Skip to main content
Addgene
Showing: 21 - 40 of 322 results

  4. pCLxR6

    Plasmid
    #172557
    Purpose
    Inducible gene expression, pCOLA origin, LuxR activator, PLuxB promoter, mRFP, Streptomycin Resistance Marker.
    Depositor
    Christopher Reisch
    Insert
    LuxR-mRFP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PLuxB
    Available since
    Nov. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    172557-plasmid-map-sequence-id-346741

    Enlarge

  9. pCLAP

    Plasmid
    #200786
    Purpose
    Cloning template for N-terminal CLIPf tagging and C-terminal SNAPf tagging of constructs for mammalian expression. Also used for the mammalian expression of CLIPf-SNAPf-HisTag-FLAG fusion protein.
    Depositor
    Alison Twelvetrees
    Article
    Smith et al bioRxiv 2024.12.20.629623
    Type
    Empty backbone
    Use
    Tags
    CLIPf and SNAPf-6xHis-FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    200786-plasmid-map-sequence-id-435692

    Enlarge

  11. pCL1177

    Plasmid
    #217961
    Purpose
    Dual cistronic expression vector for the RAPID-release approach. H3.2-mCherry is released from mitochondria to the nucleus upon addition of rapamycin.
    Depositor
    Andrew Bowman
    Article
    Pardal et al Elife. 2022 Sep 6;11:e81755. doi: 10.755
    Insert
    Histone H3 (HIST2H3PS2 Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    217961-plasmid-map-sequence-id-430215

    Enlarge

  12. pCL1318

    Plasmid
    #217960
    Purpose
    Expression of human H4 carrying three point mutations to disrupt binding with H3. Expression in mammalian cells driven by the CMV promoter.
    Depositor
    Andrew Bowman
    Article
    Pardal et al Elife. 2022 Sep 6;11:e81755. doi: 10.755
    Insert
    Histone H4 (H4C1 Human)
    Use
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    L63A, F66A, I71A
    Promoter
    CMV
    Available since
    April 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    217960-plasmid-map-sequence-id-430214

    Enlarge

  13. pCL1317

    Plasmid
    #217959
    Purpose
    Expression of human H4 carrying a triple glycine insertion to disrupt folding with H3. Expression in mammalian cells driven by the CMV promoter.
    Depositor
    Andrew Bowman
    Article
    Pardal et al Elife. 2022 Sep 6;11:e81755. doi: 10.755
    Insert
    Histone H4 (H4C1 Human)
    Use
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    GGG insertion between V65 and F66
    Promoter
    CMV
    Available since
    April 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    217959-plasmid-map-sequence-id-430213

    Enlarge

  14. pCL1315

    Plasmid
    #217957
    Purpose
    Expression of human H3.1 carrying a triple point mutation to disrupt folding with histone H4. Expression in mammalian cells driven by the CMV promoter.
    Depositor
    Andrew Bowman
    Article
    Pardal et al Elife. 2022 Sep 6;11:e81755. doi: 10.755
    Insert
    Histone H3.1 (H3C1 Human)
    Use
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    F84A, L92A, Y99A
    Promoter
    CMV
    Available since
    April 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    217957-plasmid-map-sequence-id-430211

    Enlarge

  15. pCL1314

    Plasmid
    #217956
    Purpose
    Expression of human H3.1 carrying a triple glycine insertion to disrupt folding with H4. Expression in mammalian cells driven by the CMV promoter.
    Depositor
    Andrew Bowman
    Article
    Pardal et al Elife. 2022 Sep 6;11:e81755. doi: 10.755
    Insert
    Histone H3.1 (H3C1 Human)
    Use
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    GGG insertion between A95 and C96
    Promoter
    CMV
    Available since
    April 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    217956-plasmid-map-sequence-id-430210

    Enlarge

  17. pCLxR2

    Plasmid
    #172550
    Purpose
    Inducible gene expression, pCOLA origin, LuxR activator, PLuxB promoter, mRFP, Kanamycin Resistance Marker.
    Depositor
    Christopher Reisch
    Insert
    LuxR-mRFP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PLuxB
    Available since
    Dec. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    172550-plasmid-map-sequence-id-350265

    Enlarge

  18. pCLxR1

    Plasmid
    #172567
    Purpose
    Inducible gene expression, pCOLA origin, LuxR activator, PLuxB promoter, mRFP, Chloramphenicol Resistance Marker.
    Depositor
    Christopher Reisch
    Insert
    LuxR-mRFP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PLuxB
    Available since
    Nov. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    172567-plasmid-map-sequence-id-347593

    Enlarge

  19. pCLlR2

    Plasmid
    #172553
    Purpose
    Inducible gene expression, pCOLA origin, LacI repressor, PLlacO-1 promoter, mRFP, Kanamycin Resistance Marker.
    Depositor
    Christopher Reisch
    Insert
    LacI-mRFP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PLlacO-1
    Available since
    Oct. 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    172553-plasmid-map-sequence-id-346591

    Enlarge

  20. pCLxR4

    Plasmid
    #172543
    Purpose
    Inducible gene expression, pCOLA origin, LuxR activator, PLuxB promoter, mRFP, Carbenicillin Resistance Marker.
    Depositor
    Christopher Reisch
    Insert
    LuxR-mRFP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PLuxB
    Available since
    Sept. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    172543-plasmid-map-sequence-id-342845

    Enlarge

Showing: 21 - 40 of 322 results