040

Purpose

Firefly-Renilla luciferase assay for hTERT promoter activity of RE1

Depositor

Agnel Sfeir

Article

Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

Insert

RE1

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pRF TpC RE3

Plasmid

#171042

Purpose

Firefly-Renilla luciferase assay for hTERT promoter activity of RE3

Depositor

Agnel Sfeir

Article

Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

Insert

RE3

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pRF TpC RE5

Plasmid

#171044

Purpose

Firefly-Renilla luciferase assay for hTERT promoter activity of RE5

Depositor

Agnel Sfeir

Article

Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

Insert

RE5

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pRF TpC RE6

Plasmid

#171045

Purpose

Firefly-Renilla luciferase assay for hTERT promoter activity of RE6

Depositor

Agnel Sfeir

Article

Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

Insert

RE6

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pRF TpC RE7

Plasmid

#171046

Purpose

Firefly-Renilla luciferase assay for hTERT promoter activity of RE7

Depositor

Agnel Sfeir

Article

Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

Insert

RE7

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti pTERT sgRNA1(MS2) mCherry

Plasmid

#171054

Purpose

Targeting of Vp64-dCas9 activation system to hTERT promoter

Depositor

Agnel Sfeir

Article

Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

Insert

pTERT sgRNA1(MS2) mCherry

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti pTERT sgRNA2(MS2) mCherry

Plasmid

#171055

Purpose

Targeting of Vp64-dCas9 activation system to hTERT promoter

Depositor

Agnel Sfeir

Article

Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

Insert

pTERT sgRNA2(MS2) mCherry

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti sgRNA(MS2) mCherry

Plasmid

#171053

Purpose

Expression of Vp64-dCas9 activation system

Depositor

Agnel Sfeir

Article

Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

Insert

sgRNA(MS2) mCherry

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti Vp64-dCas9 T2A mTagBFP2

Plasmid

#171052

Purpose

Expression of Vp64-dCas9 activation system

Depositor

Agnel Sfeir

Article

Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

Insert

Vp64-dCas9 T2A mTagBFP2

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti MS2-p65-HSF1 sfGFP

Plasmid

#171051

Purpose

Expression of Vp64-dCas9 activation system

Depositor

Agnel Sfeir

Article

Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

Insert

MS2-p65-HSF1_sfGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTopo pLox hPGK-Puro pA pLox

Plasmid

#171048

Purpose

Empty donor template for CRISPR/Cas9 targeting of TERT RE's

Depositor

Agnel Sfeir

Article

Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTopo RE2 Donor pLox hPGK-Puro pA pLox

Plasmid

#171049

Purpose

Donor template for CRISPR/Cas9 targeting of TERT RE2

Depositor

Agnel Sfeir

Article

Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

Insert

Puromycin

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTopo RE3 Donor pLox hPGK-Puro pA pLox

Plasmid

#171050

Purpose

Donor template for CRISPR/Cas9 targeting of TERT RE3

Depositor

Agnel Sfeir

Article

Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

Insert

Puromycin

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
xlox dNGFR-TERT

Plasmid

#69805

Purpose

MLV retroviral vector expressing the hTERT reverse transcriptase immortalizing gene and a tNGFR marker for tracking

Depositor

David Ott

Article

Andersen et al AIDS Res Hum Retroviruses. 2007 Mar;23(3

Insert

hTERT reverse transcriptase (TERT Human)

Use

Retroviral

Tags

none

Expression

Mutation

Promoter

MLV promoter

Available since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pInt-ERCreER

Plasmid

#190040

Purpose

This is an integration donor plasmid to insert a DNA fragment for doxycycline inducible ERT2-Cre-ERT2 at the AAVS1 locus. It works with an AAVS1 targeting CRISPR/Cas9 construct (Addgene #72833).

Depositor

Kayoko Tanaka

Article

Hindul et al Biol Open. 2022 Feb 15;11(2). pii: 27408

Insert

ERT2-Cre-ERT2 flanked by AAVS1

Use

CRISPR and TALEN

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI neo-hEST2

Plasmid

#1781

Depositor

Bob Weinberg

Article

Meyerson et al Cell 1997 Aug 22;90(4):785-95.

Insert

hTERT (TERT Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 20, 2005

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI neo-hEST2-HA

Plasmid

#1782

Depositor

Bob Weinberg

Article

Meyerson et al Cell 1997 Aug 22;90(4):785-95.

Insert

hTERT (TERT Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Clone is catalytically active but is dead in vivo.

Promoter

Available since

July 10, 2005

Availability

Academic Institutions and Nonprofits only